Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,240,242 T→G 7.9% R16S (AGA→AGC)  PP_3717 ← LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,240,2420TG 7.9% 188.6 / 6.9 63R16S (AGA→AGC) PP_3717LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (32/26);  new base G (3/2);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

CTGGCCGATGGCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGTGATGAT  >  NC_002947/4240095‑4240390
                                                                                                                                                   |                                                                                                                                                    
cTGGCCGATGGCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTc                                                                                                                                                     >  7:250896/1‑149 (MQ=255)
  ggCCGATGGCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTa                                                                                                                                                   >  6:66950/1‑149 (MQ=255)
    ccGATGGCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGc                                                                                                                                                 <  1:163802/149‑1 (MQ=255)
          gCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGc                                                                                                                                             >  7:110250/1‑147 (MQ=255)
             gCGGCCACATACTCCAGCGCCTCGTGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGc                                                                                                                                        <  7:216811/149‑1 (MQ=255)
                         tCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGca                                                                                                                             >  5:185227/1‑148 (MQ=255)
                           cAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAg                                                                                                                          >  5:302859/1‑149 (MQ=255)
                            aGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCTGGCAGGCTAGACTGCCGGGCatgac                                                                                                                                   <  2:66782/139‑5 (MQ=255)
                            aGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCTGGCAGGCTAGACTGCCGGGCatgac                                                                                                                                   >  1:66782/1‑135 (MQ=255)
                             gcgcCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGcc                                                                                                                        >  4:205435/1‑149 (MQ=255)
                                      gggTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGcc                                                                                                               <  8:250896/149‑1 (MQ=255)
                                       ggTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCa                                                                                                              <  7:134565/149‑1 (MQ=255)
                                             aCCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGtt                                                                                                          >  3:17582/1‑147 (MQ=255)
                                               cATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATc                                                                                                      >  7:69953/1‑149 (MQ=255)
                                                      cAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTg                                                                                               <  3:213833/149‑1 (MQ=255)
                                                      cAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTg                                                                                               <  4:17582/149‑1 (MQ=255)
                                                          gcgcgcTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTa                                                                                            >  7:80657/1‑148 (MQ=255)
                                                           cgcgcTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACa                                                                                          <  8:110250/149‑1 (MQ=255)
                                                               cTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGc                                                                                       >  7:157519/1‑148 (MQ=255)
                                                                tGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGt                                                                                                                                                      <  3:229587/84‑1 (MQ=255)
                                                                tGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGt                                                                                                                                                      >  4:229587/1‑84 (MQ=255)
                                                                  cAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCaa                                                                                    <  5:66950/148‑1 (MQ=255)
                                                                   aaCGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAgc                                                                                  >  8:45005/1‑149 (MQ=255)
                                                                   aaCGCCACGCGCCACTGCTGCAGCGCTCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAgc                                                                                  <  3:166377/149‑1 (MQ=255)
                                                                        cACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGt                                                                             >  8:86873/1‑149 (MQ=255)
                                                                           gTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAg                                                                          >  3:110817/1‑149 (MQ=255)
                                                                              gtggcgttgcAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTc                                                                       <  5:265947/142‑1 (MQ=255)
                                                                              ccACTGCTGCTGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCTTCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTc                                                                       <  3:205435/149‑1 (MQ=255)
                                                                              ccACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGtt                                                                        >  5:248693/1‑148 (MQ=255)
                                                                                      gcAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCgg                                                               >  2:110480/1‑149 (MQ=255)
                                                                                       cAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCcgcg                                                                                                                               >  5:174593/1‑84 (MQ=255)
                                                                                       cAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCcgcg                                                                                                                               <  6:174593/84‑1 (MQ=255)
                                                                                       cAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGt                                                              >  4:253295/1‑149 (MQ=255)
                                                                                             gcCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAat                                                        >  5:194596/1‑149 (MQ=255)
                                                                                               ccATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAatga                                                      >  7:103979/1‑149 (MQ=255)
                                                                                               ccATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAat                                                        <  3:295116/147‑1 (MQ=255)
                                                                                               ccATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAat                                                        >  4:295116/1‑147 (MQ=255)
                                                                                                cATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAatgat                                                     <  3:253295/149‑1 (MQ=255)
                                                                                                 aTTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTTGGTCATTCCAGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAatgatg                                                    <  6:194596/149‑1 (MQ=255)
                                                                                                 aTTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAatgatg                                                    >  8:79957/1‑149 (MQ=255)
                                                                                                 aTTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAatgatg                                                    >  6:56125/1‑149 (MQ=255)
                                                                                                 aTTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAatgatg                                                    <  8:113307/149‑1 (MQ=255)
                                                                                                      gAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAg                                                                          >  8:141446/1‑122 (MQ=255)
                                                                                                      gAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAg                                                                          <  7:141446/122‑1 (MQ=255)
                                                                                                       aGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTgg                                              >  6:223510/1‑149 (MQ=255)
                                                                                                        gggTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGgc                                             <  7:45005/149‑1 (MQ=255)
                                                                                                          gTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGgcg                                            >  2:140855/1‑148 (MQ=255)
                                                                                                             aGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAg                                         <  7:79957/148‑1 (MQ=255)
                                                                                                              gCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCaa                                                                                    >  4:95025/1‑104 (MQ=255)
                                                                                                              gCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCaa                                                                                    <  3:95025/104‑1 (MQ=255)
                                                                                                              gCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCCAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAggg                                       >  4:43329/1‑149 (MQ=255)
                                                                                                              gCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTTAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAgg                                        <  1:108059/148‑1 (MQ=255)
                                                                                                                ggggTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGtc                                     >  7:267463/1‑149 (MQ=255)
                                                                                                                      tCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTcgacg                               >  7:266067/1‑149 (MQ=255)
                                                                                                                       cGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTcgacga                              >  5:151131/1‑149 (MQ=255)
                                                                                                                               gCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTc                       >  3:15808/1‑148 (MQ=255)
                                                                                                                                     gTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTgc                <  6:302859/149‑1 (MQ=255)
                                                                                                                                         tGCCTGGCAGGCTAGACTGCCGGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCAt            >  2:208858/1‑149 (MQ=255)
                                                                                                                                          gCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGtct                                    <  5:82661/124‑1 (MQ=255)
                                                                                                                                          gCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGtct                                    >  6:82661/1‑124 (MQ=255)
                                                                                                                                            ctggCAGGCTAGACTGCCGGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATggg         >  3:85940/3‑149 (MQ=255)
                                                                                                                                             cGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGt        <  8:266067/149‑1 (MQ=255)
                                                                                                                                               gCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGtga      <  8:80657/149‑1 (MQ=255)
                                                                                                                                               gCAGGCTAGACTGCCGGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGtg       <  4:85940/148‑1 (MQ=255)
                                                                                                                                                 aGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGtgatg    <  6:248693/149‑1 (MQ=255)
                                                                                                                                                   tCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGTgatgat  >  7:67118/1‑149 (MQ=255)
                                                                                                                                                   tCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGTgatgat  <  8:267463/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTGGCCGATGGCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGTGATGAT  >  NC_002947/4240095‑4240390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑