Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,248,056	G→T	5.4%	A657A (GCC→GCA)	PP_3724 ←	acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,248,056	0	G	T	5.4%	243.2 / 3.4	74	A657A (GCC→GCA)	PP_3724	acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (36/34);  new base T (2/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.74e-01

GTCATTCATGTCGTTCTCCTTGCGCCTGGCCAAGGCGGATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGCTGACACTGTCCTTGAGCAGTTCGAC  >  NC_002947/4247908‑4248202                                                                                                                                                     |                                                                                                                                                     gTCATTCATGTCGTTCTCCTTGCGCCTGGCCAAGGCGGATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATg                                                                                                                                                      <  4:40979/149‑1 (MQ=255) gTCATTCATGTCGTTCTCCTTGCGCCTGGCCAAGGCGGATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATg                                                                                                                                                      >  2:28035/1‑149 (MQ=255)     ttcatGTCGTTCTCCTTGCGCCTGGCCAAGGCGGATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGcc                                                                                                                                                   >  3:216262/1‑148 (MQ=255)      tcatGTCGTTCTCCTTGCGCCTGGCCAAGGCGGATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGcc                                                                                                                                                   >  5:180048/1‑147 (MQ=255)          gTCGTTCTCCTTGCGCCTGGCCAAGGCGGATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGAc                                                                                                                                              >  3:213967/1‑148 (MQ=255)                   cTTGCGCCTGGCCAAGGCGGATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATc                                                                                                                                     <  2:287895/148‑1 (MQ=255)                        gcCTGGCCAAGGCGGATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGtt                                                                                                                               >  8:201019/1‑149 (MQ=255)                            ggCCAAGGCGGATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑ggcg                                                                                                                          >  7:63207/1‑149 (MQ=255)                             gCCAAGGCGGATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCACGGATGGCCGAGACCAAGGCATCGAGGTT‑ggcg                                                                                                                          <  6:175217/148‑1 (MQ=255)                                 aGGCGGATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAg                                                                                                                                  <  2:64224/137‑1 (MQ=255)                                    cGGATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACg                                                                                                                  <  4:213967/149‑1 (MQ=255)                                     ggATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑g                                                                                                                             <  5:46045/137‑1 (MQ=255)                                     ggATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑g                                                                                                                             >  6:46045/1‑137 (MQ=255)                                           gcgcGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATTGCCGAGACCAAGGCATCGAGGTT‑GGTGGCCGGACG‑GCCACg                                                                                                           >  4:291993/1‑148 (MQ=255)                                           gcgcGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATTGCCGAGACCAAGGCATCGAGGTT‑GGTGGCCGGACG‑GCCACg                                                                                                           >  4:287530/1‑148 (MQ=255)                                           gcgcGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACg                                                                                                           >  8:235069/1‑148 (MQ=255)                                             gcgTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGaa                                                                                                        <  4:127980/149‑1 (MQ=255)                                                    cTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATTGCCGAGACCAAGGCATCGAGGTT‑GGTGGCCGGACG‑GCCACGGAAGCCTTgc                                                                                                 <  3:287530/149‑1 (MQ=255)                                                    cTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATTGCCGAGACCAAGGCATCGAGGTT‑GGTGGCCGGACG‑GCCACGGAAGCCTTgc                                                                                                 <  3:291993/149‑1 (MQ=255)                                                    cTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTgc                                                                                                 <  7:201019/149‑1 (MQ=255)                                                     tGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATAAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTg                                                                                                  >  8:153997/1‑147 (MQ=255)                                                       cGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTgcag                                                                                               >  2:10544/1‑148 (MQ=255)                                                       cGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTgca                                                                                                <  7:235069/147‑1 (MQ=255)                                                       cGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTgca                                                                                                <  7:153997/147‑1 (MQ=255)                                                        ggtggtACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTgcag                                                                                               >  2:22789/1‑147 (MQ=255)                                                                  ttGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACGCGCCACGGAAGCCTTGCAGCAGGCTGGCAc                                                                                    <  1:22789/149‑1 (MQ=255)                                                                     gCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GTCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTgc                                                                                 <  8:146755/148‑1 (MQ=255)                                                                         cGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAgg                                                                            >  4:134098/1‑149 (MQ=255)                                                                           aCCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTgcg                                                                                <  5:116470/143‑1 (MQ=255)                                                                           aCCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTgcg                                                                                >  6:116470/1‑143 (MQ=255)                                                                             cATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGtt                                                                          >  3:143601/1‑147 (MQ=255)                                                                              aTCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTcag                                                                       <  3:148180/149‑1 (MQ=255)                                                                              aTCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTcag                                                                       <  3:134098/149‑1 (MQ=255)                                                                                    gggTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTc                                                                         >  8:107127/1‑141 (MQ=255)                                                                                    gggTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTc                                                                         <  7:107127/141‑1 (MQ=255)                                                                                     ggTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTgcg                                                                <  7:97363/149‑1 (MQ=255)                                                                                      gTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTgcgg                                                               >  5:178733/1‑149 (MQ=255)                                                                                      gTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTgcgg                                                               >  5:124731/1‑149 (MQ=255)                                                                                      gTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTgcgg                                                               >  1:14976/1‑149 (MQ=255)                                                                                       ttCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTgcggc                                                              >  8:102837/1‑149 (MQ=255)                                                                                          acaTCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGcgcg                                                           <  4:143601/149‑1 (MQ=255)                                                                                               cAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATgg                                                      <  8:63207/149‑1 (MQ=255)                                                                                               cAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCAGCGCGGATgg                                                      <  1:28035/149‑1 (MQ=255)                                                                                                aGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGaa                                                                                                        >  4:139523/1‑98 (MQ=255)                                                                                                      aTCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTc                                               <  1:10544/149‑1 (MQ=255)                                                                                                       tCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCg                                              >  4:252453/1‑149 (MQ=255)                                                                                                       tCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCg                                              >  3:47996/1‑149 (MQ=255)                                                                                                         aaCTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCgg                                             >  5:78553/1‑148 (MQ=255)                                                                                                          aCTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCgg                                             >  8:104659/1‑147 (MQ=255)                                                                                                          aCTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTa                                           <  4:47996/149‑1 (MQ=255)                                                                                                           cTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAg                                          >  2:290040/1‑149 (MQ=255)                                                                                                              cccGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTTGGGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTg                                        >  1:245836/1‑149 (MQ=255)                                                                                                              cccGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTg                                        >  1:220696/1‑148 (MQ=255)                                                                                                              cccGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTg                                        <  2:220696/148‑1 (MQ=255)                                                                                                                cGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCgg                                             >  4:215549/1‑141 (MQ=255)                                                                                                                cGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCgg                                             <  3:215549/141‑1 (MQ=255)                                                                                                                 ggCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTggg                                      >  4:287229/1‑147 (MQ=255)                                                                                                                 ggCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGgca                                    <  3:287229/149‑1 (MQ=255)                                                                                                                  gCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGgcag                                   >  3:30092/1‑149 (MQ=255)                                                                                                                         cGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAg                             <  5:248340/148‑1 (MQ=255)                                                                                                                         cGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAg                             >  6:248340/1‑148 (MQ=255)                                                                                                                          gCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGc                           <  7:102837/149‑1 (MQ=255)                                                                                                                            aGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGCTg                         <  6:180048/149‑1 (MQ=255)                                                                                                                             ggCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGCTGa                        <  6:78553/149‑1 (MQ=255)                                                                                                                                  aaTCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCCGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGCTGACACTg                   >  8:161828/1‑149 (MQ=255)                                                                                                                                    tCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGCCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGCTGACACTGTc                 >  6:305272/1‑149 (MQ=255)                                                                                                                                         gACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAg                             <  3:280222/132‑1 (MQ=255)                                                                                                                                         gACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAg                             >  4:280222/1‑132 (MQ=255)                                                                                                                                         gACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGCTGACACTGTCCTTGa            <  6:178733/149‑1 (MQ=255)                                                                                                                                         gACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGCTGACACTGTCCTTGa            <  4:216262/149‑1 (MQ=255)                                                                                                                                         gACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGCTGACACTGTCCTTGa            <  1:290040/149‑1 (MQ=255)                                                                                                                                            tGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGCTGACACTGTCCTTGAg           >  5:263994/1‑147 (MQ=255)                                                                                                                                               gcgGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGCTGACACTGTCCTTGAGCAGtt      <  3:91536/149‑1 (MQ=255)                                                                                                                                               gcgGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGCTGACACTGTCCTTGAGCAGt       >  4:91536/1‑148 (MQ=255)                                                                                                                                                  gATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGTCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGCTGACACTGTCCTTGAGCAGTTCGa   >  5:230875/1‑149 (MQ=255)                                                                                                                                                   aTGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGCTGACACTGTCCTTGAGCAGTTCGAc  <  6:124731/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GTCATTCATGTCGTTCTCCTTGCGCCTGGCCAAGGCGGATCAGCGCGTCGACTGCGGTGGTACCGTTGGCACCGACCATCAATGGGTTCACATCCAGCTCCATCAACTGCCCGGCGTTTTCGCAGGCGTAATCGGCGACTGCGCGGATGGCCGAGACCAAGGCATCGAGGTT‑GGCGGCCGGACG‑GCCACGGAAGCCTTGCAGCAGGCTGGCACTGCGCAGGTTCAGCAGTGCGGCGCGGATGGCGCTGTCGGTAGTGGGCAGCAACAGGCTGACACTGTCCTTGAGCAGTTCGAC  >  NC_002947/4247908‑4248202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑