Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,258,891 T→C 7.5% R146R (CGT→CGC) ‡ PP_3732 → enoyl‑CoA hydratase/isomerase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,258,8910TC 7.5% 150.8 / 2.3 53R146R (CGT→CGC) ‡PP_3732enoyl‑CoA hydratase/isomerase family protein
Reads supporting (aligned to +/- strand):  ref base T (26/23);  new base C (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

CAGCGGTCAGTGGTGCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCACGCTGGCGCGGCAGC  >  NC_002947/4258743‑4259036
                                                                                                                                                    |                                                                                                                                                 
cAGCGGTCAGTGGTGCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCgt                                                                                                                                                   >  4:141085/1‑149 (MQ=255)
      tCAGTGGTGCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCtt                                                                                                                                             >  6:312707/1‑149 (MQ=255)
       cAGTGGTGCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCgtgagatcg                                                                                                                                            >  7:20724/1‑143 (MQ=255)
       cAGTGGTGCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCgt                                                                                                                                                   <  8:20724/142‑1 (MQ=255)
        aGTGGTGCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTg                                                                                                                                            <  5:293120/148‑1 (MQ=255)
            gtgCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAg                                                                                                                                       >  1:194945/1‑149 (MQ=255)
             tgCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCCCGGGCCTGGCCaag                                                                                                                                      >  2:57744/1‑147 (MQ=255)
                 gcgATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCg                                                                                                                                  <  7:142594/149‑1 (MQ=255)
                  cgATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCgg                                                                                                                                 <  3:21421/149‑1 (MQ=255)
                             ggggCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCCCGGGCCTGGCCAAGCACACGCGGCagcag                                                                                                                       <  1:57744/148‑5 (MQ=38)
                                gCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTtgct                                                                                                                   <  6:166819/149‑1 (MQ=255)
                                gCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTtgct                                                                                                                   <  8:262932/149‑1 (MQ=255)
                                gCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTtgct                                                                                                                   <  2:194945/149‑1 (MQ=255)
                                    cccTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTgctgct                                                                                                                >  1:28180/1‑148 (MQ=255)
                                          tgctgcACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAAt                                                                                                         >  1:148481/1‑149 (MQ=255)
                                            ctgcACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATg                                                                                                        >  8:136547/1‑148 (MQ=255)
                                             tgcACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCa                                                                                                      >  2:152189/1‑149 (MQ=255)
                                               cACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCAtt                                                                                                    >  2:73096/1‑149 (MQ=255)
                                               cACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCAt                                                                                                     <  3:141085/148‑1 (MQ=255)
                                                    cGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTgg                                                                                               <  2:28180/149‑1 (MQ=255)
                                                           gTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGc                                                                                                                        >  7:278286/1‑117 (MQ=255)
                                                           gTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGc                                                                                                                        <  8:278286/117‑1 (MQ=255)
                                                                ggTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGaa                                                                                       >  1:222414/1‑145 (MQ=255)
                                                                 gTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGAt                                                                                  >  3:96029/1‑149 (MQ=255)
                                                                 gTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGAt                                                                                  >  3:3114/1‑149 (MQ=255)
                                                                  tCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATg                                                                                 <  1:73096/149‑1 (MQ=255)
                                                                  tCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATg                                                                                 <  7:112565/149‑1 (MQ=255)
                                                                      ccGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTgg                                                                             >  3:40987/1‑149 (MQ=255)
                                                                       cGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGc                                                                            >  5:28017/1‑149 (MQ=255)
                                                                            gACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTggg                                                                       <  5:312707/149‑1 (MQ=255)
                                                                                  gCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTgg                                                                 <  6:28017/149‑1 (MQ=255)
                                                                                       gcATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAAt                                                            <  4:96029/149‑1 (MQ=255)
                                                                                          tGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTCGATCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAg                                                         >  4:102482/1‑149 (MQ=255)
                                                                                                 cgcgCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCAt                                                   <  2:148481/148‑1 (MQ=255)
                                                                                                     ccgcTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGaaaa                                              >  5:34813/1‑149 (MQ=255)
                                                                                                         tGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGc                                          <  6:24654/149‑1 (MQ=255)
                                                                                                              gtgtgCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGaa                                       >  8:278674/1‑147 (MQ=255)
                                                                                                              gtgtgCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACa                                     <  4:3114/149‑1 (MQ=255)
                                                                                                                gtgCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGt                                   >  7:101565/1‑149 (MQ=255)
                                                                                                                   cccGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGcc                                >  5:131541/1‑149 (MQ=255)
                                                                                                                   cccGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGcc                                >  4:222991/1‑149 (MQ=255)
                                                                                                                     cGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTg                              <  6:34813/149‑1 (MQ=255)
                                                                                                                          tttGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGc                         <  6:131541/149‑1 (MQ=255)
                                                                                                                               ttCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTg                                                                              >  5:99664/1‑91 (MQ=255)
                                                                                                                               ttCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTg                                                                              >  5:95501/1‑91 (MQ=255)
                                                                                                                               ttCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTg                                                                              <  6:95501/91‑1 (MQ=255)
                                                                                                                               ttCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTg                                                                              <  6:99664/91‑1 (MQ=255)
                                                                                                                                tCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCg                   >  6:234765/1‑149 (MQ=255)
                                                                                                                                  cAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCa                 >  6:222244/1‑149 (MQ=255)
                                                                                                                                  cAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCa                 >  4:202850/1‑149 (MQ=255)
                                                                                                                                         ctgctgcCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCACGCTgg           <  5:222244/148‑1 (MQ=255)
                                                                                                                                          gcgtgcCGCCCGGGCCTGGCCAAGCACACGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCaa                                                             >  2:293298/4‑97 (MQ=21)
                                                                                                                                          gcgtgcCGCCCGGGCCTGGCCAAGCACACGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCaa                                                             <  1:293298/94‑1 (MQ=21)
                                                                                                                                            ctgcCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCACGCTGgcgc        <  8:101565/148‑1 (MQ=255)
                                                                                                                                             tgcCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCACGCTGGCGCgg      >  5:181143/1‑149 (MQ=255)
                                                                                                                                                 gcgTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATGGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCACGCTGGCGCGGCAGc  >  1:75444/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CAGCGGTCAGTGGTGCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCACGCTGGCGCGGCAGC  >  NC_002947/4258743‑4259036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑