Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,258,893 T→G 7.5% V147G (GTG→GGG)  PP_3732 → enoyl‑CoA hydratase/isomerase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,258,8930TG 7.5% 162.8 / 3.5 53V147G (GTG→GGG) PP_3732enoyl‑CoA hydratase/isomerase family protein
Reads supporting (aligned to +/- strand):  ref base T (26/23);  new base G (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TCAGTGGTGCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCACGCTGGCGCGGCAGCTGCAA  >  NC_002947/4258749‑4259041
                                                                                                                                                |                                                                                                                                                    
tCAGTGGTGCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCtt                                                                                                                                                  >  6:312707/1‑149 (MQ=255)
  aGTGGTGCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTg                                                                                                                                                 <  5:293120/148‑1 (MQ=255)
      gtgCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAg                                                                                                                                            >  1:194945/1‑149 (MQ=255)
       tgCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCCCGGGCCTGGCCaag                                                                                                                                           >  2:57744/1‑147 (MQ=255)
           gcgATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCg                                                                                                                                       <  7:142594/149‑1 (MQ=255)
            cgATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCgg                                                                                                                                      <  3:21421/149‑1 (MQ=255)
                       ggggCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCCCGGGCCTGGCCAAGCACACGCGGCagcag                                                                                                                            <  1:57744/148‑5 (MQ=38)
                          gCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTtgct                                                                                                                        <  6:166819/149‑1 (MQ=255)
                          gCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTtgct                                                                                                                        <  2:194945/149‑1 (MQ=255)
                          gCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTtgct                                                                                                                        <  8:262932/149‑1 (MQ=255)
                              cccTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTgctgct                                                                                                                     >  1:28180/1‑148 (MQ=255)
                                    tgctgcACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAAt                                                                                                              >  1:148481/1‑149 (MQ=255)
                                      ctgcACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATg                                                                                                             >  8:136547/1‑148 (MQ=255)
                                       tgcACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCa                                                                                                           >  2:152189/1‑149 (MQ=255)
                                         cACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCAtt                                                                                                         >  2:73096/1‑149 (MQ=255)
                                         cACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCAt                                                                                                          <  3:141085/148‑1 (MQ=255)
                                              cGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTgg                                                                                                    <  2:28180/149‑1 (MQ=255)
                                                     gTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGc                                                                                                                             <  8:278286/117‑1 (MQ=255)
                                                     gTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGc                                                                                                                             >  7:278286/1‑117 (MQ=255)
                                                          ggTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGaa                                                                                            >  1:222414/1‑145 (MQ=255)
                                                           gTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGAt                                                                                       >  3:96029/1‑149 (MQ=255)
                                                           gTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGAt                                                                                       >  3:3114/1‑149 (MQ=255)
                                                            tCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATg                                                                                      <  7:112565/149‑1 (MQ=255)
                                                            tCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATg                                                                                      <  1:73096/149‑1 (MQ=255)
                                                                ccGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTgg                                                                                  >  3:40987/1‑149 (MQ=255)
                                                                 cGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGc                                                                                 >  5:28017/1‑149 (MQ=255)
                                                                      gACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTggg                                                                            <  5:312707/149‑1 (MQ=255)
                                                                            gCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTgg                                                                      <  6:28017/149‑1 (MQ=255)
                                                                                 gcATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAAt                                                                 <  4:96029/149‑1 (MQ=255)
                                                                                    tGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTCGATCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAg                                                              >  4:102482/1‑149 (MQ=255)
                                                                                           cgcgCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCAt                                                        <  2:148481/148‑1 (MQ=255)
                                                                                               ccgcTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGaaaa                                                   >  5:34813/1‑149 (MQ=255)
                                                                                                   tGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGc                                               <  6:24654/149‑1 (MQ=255)
                                                                                                        gtgtgCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGaa                                            >  8:278674/1‑147 (MQ=255)
                                                                                                        gtgtgCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACa                                          <  4:3114/149‑1 (MQ=255)
                                                                                                          gtgCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGt                                        >  7:101565/1‑149 (MQ=255)
                                                                                                             cccGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGcc                                     >  5:131541/1‑149 (MQ=255)
                                                                                                             cccGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGcc                                     >  4:222991/1‑149 (MQ=255)
                                                                                                               cGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTg                                   <  6:34813/149‑1 (MQ=255)
                                                                                                                    tttGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGc                              <  6:131541/149‑1 (MQ=255)
                                                                                                                         ttCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTg                                                                                   >  5:99664/1‑91 (MQ=255)
                                                                                                                         ttCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTg                                                                                   >  5:95501/1‑91 (MQ=255)
                                                                                                                         ttCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTg                                                                                   <  6:95501/91‑1 (MQ=255)
                                                                                                                         ttCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTg                                                                                   <  6:99664/91‑1 (MQ=255)
                                                                                                                          tCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCg                        >  6:234765/1‑149 (MQ=255)
                                                                                                                            cAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCa                      >  6:222244/1‑149 (MQ=255)
                                                                                                                            cAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCa                      >  4:202850/1‑149 (MQ=255)
                                                                                                                                   ctgctgcCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCACGCTgg                <  5:222244/148‑1 (MQ=255)
                                                                                                                                    gcgtgcCGCCCGGGCCTGGCCAAGCACACGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCaa                                                                  <  1:293298/94‑1 (MQ=21)
                                                                                                                                    gcgtgcCGCCCGGGCCTGGCCAAGCACACGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCaa                                                                  >  2:293298/4‑97 (MQ=21)
                                                                                                                                      ctgcCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCACGCTGgcgc             <  8:101565/148‑1 (MQ=255)
                                                                                                                                       tgcCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCACGCTGGCGCgg           >  5:181143/1‑149 (MQ=255)
                                                                                                                                           gcgTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATGGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCACGCTGGCGCGGCAGc       >  1:75444/1‑149 (MQ=255)
                                                                                                                                               gtgCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCACGCTGGCGCGGCAGCTGc    >  6:115750/1‑148 (MQ=255)
                                                                                                                                                tgCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCACGCTGGCGCGGCAGCTGCaa  <  3:102482/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TCAGTGGTGCGGCGATCGGCATCGGGGCCACCCTGTTGCTGCACTGCGACCAGGTGCTGGTCAGCCGCTCGACCAAGCTGCGCATGCCTTTCGCGCCGCTGGGTGTGTGCCCGGAGTTTGGTTCCAGCCTGCTGCTGCCGCGTGTGCTTGGCCAGGCCCGGGCGGCACGCTTGCTGCTGACCAATGCATTGCTGGATGGCGAACAGATGCTGGCCTGGGGGCTGGCCAATGAGCTGCATGAAAACGGCGAACAGTGCCTGGAAGCTGCCCGCACGCTGGCGCGGCAGCTGCAA  >  NC_002947/4258749‑4259041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑