Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,264,883 C→G 10.0% F32L (TTC→TTG) ‡ vanB → vanillate O‑demethylase oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,264,8830CG10.0% 107.8 / 4.8 40F32L (TTC→TTG) ‡vanBvanillate O‑demethylase oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (16/20);  new base G (2/2);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01

GCAGCCGCAACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGC  >  NC_002947/4264736‑4265025
                                                                                                                                                   |                                                                                                                                              
gcagccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCa                                                                                                                                               <  5:170788/149‑1 (MQ=255)
     cgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGcc                                                                                                                                          >  2:308242/1‑149 (MQ=255)
       caACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCgg                                                                                                                                        >  8:76691/1‑149 (MQ=255)
        aaCTGATCGCCCCCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGCTGAACGCCGgc                                                                                                                                       <  2:199437/149‑1 (MQ=255)
             aTCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGC‑GCCGGCGCTGAACGCCGgcagcagg                                                                                                                                 <  5:128067/149‑7 (MQ=255)
              tCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCa                                                                                                                                  >  5:154589/1‑148 (MQ=255)
                    ccaGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGAc                                                                                                                           >  1:117780/1‑149 (MQ=255)
                          ccAACCCTGCATGAGTAACAGCAGACATGATCGATGCCGTATTGGTATCACGTAACGATGAAGCGAAGGGTATCTGCAGCTTCTAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCTCCGGCGCCCATATCTACTTGCAc                                                                                                                     <  1:269808/149‑1 (MQ=255)
                                           aCAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGGTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGt                                                                                                    <  7:252882/149‑1 (MQ=255)
                                                      gATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTAtt                                                                                         >  1:60865/1‑149 (MQ=255)
                                                        tCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCg                                                                                       <  1:55558/149‑1 (MQ=255)
                                                          gATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCt                                                                                     <  2:117780/149‑1 (MQ=255)
                                                           ttGCCGTAGTGGTATCCCGTAACGATGACGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCtg                                                                                    <  4:280396/148‑1 (MQ=255)
                                                               cGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCa                                                                                <  5:107183/149‑1 (MQ=255)
                                                                      gTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCAccc                                                                         <  1:308242/149‑1 (MQ=255)
                                                                         tCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCCGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCg                                                                       >  4:33292/1‑148 (MQ=255)
                                                                              tAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAAc                                                                 <  1:191760/149‑1 (MQ=255)
                                                                                         cgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTAt                                                      >  6:249885/1‑149 (MQ=255)
                                                                                                    tGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAac                                                                              >  1:74068/1‑114 (MQ=255)
                                                                                                    tGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAac                                                                              <  2:74068/114‑1 (MQ=255)
                                                                                                    tGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGAtt                                                >  4:160769/1‑144 (MQ=255)
                                                                                                    tGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGAtt                                                <  3:160769/144‑1 (MQ=255)
                                                                                                         cTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTc                                       >  7:13390/1‑148 (MQ=255)
                                                                                                          ttCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCaa                                     <  7:76691/149‑1 (MQ=255)
                                                                                                                cTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGAccc                               >  8:280877/1‑149 (MQ=255)
                                                                                                                  ggCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCgg                             >  5:210040/1‑149 (MQ=255)
                                                                                                                   gCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGAccctg                             <  6:154589/148‑3 (MQ=255)
                                                                                                                     cgcgGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCCAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCtt                          >  4:65878/1‑149 (MQ=255)
                                                                                                                            gATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg                   >  8:234311/1‑149 (MQ=255)
                                                                                                                              tGGCAGCCTGCTGCCGTCGTTCAGCGACGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCg                 <  4:61180/149‑1 (MQ=255)
                                                                                                                              tGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCg                 <  8:13390/149‑1 (MQ=255)
                                                                                                                                 cAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGtt              <  5:249885/149‑1 (MQ=255)
                                                                                                                                    ccTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTtctc           <  2:60865/149‑1 (MQ=255)
                                                                                                                                      gggcgcCGGCGCTGAACGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCg          >  4:274110/5‑148 (MQ=255)
                                                                                                                                       gctgcCGGCGTTCAGCGCGGGCGCCCATATCGACGGGCACCTGCCCGACGTGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCTAAGACCGCCATCGCTTTCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTa        <  8:272934/149‑1 (MQ=255)
                                                                                                                                        gcgcCGGCGCTGAACGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAg       >  7:251488/3‑149 (MQ=255)
                                                                                                                                          gcCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGcc     >  2:96764/1‑149 (MQ=255)
                                                                                                                                             ggCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGc  <  3:33292/149‑1 (MQ=255)
                                                                                                                                                 ttCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTcgc                       >  3:154499/1‑124 (MQ=255)
                                                                                                                                                 ttCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTcgc                       <  4:154499/124‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GCAGCCGCAACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGC  >  NC_002947/4264736‑4265025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑