Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,264,885 G→A 10.0% S33N (AGC→AAC)  vanB → vanillate O‑demethylase oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,264,8850GA10.0% 103.5 / 2.7 40S33N (AGC→AAC) vanBvanillate O‑demethylase oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (17/19);  new base A (2/2);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.65e-01

CGCAACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAG  >  NC_002947/4264741‑4265033
                                                                                                                                                |                                                                                                                                                    
cgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGcc                                                                                                                                                  >  2:308242/1‑149 (MQ=255)
  caACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCgg                                                                                                                                                >  8:76691/1‑149 (MQ=255)
   aaCTGATCGCCCCCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGCTGAACGCCGgc                                                                                                                                               <  2:199437/149‑1 (MQ=255)
        aTCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGC‑GCCGGCGCTGAACGCCGgcagcagg                                                                                                                                         <  5:128067/149‑7 (MQ=255)
         tCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCa                                                                                                                                          >  5:154589/1‑148 (MQ=255)
               ccaGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGAc                                                                                                                                   >  1:117780/1‑149 (MQ=255)
                     ccAACCCTGCATGAGTAACAGCAGACATGATCGATGCCGTATTGGTATCACGTAACGATGAAGCGAAGGGTATCTGCAGCTTCTAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCTCCGGCGCCCATATCTACTTGCAc                                                                                                                             <  1:269808/149‑1 (MQ=255)
                                      aCAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGGTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGt                                                                                                            <  7:252882/149‑1 (MQ=255)
                                                 gATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTAtt                                                                                                 >  1:60865/1‑149 (MQ=255)
                                                   tCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCg                                                                                               <  1:55558/149‑1 (MQ=255)
                                                     gATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCt                                                                                             <  2:117780/149‑1 (MQ=255)
                                                      ttGCCGTAGTGGTATCCCGTAACGATGACGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCtg                                                                                            <  4:280396/148‑1 (MQ=255)
                                                          cGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCa                                                                                        <  5:107183/149‑1 (MQ=255)
                                                                 gTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCAccc                                                                                 <  1:308242/149‑1 (MQ=255)
                                                                    tCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCCGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCg                                                                               >  4:33292/1‑148 (MQ=255)
                                                                         tAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAAc                                                                         <  1:191760/149‑1 (MQ=255)
                                                                                    cgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTAt                                                              >  6:249885/1‑149 (MQ=255)
                                                                                               tGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAac                                                                                      <  2:74068/114‑1 (MQ=255)
                                                                                               tGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAac                                                                                      >  1:74068/1‑114 (MQ=255)
                                                                                               tGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGAtt                                                        >  4:160769/1‑144 (MQ=255)
                                                                                               tGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGAtt                                                        <  3:160769/144‑1 (MQ=255)
                                                                                                    cTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTc                                               >  7:13390/1‑148 (MQ=255)
                                                                                                     ttCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCaa                                             <  7:76691/149‑1 (MQ=255)
                                                                                                           cTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGAccc                                       >  8:280877/1‑149 (MQ=255)
                                                                                                             ggCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCgg                                     >  5:210040/1‑149 (MQ=255)
                                                                                                              gCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGAccctg                                     <  6:154589/148‑3 (MQ=255)
                                                                                                                cgcgGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCCAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCtt                                  >  4:65878/1‑149 (MQ=255)
                                                                                                                       gATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg                           >  8:234311/1‑149 (MQ=255)
                                                                                                                         tGGCAGCCTGCTGCCGTCGTTCAGCGACGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCg                         <  4:61180/149‑1 (MQ=255)
                                                                                                                         tGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCg                         <  8:13390/149‑1 (MQ=255)
                                                                                                                            cAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGtt                      <  5:249885/149‑1 (MQ=255)
                                                                                                                               ccTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTtctc                   <  2:60865/149‑1 (MQ=255)
                                                                                                                                 gggcgcCGGCGCTGAACGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCg                  >  4:274110/5‑148 (MQ=255)
                                                                                                                                  gctgcCGGCGTTCAGCGCGGGCGCCCATATCGACGGGCACCTGCCCGACGTGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCTAAGACCGCCATCGCTTTCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTa                <  8:272934/149‑1 (MQ=255)
                                                                                                                                   gcgcCGGCGCTGAACGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAg               >  7:251488/3‑149 (MQ=255)
                                                                                                                                     gcCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGcc             >  2:96764/1‑149 (MQ=255)
                                                                                                                                        ggCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGc          <  3:33292/149‑1 (MQ=255)
                                                                                                                                            ttCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTcgc                               >  3:154499/1‑124 (MQ=255)
                                                                                                                                            ttCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTcgc                               <  4:154499/124‑1 (MQ=255)
                                                                                                                                               aGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGGATTCGCTGTGCACCCACCCCGCAGCCCGCCATCGCTATCTGATTGGCGTCCTCCCCGCCCCGGCTGCGCGGGGCGGTTCGCGTAGCCTGCCCGAAcc   >  5:249005/1‑148 (MQ=255)
                                                                                                                                                gcgcCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAg  <  7:234311/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CGCAACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAG  >  NC_002947/4264741‑4265033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑