Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,301,579 G→C 9.6% intergenic (+69/‑1231) PP_3774 → / → PP_5627 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,301,5790GC 9.6% 111.4 / 4.6 42intergenic (+69/‑1231)PP_3774/PP_5627hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (18/19);  new base C (2/2);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

CACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCTCTGCCTGCCT  >  NC_002947/4301441‑4301727
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cacCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCCCCGCGCTGGGCttt                                                                                                                                            <  2:143303/149‑2 (MQ=255)
 acCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCtt                                                                                                                                             >  1:9071/1‑147 (MQ=255)
 acCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCtt                                                                                                                                             <  2:9071/147‑1 (MQ=255)
 acCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTc                                                                                                                                            >  5:222623/1‑148 (MQ=255)
     aCGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTAt                                                                                                                                         >  5:64274/1‑147 (MQ=255)
         aaCCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTg                                                                                                                                   >  5:54905/1‑149 (MQ=255)
          aCCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTgg                                                                                                                                  <  4:173872/149‑1 (MQ=255)
            ccccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCt                                                                                                                                    <  4:51187/145‑1 (MQ=255)
            ccccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCt                                                                                                                                    >  3:51187/1‑145 (MQ=255)
            ccccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGc                                                                                                                                <  6:64274/149‑1 (MQ=255)
            ccccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGc                                                                                                                                >  3:69720/1‑149 (MQ=255)
                gccgAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGaa                                                                                                                            <  1:250641/149‑1 (MQ=255)
                  cgAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGATGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACa                                                                                                                          <  7:171243/149‑1 (MQ=255)
                           cTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGATGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCg                                                                                                                 >  7:215155/1‑149 (MQ=255)
                              gATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGc                                                                                                                <  7:48068/147‑1 (MQ=255)
                                                  tctcGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTg                                                                                           >  1:4200/1‑148 (MQ=255)
                                                      gCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGaa                                                                                        <  6:54905/147‑1 (MQ=255)
                                                      gCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAg                                                                                       <  1:73266/148‑1 (MQ=255)
                                                          atgcttggCTGAACGTCGATGCCCAGCCCTGCTATCAATACCCCTCAGTGCGTCGCATGAATGCAAAAAGCCCAGCGCGTGGCTGGGCTTGTATCAGCTGGGCTGAACAGTAGTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACt                                                                                  <  7:93091/142‑1 (MQ=255)
                                                                  cTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTggg                                                                          >  8:99125/1‑149 (MQ=255)
                                                                       cGTCGATGCCCATCCCTGCTATCAACACCACTCAGGGCGTCTCATGAATGCAAAAATGCCACCGCGGTGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTAGACACTGTACTGGAAGTTACTCCATTGGGCGCtt                                                                     <  4:16959/149‑1 (MQ=255)
                                                                       cGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCtt                                                                     <  7:99125/149‑1 (MQ=255)
                                                                         tCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTtgt                                                                   <  2:4200/149‑1 (MQ=255)
                                                                                ccAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCtt                                                                     >  3:5057/1‑140 (MQ=255)
                                                                                ccAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCtt                                                                     <  4:5057/140‑1 (MQ=255)
                                                                                ccAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAAc                                                             >  7:93827/1‑148 (MQ=255)
                                                                                ccAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCAGAACAGTACTTCCGCCAGCCTATACACACTGTACTGGAAGTTACTACAGAGGGCGCTTGTGATAAc                                                             >  7:290618/1‑148 (MQ=255)
                                                                                ccAGCCCTGCTATCAAAACCCCTCAGAGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAAc                                                             >  6:16178/1‑148 (MQ=255)
                                                                                           aTCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCgg                                                 >  4:117365/1‑149 (MQ=255)
                                                                                            tCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCgg                                                 >  2:175380/1‑148 (MQ=255)
                                                                                                 atcccTCATGGCGTAGGATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTTGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTgg                                           <  8:290618/147‑1 (MQ=255)
                                                                                                 aCCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTCATGGAAGTTACTCCATTGGGCGCTTGTGATAACTATAACGCTCGGCATTgg                                           >  7:228574/1‑149 (MQ=255)
                                                                                                 aCCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTgg                                           <  4:127085/149‑1 (MQ=255)
                                                                                                             gTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATg                               <  6:222623/149‑1 (MQ=255)
                                                                                                             gTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATg                               >  1:118610/1‑149 (MQ=255)
                                                                                                                gCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGt                            >  2:75981/1‑149 (MQ=255)
                                                                                                                  aTGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTa                                                                                                       <  6:57473/72‑1 (MQ=255)
                                                                                                                     aaTGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCcgg                        <  5:16178/148‑1 (MQ=255)
                                                                                                                       tgatagaaGCCCAGCCCCGCGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCcggcgg                     >  1:124428/7‑149 (MQ=255)
                                                                                                                         atagaaGCCCAGCCCCGCGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGc                   >  1:246945/5‑149 (MQ=255)
                                                                                                                          tagaaGCCCAGCCCCGCGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGcc                  <  2:246945/146‑1 (MQ=255)
                                                                                                                                      gcgGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCTctgcct      <  4:69720/149‑1 (MQ=255)
                                                                                                                                          ggctgggctTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATctct          >  6:110389/1‑141 (MQ=255)
                                                                                                                                          ggctgggctTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATctct          <  5:110389/141‑1 (MQ=255)
                                                                                                                                          ggctgggctTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCTCTgcctgcct  <  8:93827/149‑1 (MQ=255)
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CACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCTCTGCCTGCCT  >  NC_002947/4301441‑4301727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑