Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 326,068 T→G 8.2% A555A (GCA→GCC)  PP_0269 ← glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947326,0680TG 8.2% 169.8 / 5.0 59A555A (GCA→GCC) PP_0269glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base T (29/25);  new base G (2/3);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

ATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCACCTGG  >  NC_002947/325921‑326214
                                                                                                                                                   |                                                                                                                                                  
aTCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATg                                                                                                                                                   >  6:11378/1‑149 (MQ=255)
    accTGAGTAGCAAGATCTTGCGTACCAAAAACTCCGTGCAGCAGAACGGTAACAACGACGACGGCAACTAACTCCGCGAATTCGTCGAGTACCCGATCACCAACGTCTGAGTATCATCTGATGTATCGAACAGCCCCGGCCTATCCCgg                                                                                                                                               >  8:223951/3‑149 (MQ=255)
       tGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGc                                                                                                                                            >  4:188061/1‑149 (MQ=255)
         cGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCt                                                                                                                                           <  4:293023/148‑1 (MQ=255)
         cGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCATAGGCCGGGGc                                                                                                                                            <  1:168824/147‑1 (MQ=255)
          gTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGt                                                                                                                                         >  8:74526/1‑149 (MQ=255)
             gCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTc                                                                                                                                      <  5:288566/149‑1 (MQ=255)
                    cTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCg                                                                                                                                <  3:188061/148‑1 (MQ=255)
                       gCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCg                                                                                                                             >  3:242179/1‑148 (MQ=255)
                           aCCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCt                                                                                                                                           >  8:79390/1‑130 (MQ=255)
                           aCCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCt                                                                                                                                           <  7:79390/130‑1 (MQ=255)
                           aCCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAACTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACgg                                                                                                                        >  1:24317/1‑149 (MQ=255)
                           aCCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAAAGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCAGATTCTGAACTGATGTATCGAACAGACCCGGCCTATGCCGGGGCTGTTTCAATTCTGAGAACgg                                                                                                                        >  7:40739/1‑149 (MQ=255)
                              aacaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTAGAGTACCCGATCAGCATATTCTGATTCTGATCTGATGTATCGAACATCCCCGCCGAATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTa                                                                                                                     >  4:82809/1‑149 (MQ=255)
                               acaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTAc                                                                                                                    >  4:130644/1‑149 (MQ=255)
                               acaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTAc                                                                                                                    >  3:58672/1‑149 (MQ=255)
                               acaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTAc                                                                                                                    >  6:264774/1‑149 (MQ=255)
                                 aacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTg                                                                                                                  >  1:192962/1‑149 (MQ=255)
                                    gCCGTGCAGCAGAACGGTTACAAAGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCTTACTGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGttt                                                                                                               <  3:82809/149‑1 (MQ=255)
                                               gAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTg                                                                                                                                          >  3:191638/1‑111 (MQ=255)
                                               gAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTg                                                                                                                                          <  4:191638/111‑1 (MQ=255)
                                               gAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCt                                                                                                    >  5:252587/1‑149 (MQ=255)
                                                 aCGTTTACATTGACGACGGCAACGAAGTCCGCTTATTCGTCTATTTCCGTATCAGCATATTCTGATTCTGTTCTGATGTATCTAACTGCCCCTGCCTATGGCGGTGCTGTTTCTTTTCGTCGAACGGTTACTGTTTTAGCCGGCGCTg                                                                                                   <  8:40739/148‑1 (MQ=255)
                                                 aCGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTgg                                                                                                  <  7:74526/149‑1 (MQ=255)
                                                     ttACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCt                                                                                               >  5:37566/1‑148 (MQ=255)
                                                       aCAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTAATGTTTGAGCCGGCGCTGGGCTTAc                                                                                            >  8:201091/1‑149 (MQ=255)
                                                                    cAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCa                                                                               <  6:37566/149‑1 (MQ=255)
                                                                      aCGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCg                                                                                                                                <  3:22658/98‑1 (MQ=255)
                                                                      aCGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCg                                                                                                                                >  4:22658/1‑98 (MQ=255)
                                                                         aaGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCt                                                                          <  5:68608/149‑1 (MQ=255)
                                                                                     tcgtcgAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTAcgcg                                                               <  2:24317/148‑1 (MQ=255)
                                                                                       gtcgAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGcc                                                             <  5:11378/148‑1 (MQ=255)
                                                                                       gtcgAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGGCTCCGGTGCAAAGCTGTCGCTACGCGCCa                                                            >  2:256553/1‑149 (MQ=255)
                                                                                        tcgAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCAt                                                           <  3:178781/149‑1 (MQ=255)
                                                                                            gTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGc                                                       <  4:58672/149‑1 (MQ=255)
                                                                                                  gATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAg                                                                            >  4:5437/1‑122 (MQ=255)
                                                                                                  gATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAg                                                                            <  3:5437/122‑1 (MQ=255)
                                                                                                       gCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCcgcg                                             >  6:197631/1‑148 (MQ=255)
                                                                                                        cATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCa                                           <  3:130644/149‑1 (MQ=255)
                                                                                                         aTCTTCTTATTCTGATCTGATGTATCGAACATCCGCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTTCGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCTCGCa                                           <  7:223951/148‑1 (MQ=255)
                                                                                                                  ttctgatCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGc                                 <  7:201091/149‑1 (MQ=255)
                                                                                                                      gatctgatGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtc                             >  4:190069/1‑149 (MQ=255)
                                                                                                                            atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCcac                                                    <  8:146343/120‑1 (MQ=255)
                                                                                                                            atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCcac                                                    >  7:146343/1‑120 (MQ=255)
                                                                                                                            atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgatc                         <  4:242179/147‑1 (MQ=255)
                                                                                                                            atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgat                          <  2:192962/146‑1 (MQ=255)
                                                                                                                            atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcga                           <  5:264774/145‑1 (MQ=255)
                                                                                                                              aaaagaaaCAGCCCCGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGc                     >  7:56498/7‑149 (MQ=255)
                                                                                                                              aaaagaaaCAGCCCCGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGc                     >  1:28160/7‑149 (MQ=255)
                                                                                                                               aaagaaaCAGCCCCGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGcc                    <  8:56498/144‑1 (MQ=255)
                                                                                                                                    aaCAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACt                 >  4:139067/1‑147 (MQ=255)
                                                                                                                                        gCCCCGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTaa           <  2:28160/149‑1 (MQ=255)
                                                                                                                                         ccccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCca                                                     <  6:199405/106‑1 (MQ=255)
                                                                                                                                         ccccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCca                                                     >  5:199405/1‑106 (MQ=255)
                                                                                                                                         ccccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCttt                              <  1:157578/129‑1 (MQ=255)
                                                                                                                                         ccccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCttt                              >  2:157578/1‑129 (MQ=255)
                                                                                                                                         ccccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAAc          >  1:236388/1‑149 (MQ=255)
                                                                                                                                                 tATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCACCTgg  >  1:108713/1‑149 (MQ=255)
                                                                                                                                                 tATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCACCTgg  >  1:105322/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCACCTGG  >  NC_002947/325921‑326214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑