Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,333,094 T→A 7.8% intergenic (+34/+50) PP_3804 → / ← yaiW hypothetical protein/lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,333,0940TA 7.8% 144.3 / 2.8 51intergenic (+34/+50)PP_3804/yaiWhypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (26/21);  new base A (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GTGATCAAGGCGGCCGGGGCCAAGCTGGTGGTGGTGGAGAGTGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAACCAGGCCGTGGTCAGCTTGCGGGTAATCTTCGGGCTCTT  >  NC_002947/4332947‑4333230
                                                                                                                                                   |                                                                                                                                        
gTGATCAAGGCGGCCGGGGCCAAGCTGGTGGTGGTGGAGAGTGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCtt                                                                                                                                          <  4:235588/148‑1 (MQ=255)
gTGATCAAGGCGGCCGGGGCCAAGCTGGTGGTGGTGGAGAGTGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTc                                                                                                                                         >  8:180783/1‑149 (MQ=255)
     cAAGGCGGCCGGGGCCAAGCTGGTGGTGGTGGAGAGTGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGccc                                                                                                                                     <  1:194907/148‑1 (MQ=255)
       aGGCGGCCGGGGCCAAGCTGGTGGTGGTGGAGAGTGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCgtg                                                                                                                                  >  7:52579/1‑149 (MQ=255)
       aGGCGGCCGGGGCCAAGCTGGTGGTGGTGGAGAGTGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCGAAGCCCgtg                                                                                                                                  <  1:15655/149‑1 (MQ=255)
       aGGCGGCCGGGGCCAAGCTGGTGGTGGTGGAGAGTGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCGAAGCCCgtg                                                                                                                                  <  7:170860/149‑1 (MQ=255)
       aGGCGGCCGGGGCCAAGCTGGTGGTGGTGGAGAGTGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCGAAGCCCgtg                                                                                                                                  >  2:15655/1‑149 (MQ=255)
              cGGGGCCAAGCTGGTGGTGGTGGAGAGTGACCCTGAAGATGCGATTGCGGGGCTTTTAGCCAGCTTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCgg                                                                                                                           <  1:98515/149‑1 (MQ=255)
               ggggCCAAGCTGGTGGTGGTGGAGAGTGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCggg                                                                                                                          >  2:212951/1‑149 (MQ=255)
                       gCTGGTGGTGGTGGAGAGTGTCCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCg                                                                                                                  >  7:216577/1‑149 (MQ=255)
                            tggtggtggAGAGTGTCCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCaa                                                                                                             <  8:216577/149‑1 (MQ=255)
                            tggtggtggAGAGTGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCAGTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCaa                                                                                                             <  1:196494/149‑1 (MQ=255)
                                 gtggAGAGTGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGAt                                                                                                         <  3:291440/148‑1 (MQ=255)
                                    gagaGTGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATcgcg                                                                                                     >  2:68106/1‑149 (MQ=255)
                                      gagTGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGt                                                                                                   <  5:94549/149‑1 (MQ=255)
                                         tGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTtgc                                                                                                >  3:257564/1‑149 (MQ=255)
                                         tGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTtgc                                                                                                >  8:48052/1‑149 (MQ=255)
                                             ccTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTtgctgc                                                                                             >  3:215201/1‑148 (MQ=255)
                                              cTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCa                                                                                           <  1:8994/149‑1 (MQ=255)
                                               tGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAg                                                                                          >  6:271501/1‑149 (MQ=255)
                                                       cGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGc                                                                                  >  8:153434/1‑149 (MQ=255)
                                                         aTTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTGGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCtt                                                                                <  1:68106/149‑1 (MQ=255)
                                                            gCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCa                                                                              <  3:60350/148‑1 (MQ=255)
                                                              ggggCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATcg                                                                                                       >  2:268309/1‑121 (MQ=255)
                                                              ggggCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATcg                                                                                                       <  1:268309/121‑1 (MQ=255)
                                                                  cTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCg                                                                       <  7:48052/149‑1 (MQ=255)
                                                                          cAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAgc                                                               >  3:210983/1‑149 (MQ=255)
                                                                          cAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAgc                                                               >  4:14691/1‑149 (MQ=255)
                                                                           aGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTGGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTACCGCTTCATGAACCGCTGATAgcg                                                              >  6:22632/1‑149 (MQ=255)
                                                                                 gACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATc                                                        >  5:289365/1‑149 (MQ=255)
                                                                                    cAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAAc                                                                                    >  4:270638/1‑118 (MQ=255)
                                                                                    cAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAAc                                                                                    <  3:270638/118‑1 (MQ=255)
                                                                                    cAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCAc                                                     >  4:30355/1‑149 (MQ=255)
                                                                                               ggCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCa                                           >  5:40594/1‑148 (MQ=255)
                                                                                                gCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCAc                                                     <  8:227892/137‑1 (MQ=255)
                                                                                                gCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCAc                                                     >  7:227892/1‑137 (MQ=255)
                                                                                                   ttGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAAc                                        >  2:212779/1‑147 (MQ=255)
                                                                                                          ggcgCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAACCAGGCCGTg                               <  1:212951/149‑1 (MQ=255)
                                                                                                          ggcgCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAACCAGGCCGTg                               <  4:210983/149‑1 (MQ=255)
                                                                                                          ggcgCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAACCAGGCCGTg                               <  4:257564/149‑1 (MQ=255)
                                                                                                           gcgcTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAACCAGGCCGTgg                              <  6:289365/149‑1 (MQ=255)
                                                                                                                 aTCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAACCAGGCCGTGGTCAg                          <  5:271501/147‑1 (MQ=255)
                                                                                                                  tCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAACCAGGCCGTGGTCAGCtt                       >  8:144406/1‑149 (MQ=255)
                                                                                                                      aCAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAAc                                        <  7:189553/128‑1 (MQ=255)
                                                                                                                      aCAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAAc                                        >  8:189553/1‑128 (MQ=255)
                                                                                                                          gCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAACCAGGCCGTGGTCAGCTTGCGGGTaa               >  2:236386/1‑149 (MQ=255)
                                                                                                                              gcgcgGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAACCAGGCCGTGGTCAGCTTGCGGGTAATCt            >  6:271107/1‑148 (MQ=255)
                                                                                                                                  ggTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAACCAGGCCGTGGTCAGCTTGCGGGTAATCTTCggg       <  7:180783/149‑1 (MQ=255)
                                                                                                                                   gTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAACCAGGCCGTGGTCAGCTTGCGGGTAATCTTCGGGc      <  8:52579/149‑1 (MQ=255)
                                                                                                                                    tGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCCCTCATCCACCCGCCCGGCAAACCAGGCCGTGGTCAGCTTGCGGGTAATCTTCGGGct     >  4:260749/1‑149 (MQ=255)
                                                                                                                                    ccgcgaacACGGGCGAAGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAACCAGGCCGTGGTCAGCTTGCGGGTAATCTTCGGGct     >  3:131229/8‑149 (MQ=255)
                                                                                                                                        tGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAACCAGGCCGTGGTCAGCTTGCGGGTAATCTTCGGGCTCtt  >  7:202952/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
GTGATCAAGGCGGCCGGGGCCAAGCTGGTGGTGGTGGAGAGTGACCCTGAAGATGCGATTGCGGGGCTCATAGCCAGCGTTGACCAAGTGGTTGGGGCATTGGGCGGGCGCTGATCGGACAGGCCCGCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGTAAACCCGCTCAAGGATCGCGGTTGCTGCCAGGCTAACGCTTCATGCACCGCTGATAGCGCTCATCCACCCGCCCGGCAAACCAGGCCGTGGTCAGCTTGCGGGTAATCTTCGGGCTCTT  >  NC_002947/4332947‑4333230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑