Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,363,965 T→C 11.1% intergenic (+40/‑34) adhP → / → PP_3840 alcohol dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,363,9650TC11.1% 116.8 / 5.1 47intergenic (+40/‑34)adhP/PP_3840alcohol dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/23);  new base C (2/3);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

GTGCCCAAGGTAACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTC  >  NC_002947/4363821‑4364104
                                                                                                                                                |                                                                                                                                           
gtgCCCAAGGTAACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCGGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGAt                                                                                                                                         >  4:177480/1‑149 (MQ=255)
 tgCCCAAGGTAACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGAt                                                                                                                                         >  6:130186/1‑148 (MQ=255)
   ctcAAGGTAACTCTGCTACCTATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGTCAAGATACAATGCCGGATGTTGAAACATTTCGAAGGATGACAGGCCGAGCCAGTTCATGTGGAACAACAGCATAGCCCGTATATGa                                                                                                                                       <  5:215871/146‑1 (MQ=255)
   cccAAGGTAACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGAc                                                                                                                                      >  7:187708/1‑149 (MQ=255)
              gCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGc                                                                                                                           >  4:239825/1‑149 (MQ=255)
                  cGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCtttt                                                                                                                       <  4:149890/149‑1 (MQ=255)
                  cGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCttt                                                                                                                        <  3:239825/148‑1 (MQ=255)
                  cGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGCAGAGGGTCATCTACGGGCtttg                                                                                                                       <  3:190504/149‑2 (MQ=255)
                  cGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGCAGAGGGTCATCTACGGGCtt                                                                                                                         <  5:53668/147‑1 (MQ=255)
                   gACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTa                                                                                                                      <  8:55195/149‑1 (MQ=255)
                   gACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGACGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTa                                                                                                                      >  3:206761/1‑149 (MQ=255)
                     ccGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGCAGAGGGTCATCTACGGGCtttaga                                                                                                                     >  1:107123/1‑145 (MQ=255)
                     ccGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGCAGAGGGTCATCTACGGGCtt                                                                                                                         <  2:107123/144‑1 (MQ=255)
                       gATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCct                                                                                                                  >  2:234658/1‑149 (MQ=255)
                         tCGAGGGTATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCcttc                                                                                                                <  4:271213/149‑1 (MQ=255)
                         tCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCcttc                                                                                                                <  6:247331/149‑1 (MQ=255)
                            aGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCcttctt                                                                                                              >  6:226106/1‑148 (MQ=255)
                               atatCAACCAGATCTTTGACGAGATGCTGTAGGGCAAGTTCAAAGGCCGGATGGTGATACAGTTCGACGGCTGACAGGCCGAGCCATGTCATGTGGAACCTCAGCAAAGCCCGTAGTTGACCCTCTGCGGGCTTTTAGCCTTCTTAAtt                                                                                                          <  8:192996/149‑1 (MQ=255)
                               atatCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAAtt                                                                                                          >  6:48940/1‑149 (MQ=255)
                               atatCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAAtt                                                                                                          <  8:187708/149‑1 (MQ=255)
                                  tCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTc                                                                                                       <  4:206761/149‑1 (MQ=255)
                                     aCCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCaaa                                                                                                    <  2:223249/149‑1 (MQ=255)
                                     aCCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCa                                                                                                      >  3:156680/1‑147 (MQ=255)
                                            cTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCct                                                                                                                                   <  5:86302/111‑1 (MQ=255)
                                            cTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCct                                                                                                                                   >  6:86302/1‑111 (MQ=255)
                                              ttGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACGGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGAc                                                                                           >  1:218009/1‑149 (MQ=255)
                                               tGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACa                                                                                              <  5:48940/145‑1 (MQ=255)
                                                 aCGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCa                                                                                         <  4:202108/148‑1 (MQ=255)
                                                         cTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTc                                                                                 <  1:80756/148‑1 (MQ=255)
                                                             aGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTg                                                                            <  4:184283/149‑1 (MQ=255)
                                                                        aaaGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTCCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGc                                                                 >  7:220235/1‑149 (MQ=255)
                                                                            gCCGGATGTTTATACAGTTCGAAGGCTGTCAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGAt                                                             <  8:220235/149‑1 (MQ=255)
                                                                                     tGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCAGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGACGACAGGCACGCCCGGCCGATTAGCCGCAc                                                    >  4:92376/1‑149 (MQ=255)
                                                                                             ttCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCTCAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCAc                                            <  5:269079/149‑1 (MQ=255)
                                                                                                   ggCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGAc                                       >  3:30393/1‑148 (MQ=255)
                                                                                                      tGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAg                                    >  7:79054/1‑148 (MQ=255)
                                                                                                            gCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTAcc                             <  5:226106/149‑1 (MQ=255)
                                                                                                            gCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTAcc                             >  8:184354/1‑149 (MQ=255)
                                                                                                            gCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTAcc                             <  2:218009/149‑1 (MQ=255)
                                                                                                               gAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCa                          <  8:79054/149‑1 (MQ=255)
                                                                                                                       tCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACg                     >  5:265822/1‑146 (MQ=255)
                                                                                                                                  cacaGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCa       <  3:92376/149‑1 (MQ=255)
                                                                                                                                      taaaaGCCCGAAGAGGGTCATCTACGGGCATTTACCCTTCTTAATTGTCAAAGGAACAGACCATGACCGACGACAGCCACGCCGGGCAGATTAGCCGAACTACAACACTGGACAACAATAACCGAAACAAGCAGAATGATCGTCACtt    >  8:131037/3‑148 (MQ=255)
                                                                                                                                       cAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTc  <  5:130186/149‑1 (MQ=255)
                                                                                                                                       aaaaGCCCGCAGAGGGTCATCTACGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTc  >  5:32998/2‑149 (MQ=255)
                                                                                                                                              cGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGttt   >  4:77767/1‑141 (MQ=255)
                                                                                                                                              cGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGttt   <  3:77767/141‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                           
GTGCCCAAGGTAACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTC  >  NC_002947/4363821‑4364104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑