Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,382,822 T→C 11.7% intergenic (‑33/+229) PP_3857 ← / ← PP_3858 hypothetical protein/tail fiber protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,382,8220TC11.7% 125.6 / 8.1 51intergenic (‑33/+229)PP_3857/PP_3858hypothetical protein/tail fiber protein
Reads supporting (aligned to +/- strand):  ref base T (22/23);  new base C (4/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

ACCGCCGTTGACATAGACATGCTGCAGCTCCGACGCGCTCTTGGATTGCATTGCCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAATGCTTCATGACGCTCATTCGTGAGCGG  >  NC_002947/4382685‑4382967
                                                                                                                                         |                                                                                                                                                 
acCGCCGTTGACATAGACATTCTGCAGCTCCGACGCGCTCTTGGATTGCATTGCCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTgg                                                                                                                                        <  7:124329/149‑1 (MQ=255)
acCGCCGTTGACATAGACATGCTGCAGCTCCGACGCGCTCTTGGATTGCATTGCCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTgg                                                                                                                                        >  1:233189/1‑149 (MQ=255)
acCGCCGTTGACATAGACATGCTGCAGCTCCGACGCGCTCTTGGATTGCATTGCCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCACGGCGGGttttt                                                                                                                                        <  1:130167/149‑3 (MQ=255)
acCGCCGTTGACATAGACATGCTGCAGCTCCGACGCGCTCTTGGATTGCATTGCCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCACGGCGGGttttt                                                                                                                                        <  1:123766/149‑3 (MQ=255)
 ccgccgTTGACATAGACATGCTGCAGCTCCGACGCGCTCTTGGATTGCATTGCCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTggg                                                                                                                                       >  4:22459/1‑149 (MQ=255)
            aTAGACATGCTGCAGCTCCGACGCGCTCTTGGATTGCATTGCCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGAttt                                                                                                                            <  7:222270/149‑1 (MQ=255)
               gACATGCTGCAGGTTCGACGCGCTCTTTGATTGCATTGCCGCTTCGGACAACAGGCCTTAATAGGCTTCCTAAGCTGAAATATCAGTCATTCAGTTTTCTCCAGGCATCTAAAAACCCGCCGTGGCGGGTTTGTGTCAAACGATTTGa                                                                                                                          <  3:118114/148‑1 (MQ=255)
               gACATGCTGCAGCTCCGACGCGCTCTTGGATTGCATTGCCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGa                                                                                                                          <  2:233189/148‑1 (MQ=255)
                aCATGCTGCAGCTCCGACGCGCTCTTGGATTGCATTGCCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGc                                                                                                                        <  6:262341/149‑1 (MQ=255)
                          gCTCCGACGCGCTCTTGGATTGCATTGCCGCTTCGGACAACAGGCCTGAATACGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTa                                                                                                              <  6:87762/149‑1 (MQ=255)
                                aCGCGCTCTTGGATTGCATTGCCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATC‑AAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGGCa                                                                                                       >  2:149826/1‑149 (MQ=255)
                                           gATTGCATTGCCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGTGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACgg                                                                                             <  8:102329/149‑1 (MQ=255)
                                            attgcattgcCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGc                                                                                            >  5:269548/1‑149 (MQ=255)
                                                cattgcCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCGGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGAtt                                                                                        >  1:14767/1‑149 (MQ=255)
                                                cattgcCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGAtt                                                                                        >  5:293561/1‑149 (MQ=255)
                                                  ttgcCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCGGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTgg                                                                                      <  2:14767/149‑1 (MQ=255)
                                                  ttgcCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTg                                                                                       >  5:225382/1‑148 (MQ=255)
                                                     ccGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTGACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATc                                                                                   <  5:74958/149‑1 (MQ=255)
                                                     ccGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGct                                                                                                   <  5:299314/133‑1 (MQ=255)
                                                     ccGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGct                                                                                                   >  6:299314/1‑133 (MQ=255)
                                                             gACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGca                                                                            >  3:200542/1‑148 (MQ=255)
                                                             gACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATCCAATCGccgtaga                                                                           <  3:22459/149‑6 (MQ=255)
                                                                     gCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATcggcg                                                                   >  7:11539/1‑149 (MQ=255)
                                                                           aTAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAg                                                              <  4:200542/148‑1 (MQ=255)
                                                                               gCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACgg                                                                                             >  2:157568/1‑113 (MQ=255)
                                                                               gCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACgg                                                                                             <  1:157568/113‑1 (MQ=255)
                                                                                           gAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGc                                              >  1:1448/1‑148 (MQ=255)
                                                                                              atatCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCaaaa                                          <  6:269548/149‑1 (MQ=255)
                                                                                                   aGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTca                                     >  2:82661/1‑149 (MQ=255)
                                                                                                   aGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTc                                      <  6:293561/148‑1 (MQ=255)
                                                                                                    gTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTcat                                    <  1:82661/149‑1 (MQ=255)
                                                                                                         tCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTca                                     <  1:235686/143‑1 (MQ=255)
                                                                                                         tCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTca                                     >  2:235686/1‑143 (MQ=255)
                                                                                                          cACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTcatccatc                               >  7:169604/1‑148 (MQ=255)
                                                                                                             ttttCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAATg                           >  4:245267/1‑149 (MQ=255)
                                                                                                                 ctcCAGGCATC‑AAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACGATTACGCAAAACGTCATCCATCAATGCTTc                       >  2:25420/1‑148 (MQ=255)
                                                                                                                 ctcCAGGCATC‑AAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACGATTACGCAAAACGTCATCCATCAATGCTTc                       <  1:25420/148‑1 (MQ=255)
                                                                                                                     aGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAATGCTTCATGa                   <  2:1448/149‑1 (MQ=255)
                                                                                                                         aTCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAATGCTTCATGACGCt               <  6:225382/149‑1 (MQ=255)
                                                                                                                           cAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAATGCTTCATGACGCTCa             >  4:284096/1‑149 (MQ=255)
                                                                                                                            aaaaaaCCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAAt                            <  5:64345/133‑1 (MQ=255)
                                                                                                                            aaaaaaCCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAAt                            >  6:64345/1‑133 (MQ=255)
                                                                                                                             ccaaaCCCGCCACGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCTCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAATGCTTCATGACGATCAtt           >  4:299207/3‑149 (MQ=255)
                                                                                                                             ccaaaCCCGCCACGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAATGCTTCATGACGCTCAtt           >  1:193178/3‑149 (MQ=255)
                                                                                                                              caaaCCCGCCACGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAATGCTTCATGACGCTCATTc          >  5:271744/2‑149 (MQ=255)
                                                                                                                              caaaCCCGCCACGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAATGCTTCATGACGCTCATTc          >  5:251086/2‑149 (MQ=255)
                                                                                                                                aaCCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAATGCTTCATGACGCTCATTCGt        >  8:234912/1‑149 (MQ=255)
                                                                                                                                   ccgccgTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAATGCTTCATGACGCTCATTCGTGAg     >  7:66907/1‑149 (MQ=255)
                                                                                                                                    cgccgTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAATGCTTCATGACGCTCATTCGTGAGc    >  2:183374/1‑149 (MQ=255)
                                                                                                                                     gccgTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCTTCAATGCTTCATGACGCTCATTCGTGAGCg   <  1:183374/149‑1 (MQ=255)
                                                                                                                                      ccgTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAATGCTTCATGACGCTCATTCGTGAGCgg  <  8:11539/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                 
ACCGCCGTTGACATAGACATGCTGCAGCTCCGACGCGCTCTTGGATTGCATTGCCGCTTCGGACAACAGGCCTGAATAGGCTTCCAAAGCTGAAATATCAGTCATTCACTTTTCTCCAGGCATCAAAAAACCCGCCGTGGCGGGTTTGGGTCAAACGATTTGAGCAGGCTCGTTACGCGTCACGCTCTACGGCGATTGGATCGCCAGCATCATCGGCGGCGAGCTGTTTACCATTACGCAAAACGTCATCCATCAATGCTTCATGACGCTCATTCGTGAGCGG  >  NC_002947/4382685‑4382967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑