| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,449,262 | A→C | 6.7% | E85A (GAG→GCG) | nicR → | HTH‑type transcriptional repressor NicR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,449,262 | 0 | A | C | 6.7% | 170.2 / 2.7 | 59 | E85A (GAG→GCG) | nicR | HTH‑type transcriptional repressor NicR |
| Reads supporting (aligned to +/- strand): ref base A (28/27); new base C (2/2); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
CCAGCAGCAGGCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACTGAGCATGGG > NC_002947/4449122‑4449406 | ccAGCAGCAGGCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAgc < 5:43208/143‑1 (MQ=255)ccAGCAGCAGGCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTg > 5:161794/1‑148 (MQ=255) gcagGCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGgcgc < 5:186077/149‑1 (MQ=255) agGCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGgcgcgc < 3:21940/149‑1 (MQ=255) agGCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGgcgcgc < 3:154014/149‑1 (MQ=255) ggCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGCGCGCCTTCAGGcgctcg > 3:269909/1‑149 (MQ=255) gCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGgcgcgc > 1:254951/1‑147 (MQ=255) cTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGCGCGCCTTCAGGCGCTCGacg < 4:269909/149‑3 (MQ=255) cTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGc > 6:50899/1‑149 (MQ=255) tGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCt > 8:192942/1‑149 (MQ=255) cGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTgg < 5:258491/149‑1 (MQ=255) cGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTg < 6:161794/148‑1 (MQ=255) gACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCATCCGTCGCGCGCCTTCAGGCGCTCGacgatg < 1:175223/149‑6 (MQ=255) gCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGc > 4:74336/1‑149 (MQ=255) aCCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGc < 7:192942/148‑1 (MQ=255) tCCAGTTCGTCACCCTGTGCGCACTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGCCGCGCGACCAGGTGAAGCTTTCGCCAGACCCg > 6:105680/1‑149 (MQ=255) gTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCg > 5:253932/1‑149 (MQ=255) ccTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGgcgc > 3:115692/1‑148 (MQ=255) tgCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAgg < 3:74336/148‑1 (MQ=255) tgCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGt > 1:116476/1‑149 (MQ=255) gcCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAgg > 8:68292/1‑145 (MQ=255) gcCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAgg < 7:68292/145‑1 (MQ=255) gcgcGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCAACGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCAtcgtc > 5:145836/1‑148 (MQ=255) aCGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCAcc < 2:116476/149‑1 (MQ=255) gCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCCCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGaa < 4:1124/149‑1 (MQ=255) ccAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAgc > 7:212677/1‑149 (MQ=255) aGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCAtc < 4:49522/129‑1 (MQ=255) aGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCAtc > 3:49522/1‑129 (MQ=255) tCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAgcggcgc > 8:18465/1‑149 (MQ=255) cccAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAgcggcgcg > 4:157014/1‑149 (MQ=255) cAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAgcggcgcggc > 6:138032/1‑149 (MQ=255) tGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGAt > 3:109704/1‑149 (MQ=255) tGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGAGCATGAAGGCGCGCGAGCTGGTGCAGCTTTCGCAAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGAt > 5:72429/1‑149 (MQ=255) gATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCAtcgtc > 6:35484/1‑115 (MQ=255) gATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCAtcgtc < 5:35484/115‑1 (MQ=255) aTCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGAt < 2:254951/147‑1 (MQ=255) tCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGc > 1:28255/1‑148 (MQ=255) tCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGc > 1:454/1‑148 (MQ=255) aaGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCaa < 2:454/148‑1 (MQ=255) aaGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCaa < 3:157014/148‑1 (MQ=255) aGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATg < 6:253932/149‑1 (MQ=255) gCCACCGCGGTGGACCAGGCAACCATCCGCGGCAA‑CGTCGAGCGCCTGAAGGCCCGCGACCTGGTTCAGCATTCGCCAGACACGGGCGACCCGCCCAACGTCATCGTCGAACACACCGAAAGCGCCGCGGCCTTGCTCGATGCAATGa > 2:283621/1‑148 (MQ=255) ccACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCAc < 8:164035/115‑1 (MQ=255) ccACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCAc > 7:164035/1‑115 (MQ=255) aCCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGTAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATccc < 2:62227/149‑1 (MQ=255) cgcgGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCt < 7:18465/149‑1 (MQ=255) ggACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGccc < 2:28255/149‑1 (MQ=255) gACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAg < 7:99748/87‑1 (MQ=255) aGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAg < 6:145836/149‑1 (MQ=255) gCATCCATCCTCGGGAT‑CGTCGAGCGCCTGAAGTCGCGCGAGCTGGTGCAGCTTTCGCCAGACGCGGGCGACCGGCGCAAGGTCATCGTCGAACTAACCGAAAGCGGAGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGAt < 6:72429/149‑1 (MQ=255) gCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGAt < 5:50899/149‑1 (MQ=255) aaCCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGc > 3:107712/1‑116 (MQ=255) aaCCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGc < 4:107712/116‑1 (MQ=255) ccATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGc < 4:115692/149‑1 (MQ=255) aTCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCGGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCg > 1:5688/1‑148 (MQ=255) gcgGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACTg > 7:286372/1‑149 (MQ=255) cgGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACt > 7:122382/1‑147 (MQ=255) cgtcgAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACTGAGCATgg > 2:194788/1‑149 (MQ=255) ctcgCGCGCCTTCAGGCGCTCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACTGAGCATggg > 4:141348/2‑149 (MQ=255) | CCAGCAGCAGGCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACTGAGCATGGG > NC_002947/4449122‑4449406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |