Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,449,277	G→T	8.7%	R90L (CGC→CTC)	nicR →	HTH‑type transcriptional repressor NicR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,449,277	0	G	T	8.7%	167.8 / 5.0	57	R90L (CGC→CTC)	nicR	HTH‑type transcriptional repressor NicR
Reads supporting (aligned to +/- strand):  ref base G (28/24);  new base T (2/3);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

AGGCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACTGAGCATGGGCAGCCTGAATGCCGGG  >  NC_002947/4449130‑4449422                                                                                                                                                     |                                                                                                                                                  agGCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGgcgcgc                                                                                                                                                  <  3:21940/149‑1 (MQ=255) agGCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGgcgcgc                                                                                                                                                  <  3:154014/149‑1 (MQ=255)  ggCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGCGCGCCTTCAGGcgctcg                                                                                                                                                 >  3:269909/1‑149 (MQ=255)   gCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGgcgcgc                                                                                                                                                  >  1:254951/1‑147 (MQ=255)     cTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGCGCGCCTTCAGGCGCTCGacg                                                                                                                                              <  4:269909/149‑3 (MQ=255)     cTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGc                                                                                                                                              >  6:50899/1‑149 (MQ=255)      tGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCt                                                                                                                                             >  8:192942/1‑149 (MQ=255)        cGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTgg                                                                                                                                           <  5:258491/149‑1 (MQ=255)        cGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTg                                                                                                                                            <  6:161794/148‑1 (MQ=255)         gACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCATCCGTCGCGCGCCTTCAGGCGCTCGacgatg                                                                                                                                           <  1:175223/149‑6 (MQ=255)              gCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGc                                                                                                                                     >  4:74336/1‑149 (MQ=255)                     aCCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGc                                                                                                                               <  7:192942/148‑1 (MQ=255)                            tCCAGTTCGTCACCCTGTGCGCACTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGCCGCGCGACCAGGTGAAGCTTTCGCCAGACCCg                                                                                                                       >  6:105680/1‑149 (MQ=255)                                gTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCg                                                                                                                   >  5:253932/1‑149 (MQ=255)                                         ccTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGgcgc                                                                                                           >  3:115692/1‑148 (MQ=255)                                             tgCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAgg                                                                                                       <  3:74336/148‑1 (MQ=255)                                             tgCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGt                                                                                                      >  1:116476/1‑149 (MQ=255)                                                gcCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAgg                                                                                                       <  7:68292/145‑1 (MQ=255)                                                gcCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAgg                                                                                                       >  8:68292/1‑145 (MQ=255)                                                     gcgcGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCAACGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCAtcgtc                                                                                               >  5:145836/1‑148 (MQ=255)                                                             aCGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCAcc                                                                                      <  2:116476/149‑1 (MQ=255)                                                                gCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCCCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGaa                                                                                   <  4:1124/149‑1 (MQ=255)                                                                   ccAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAgc                                                                                >  7:212677/1‑149 (MQ=255)                                                                     aGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCAtc                                                                                                  >  3:49522/1‑129 (MQ=255)                                                                     aGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCAtc                                                                                                  <  4:49522/129‑1 (MQ=255)                                                                        tCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAgcggcgc                                                                           >  8:18465/1‑149 (MQ=255)                                                                         cccAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAgcggcgcg                                                                          >  4:157014/1‑149 (MQ=255)                                                                           cAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAgcggcgcggc                                                                        >  6:138032/1‑149 (MQ=255)                                                                                     tGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGAt                                                              >  3:109704/1‑149 (MQ=255)                                                                                     tGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGAGCATGAAGGCGCGCGAGCTGGTGCAGCTTTCGCAAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGAt                                                              >  5:72429/1‑149 (MQ=255)                                                                                      gATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCAtcgtc                                                                                               <  5:35484/115‑1 (MQ=255)                                                                                      gATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCAtcgtc                                                                                               >  6:35484/1‑115 (MQ=255)                                                                                       aTCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGAt                                                              <  2:254951/147‑1 (MQ=255)                                                                                        tCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGc                                                            >  1:454/1‑148 (MQ=255)                                                                                        tCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGc                                                            >  1:28255/1‑148 (MQ=255)                                                                                          aaGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCaa                                                          <  3:157014/148‑1 (MQ=255)                                                                                          aaGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCaa                                                          <  2:454/148‑1 (MQ=255)                                                                                           aGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATg                                                        <  6:253932/149‑1 (MQ=255)                                                                                             gCCACCGCGGTGGACCAGGCAACCATCCGCGGCAA‑CGTCGAGCGCCTGAAGGCCCGCGACCTGGTTCAGCATTCGCCAGACACGGGCGACCCGCCCAACGTCATCGTCGAACACACCGAAAGCGCCGCGGCCTTGCTCGATGCAATGa                                                       >  2:283621/1‑148 (MQ=255)                                                                                              ccACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCAc                                                                                       <  8:164035/115‑1 (MQ=255)                                                                                              ccACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCAc                                                                                       >  7:164035/1‑115 (MQ=255)                                                                                                aCCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGTAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATccc                                                   <  2:62227/149‑1 (MQ=255)                                                                                                  cgcgGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCt                                                 <  7:18465/149‑1 (MQ=255)                                                                                                        ggACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGccc                                           <  2:28255/149‑1 (MQ=255)                                                                                                         gACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAg                                                                                                        <  7:99748/87‑1 (MQ=255)                                                                                                             aGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAg                                      <  6:145836/149‑1 (MQ=255)                                                                                                               gCATCCATCCTCGGGAT‑CGTCGAGCGCCTGAAGTCGCGCGAGCTGGTGCAGCTTTCGCCAGACGCGGGCGACCGGCGCAAGGTCATCGTCGAACTAACCGAAAGCGGAGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGAt                                    <  6:72429/149‑1 (MQ=255)                                                                                                               gCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGAt                                    <  5:50899/149‑1 (MQ=255)                                                                                                                 aaCCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGc                                                                   <  4:107712/116‑1 (MQ=255)                                                                                                                 aaCCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGc                                                                   >  3:107712/1‑116 (MQ=255)                                                                                                                   ccATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGc                                <  4:115692/149‑1 (MQ=255)                                                                                                                     aTCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCGGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCg                               >  1:5688/1‑148 (MQ=255)                                                                                                                         gcgGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACTg                          >  7:286372/1‑149 (MQ=255)                                                                                                                          cgGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACt                           >  7:122382/1‑147 (MQ=255)                                                                                                                                 cgtcgAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACTGAGCATgg                   >  2:194788/1‑149 (MQ=255)                                                                                                                                  ctcgCGCGCCTTCAGGCGCTCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACTGAGCATggg                  >  4:141348/2‑149 (MQ=255)                                                                                                                                       gcgcCTTCAGGCGCTCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGAt                                                      <  7:253608/108‑1 (MQ=255)                                                                                                                                       gcgcCTTCAGGCGCTCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGAt                                                      >  8:253608/1‑108 (MQ=255)                                                                                                                                          ccTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACTGAGCATGGGCAGCCTg           >  7:45501/1‑148 (MQ=255)                                                                                                                                                ggCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAAc                            >  3:68710/1‑125 (MQ=255)                                                                                                                                                ggCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAAc                            <  4:68710/125‑1 (MQ=255)                                                                                                                                                  cgcgcgAGCTGGTGCGGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACTGAGCATGGGCAGCCTGAATGCCggg  <  2:5688/149‑1 (MQ=255)                                                                                                                                                  cgcgcgAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACTGAGCATGGGCAGCCTGAATGCCggg  <  5:105680/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  AGGCCTGCGACCCGCAGCTTACCTCGATCCAGTTCGTCACCCTGTGCGCCCTGCGCGATCACGGCCCCAGCTCCCAGGCTGAACTGATCAAGGCCACCGCGGTGGACCAGGCAACCATCCGCGGCAT‑CGTCGAGCGCCTGAAGGCGCGCGAGCTGGTGCAGCTTTCGCCAGACCCGGGCGACCGGCGCAAGGTCATCGTCGAACTCACCGAAAGCGGCGCGGCCTTGCTCGATGCAATGATCCCCTGCGCCCGGCAGATCAGCGAACTGAGCATGGGCAGCCTGAATGCCGGG  >  NC_002947/4449130‑4449422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑