| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,525,551 | A→C | 6.3% | H161P (CAC→CCC) | mnmA → | tRNA 2‑thiouridine(34) synthase MnmA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,525,551 | 0 | A | C | 6.3% | 182.4 / 2.1 | 60 | H161P (CAC→CCC) | mnmA | tRNA 2‑thiouridine(34) synthase MnmA |
| Reads supporting (aligned to +/- strand): ref base A (27/29); new base C (2/2); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
CTCGACTACGCCCTGTCGCTGGGTGCCGACCTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGATTCCACCGGCATCTGCTTCATTGGCGA > NC_002947/4525421‑4525692 | cTCGACTACGCCCTGTCGCTGGGTGCCGACCTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACGCGAACAAGGACCAGAGCTACTTCCTGGCCGCCGACGGCGTGCAgg < 5:114330/148‑1 (MQ=255)cTCGACTACGCCCTGTCGCTGGGTGCCGACCTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAgg > 5:73252/1‑148 (MQ=255) gACTACGCCCTGTCGCTGGGTGCCGACCTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTCGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGaaa < 1:41896/148‑1 (MQ=255) ccTGTCGCTGGGTGCCGACCTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGca > 4:46367/1‑149 (MQ=255) tCGCTGGGTGCCGACCTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACt > 1:289867/1‑149 (MQ=255) gCTGGGTGCCGACCTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCg > 8:182470/1‑141 (MQ=255) gCTGGGTGCCGACCTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCg < 7:182470/141‑1 (MQ=255) ggTGCCGACCTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTccc < 6:73252/149‑1 (MQ=255) gTGCCGACCTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGGCCGCCGACGGCGTGCAGGaagta > 5:177384/1‑128 (MQ=255) gTGCCGACCTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGGCCGCCGACGGCGTGCAGGaagta < 6:177384/131‑4 (MQ=255) cGACCTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCg < 5:166178/149‑1 (MQ=255) cGACCTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCg < 2:198999/149‑1 (MQ=255) cTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCAGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCACGGTCGGCg > 5:260612/1‑148 (MQ=255) tGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGaa > 1:250488/1‑149 (MQ=255) gATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCACACACTGTTCCCGGTCGGCGAAc > 1:79096/1‑149 (MQ=255) cACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGaa > 8:149881/1‑148 (MQ=255) aCCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGaaaa < 8:193388/149‑1 (MQ=255) aCCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGaaaa < 4:252776/149‑1 (MQ=255) ggCCACTATGTGCGCCGCCGCGTTACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGcc > 7:193274/1‑149 (MQ=255) ccACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGccc > 8:49754/1‑148 (MQ=255) ccACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCg < 2:250488/149‑1 (MQ=255) aCTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGaa < 2:289867/149‑1 (MQ=255) cTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCACACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAg < 2:79096/149‑1 (MQ=255) tgCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTAcgc < 3:46367/149‑1 (MQ=255) gcgcCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGaa > 7:73862/1‑127 (MQ=255) gcgcCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGaa < 8:73862/127‑1 (MQ=255) cgccgccgcGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACgcgc < 7:149881/149‑1 (MQ=255) gccgcGTTACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATc < 8:193274/149‑1 (MQ=255) tGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGaaaaa > 4:40174/1‑148 (MQ=255) aacGAACTGCTCAATGGCCTGGACCCGAACAAGGACCAGAGCTACTACCTTCACGCCGTCGGCGGACAGGAAATCGCCCGCACACAGTTCCCGGTCGGCGAACTGGAAAAGCACGAAGTCCGCGACATCGCCGAAAAAGACGGCCTGGc > 8:162597/3‑149 (MQ=255) cGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGcc > 3:182576/1‑130 (MQ=255) cGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGcc < 4:182576/130‑1 (MQ=255) cGAACTGCTCAAGGGCCTGGACCCGAACAAGGACAAGAGCTACTTCATGCCCCCCGTCGGCGCCCCGGAAAACGCCCCCAAAATGTTCCCGGTCGGCGAAATGGAAATGCCCCAAGTACCCGCCTACGCGGAAAAACACGGCCTGGccc > 5:288787/1‑148 (MQ=255) cTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGcc > 6:176526/1‑147 (MQ=255) tCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACgg > 4:213611/1‑133 (MQ=255) tCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACgg < 3:213611/133‑1 (MQ=255) gggCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGc > 4:120525/1‑130 (MQ=255) gggCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGc < 3:120525/130‑1 (MQ=255) ggCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAgaagaa < 3:40174/149‑1 (MQ=255) ccTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCTCCATCTCCGAAAAACACGGCCTGGCCACCGCCAAGaagaaag < 5:176526/149‑1 (MQ=255) tGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGAt > 2:189018/1‑149 (MQ=255) ggACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCCCCGCCAAGaagcaagagt > 4:212935/1‑147 (MQ=255) ggACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGAtt > 6:122108/1‑149 (MQ=255) cccGAACAAGGACCAGAGATACTTCCTGCACGCCTTCGGCGTCCAGGAAATCTCCCGAACACTGTTCCCGGTCTGCGAACTGGAAAACCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGATTCCa < 7:162597/149‑1 (MQ=255) cctaaCAAGGACACTAGGAACTTCCTGCAGGCCGTCGGCGGCCTGGAAATCGCCCGCACACTGTCCCCGGGCGGCTAAATGCAAAAGCCCTAATAACCGGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGATTCCAc < 1:76459/146‑1 (MQ=255) ccGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGaagaaagagctcg > 5:256780/1‑143 (MQ=255) ccGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGaagaaa < 6:256780/141‑1 (MQ=255) gAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCAcc > 3:108629/1‑127 (MQ=255) gAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCAcc < 4:108629/127‑1 (MQ=255) gAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGATTCCAc > 4:110416/1‑147 (MQ=255) gAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGATTCCAc < 3:110416/147‑1 (MQ=255) gAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGATTCCACCg > 5:205105/1‑149 (MQ=255) aaGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGATTCCACCGGCa > 6:26905/1‑148 (MQ=255) ggACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGATTCCACCGGCATCt > 4:287005/1‑149 (MQ=255) ccAGAGCTACTTCCTGCACGCCGTCTGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGAt < 5:242390/133‑1 (MQ=255) ccAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACTGCCAAGAAGAAAGATTCCACCGGCATCTGCt < 1:189018/149‑1 (MQ=255) ccAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGAt > 6:242390/1‑133 (MQ=255) cTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGATTCCACCGGCATCTGCTTCATTg < 7:49754/149‑1 (MQ=255) cTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGATTCCACCGGCATCTGCTTCATTgg < 6:156118/147‑1 (MQ=255) ccTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGATTCCACCGGCATCTGCTTCATTGGCGa < 6:205105/147‑1 (MQ=255) | CTCGACTACGCCCTGTCGCTGGGTGCCGACCTGATCGCCACCGGCCACTATGTGCGCCGCCGCGATACTGGCGCGCTCACCGAACTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCCGTCGGCGGCCAGGAAATCGCCCGCACACTGTTCCCGGTCGGCGAACTGGAAAAGCCCGAAGTACGCGCCATCGCCGAAAAACACGGCCTGGCCACCGCCAAGAAGAAAGATTCCACCGGCATCTGCTTCATTGGCGA > NC_002947/4525421‑4525692 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |