Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,580,604 T→G 18.0% K942Q (AAG→CAG) ‡ treSB ← bifunctional trehalose synthase B/maltokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,580,6040TG18.0% 108.2 / 21.6 50K942Q (AAG→CAG) ‡treSBbifunctional trehalose synthase B/maltokinase
Reads supporting (aligned to +/- strand):  ref base T (23/18);  new base G (2/7);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

TAAGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGCACACCAG  >  NC_002947/4580456‑4580752
                                                                                                                                                    |                                                                                                                                                     
tAAGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTTCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCtgctt                                                                                                                                                       <  7:204598/149‑1 (MQ=255)
 aaGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCtgcttg                                                                                                                                                      >  1:55946/1‑149 (MQ=255)
      cTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATAGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAg                                                                                                                                                 >  6:194086/1‑149 (MQ=255)
        gtgtTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAggg                                                                                                                                               >  5:287646/1‑149 (MQ=255)
        gtgtTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCAGGCTCAggg                                                                                                                                               <  2:243041/149‑1 (MQ=255)
         tgtTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCAGGCTCAgg                                                                                                                                                <  2:75836/147‑1 (MQ=255)
         tgtTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGACACCCTGAGCAAGCAGGCTCAgg                                                                                                                                                <  7:244820/147‑1 (MQ=255)
           ttttGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGgtgt                                                                                                                                            >  3:138639/1‑149 (MQ=255)
           ttttGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCAGGCTCAGGgtgg                                                                                                                                            <  4:58585/149‑2 (MQ=255)
               gCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGa                                                                                                                                         <  3:107229/148‑1 (MQ=255)
                cccGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGACACCCTGAGCAAGCAGGCTCAGGgtggcctgc                                                                                                                                       <  2:215183/149‑7 (MQ=255)
                cccGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGAATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGAt                                                                                                                                        <  4:249214/148‑1 (MQ=255)
                 ccGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGAtgt                                                                                                                                      >  5:25919/1‑149 (MQ=255)
                  cGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGAtgtg                                                                                                                                     >  1:123436/1‑149 (MQ=255)
                     cgcTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGTTAGGCATAACCCTGCACCACGAGCAACTGGACCAGCTGCAGGTCGCAGTGTACAAGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTg                                                                                                                                  >  5:83953/1‑149 (MQ=255)
                            cAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTGGATGTGCTggg                                                                                                                                <  2:233675/144‑1 (MQ=255)
                            cAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAgg                                                                                                                           >  6:296285/1‑149 (MQ=255)
                            cAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCAGGCTCAgg                                                                                                                                                <  1:131109/128‑1 (MQ=255)
                            cAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCAGGCTCAgg                                                                                                                                                >  2:131109/1‑128 (MQ=255)
                              aGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGcc                                                                                                                         <  4:138639/149‑1 (MQ=255)
                                            ttCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCAGGCTCAgg                                                                                                                                                >  3:192858/1‑112 (MQ=255)
                                            ttCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCAGGCTCAgg                                                                                                                                                <  4:192858/112‑1 (MQ=255)
                                                ccTTCAAAATCGATGATGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGTGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAg                                                                                                      <  5:296285/149‑1 (MQ=255)
                                                  ttCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGgt                                                                                                    >  8:105721/1‑149 (MQ=255)
                                                              gAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAg                                                                                         <  2:55946/148‑1 (MQ=255)
                                                                       aTCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGa                                                                                              >  8:281061/1‑134 (MQ=255)
                                                                       aTCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGa                                                                                              <  7:281061/134‑1 (MQ=255)
                                                                                 accacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAg                                                                     >  1:143930/1‑149 (MQ=255)
                                                                                 accacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAg                                                                     >  1:203407/1‑149 (MQ=255)
                                                                                  ccacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAgt                                                                    <  5:194086/149‑1 (MQ=255)
                                                                                   cacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAgg                                                                                                                                                >  7:216566/1‑73 (MQ=255)
                                                                                   cacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAgg                                                                                                                                                <  8:216566/73‑1 (MQ=255)
                                                                                        gCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACt                                                                                     >  4:108317/1‑126 (MQ=255)
                                                                                        gCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACt                                                                                     <  3:108317/126‑1 (MQ=255)
                                                                                                cccAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTg                                                      <  7:105721/149‑1 (MQ=255)
                                                                                                          ggTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCcagc                                               <  6:139667/146‑1 (MQ=255)
                                                                                                          ggTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCcagc                                               >  5:139667/1‑146 (MQ=255)
                                                                                                            tCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTc                                          <  5:162732/149‑1 (MQ=255)
                                                                                                               ccGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAg                                       >  6:313059/1‑149 (MQ=255)
                                                                                                               ccGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGGCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAg                                       <  6:287646/149‑1 (MQ=255)
                                                                                                                    tACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGt                                  >  2:98244/1‑149 (MQ=255)
                                                                                                                      cacGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑GGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTgg                                <  5:313059/149‑1 (MQ=255)
                                                                                                                          cATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtca                            <  6:25919/149‑1 (MQ=255)
                                                                                                                          cATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGt                              >  4:34031/1‑147 (MQ=255)
                                                                                                                            tAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtcagt                          <  8:279999/149‑1 (MQ=255)
                                                                                                                               gCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtcagtt                         >  2:128751/1‑147 (MQ=255)
                                                                                                                               gCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtcagtt                         >  2:133443/1‑147 (MQ=255)
                                                                                                                               gCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtcagtt                         >  5:226585/1‑147 (MQ=255)
                                                                                                                                    ccACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGc                                                   >  1:278589/1‑116 (MQ=255)
                                                                                                                                    ccACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGc                                                   <  2:278589/116‑1 (MQ=255)
                                                                                                                                     cACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCt                  >  4:143402/1‑148 (MQ=255)
                                                                                                                                        ccTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTAGGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGAt               >  1:234624/1‑149 (MQ=255)
                                                                                                                                                   ttgatCAGGGTGTCGATGTGATGTGCCATTCCGTCACTCTGT‑TGCTGCAGGTCGTCGAAAAAGGACTGGCTATCGCGGTCCAGTGTGTCTCGATGCTGGGAAAGAAGGTCGAGGGAGTGTGTAAGTTCAGTGCTGATCTGTGCacac     >  6:89121/1‑147 (MQ=255)
                                                                                                                                                    tgctCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGCACACCAg  >  6:248290/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TAAGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGT‑TGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGCACACCAG  >  NC_002947/4580456‑4580752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑