Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,663,203	T→G	7.1%	A689A (GCT→GCG)	nuoG →	NADH‑quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,663,203	0	T	G	7.1%	198.0 / 2.3	64	A689A (GCT→GCG)	nuoG	NADH‑quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base T (27/32);  new base G (2/3);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCACGAAGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGTTCGCCTTCTCCATGGAAGGC  >  NC_002947/4663058‑4663344                                                                                                                                                  |                                                                                                                                               tCCACGAAGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCg                                                                                                                                             >  2:212935/1‑149 (MQ=255)  ccACGAAGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGt                                                                                                                                            >  1:265852/1‑149 (MQ=255)     cGAAGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTcc                                                                                                                                         <  2:265852/149‑1 (MQ=255)      gAAGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCg                                                                                                                                        >  2:47052/1‑149 (MQ=255)       aaGGCTGGCGCTGGATGCACGCTCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCAGCGCTCGGCGCGGCGttgacg                                                                                                                                       <  7:70596/149‑5 (MQ=255)               cgcTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAggg                                                                                                                               >  3:141860/1‑149 (MQ=255)               cgcTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAggg                                                                                                                               <  8:200919/149‑1 (MQ=255)                     aTGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGa                                                                                                                          >  6:202083/1‑148 (MQ=255)                      tGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAg                                                                                                                        >  5:266117/1‑149 (MQ=255)                         aCGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTg                                                                                                                     <  6:266117/149‑1 (MQ=255)                               tGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGCCCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCTTCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGt                                                                                                               >  4:186318/1‑149 (MQ=255)                                gCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGATGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGt                                                                                                               >  6:243417/1‑148 (MQ=255)                                 cGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGt                                                                                                               <  1:47052/147‑1 (MQ=255)                                 cGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTg                                                                                                              <  7:17120/148‑1 (MQ=255)                                 cGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGa                                                                                                             <  7:25635/149‑1 (MQ=255)                                   tGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGc                                                                                                           <  2:242838/149‑1 (MQ=255)                                      cACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGc                                                                                                                              <  1:299304/127‑1 (MQ=255)                                      cACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCGGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGc                                                                                                                              >  2:299304/1‑127 (MQ=255)                                        cccTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCTCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCt                                                                                                                      <  7:88867/133‑1 (MQ=255)                                        cccTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCt                                                                                                                      >  8:88867/1‑133 (MQ=255)                                         ccTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTgc                                                                                                    >  7:4361/1‑149 (MQ=255)                                         ccTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTgc                                                                                                    <  8:4361/149‑1 (MQ=255)                                             ctgAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTa                                                                                                <  3:257660/149‑1 (MQ=255)                                              tgAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTAc                                                                                               >  3:243337/1‑149 (MQ=255)                                                aaCAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTc                                                                                             >  1:87085/1‑149 (MQ=255)                                                aaCAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTc                                                                                             >  6:89484/1‑149 (MQ=255)                                                   aaGCCGGTCGACTGGACCCAGCTGGACCTCGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCgg                                                                                          <  8:146004/149‑1 (MQ=255)                                                          tCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTAcc                                                                                   <  5:89484/149‑1 (MQ=255)                                                           cGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCg                                                                                  >  2:2912/1‑149 (MQ=255)                                                                   cccAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGt                                                                          <  3:197031/149‑1 (MQ=255)                                                                      agcggtaCCCCGTCACCTGAGCCTGCGCTGAATCCGCTCGGCAAGTGGCTTGGATCGTGAAGGCCTCTGCGTGCGCTGGGTTCCGCATCAAGGGCATGAAGGTGGGCCGTGAGCCG‑CTTCGCTACTCCGGGCGTAGCGCCATGCGTGcc                                                                       <  8:202432/143‑1 (MQ=255)                                                                      accTGGACCACGTCCCCAGCTCCTGCGCCGAAGCCGCGCCGCAACTTGCTGGCATCGTCCACGCCGCGCCGAGCGCGGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGcc                                                                       <  8:206526/147‑1 (MQ=255)                                                                           gACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAAc                                                                    <  1:2912/147‑1 (MQ=255)                                                                            aCCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCg                                                                                           <  6:104931/123‑1 (MQ=255)                                                                               aCGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAgt                                                              >  6:13705/1‑149 (MQ=255)                                                                                 gTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAgtgt                                                            <  2:87085/149‑1 (MQ=255)                                                                                  tCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCGGCGTTCCGCAGCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCACCAGCAGtatg                                                           >  5:26683/1‑149 (MQ=255)                                                                                    aCCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGc                                                          <  4:141860/148‑1 (MQ=255)                                                                                      cAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCAc                                                        >  5:271529/1‑148 (MQ=255)                                                                                      cAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACg                                                       <  3:91211/149‑1 (MQ=255)                                                                                                          cTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGAc                                   <  6:271529/149‑1 (MQ=255)                                                                                                             cGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACaa                                 <  5:13705/148‑1 (MQ=255)                                                                                                              gCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCcg                                                  <  3:169147/130‑1 (MQ=255)                                                                                                              gCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCcg                                                  >  4:169147/1‑130 (MQ=255)                                                                                                               cAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGa                              <  1:212935/149‑1 (MQ=255)                                                                                                                aaCTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGAGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGa                              >  3:221926/1‑148 (MQ=255)                                                                                                                   tggctggcATCGTCAACGCCGCGCCGAGCGCTTCGTTCCGCATAAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCAGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAACCCCGAACACCGCAAGACAAGGACAcc                          >  1:219654/1‑149 (MQ=255)                                                                                                                          cATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCAGTCTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGTTc                    >  1:246159/1‑149 (MQ=255)                                                                                                                          cATCGACAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGTTc                    >  5:86545/1‑148 (MQ=255)                                                                                                                          cATCGACAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGTTc                    <  6:86545/148‑1 (MQ=255)                                                                                                                            tgcggaaCGCAGCGCTCGGCGCGGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGTTCGcc                 >  1:132074/6‑149 (MQ=255)                                                                                                                              gTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGc                                                                        <  7:198776/92‑1 (MQ=255)                                                                                                                              gTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGc                                                                        >  8:198776/1‑92 (MQ=255)                                                                                                                                 aaCGCCGCGCCGAGCGCTGTGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGTTCGCCTtctc            <  4:221926/149‑1 (MQ=255)                                                                                                                                    gccgcgccgAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGTTCGCCTTCTCCAt         <  4:243337/149‑1 (MQ=255)                                                                                                                                    gccgcgccgAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGTTCGCCTTCTCCAt         <  5:243417/149‑1 (MQ=255)                                                                                                                                     ccgcgccgAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGTTCGCCTTCTCCATg        >  4:9308/1‑149 (MQ=255)                                                                                                                                       gcgccgAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGt                      >  5:304114/1‑133 (MQ=255)                                                                                                                                       gcgccgAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGt                      <  6:304114/133‑1 (MQ=255)                                                                                                                                         gctcggcgcGGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGTTCGCCTTCTCCATGGAAg    <  2:132074/144‑1 (MQ=255)                                                                                                                                         gccgAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGTTCGCCTTCTCCATGGAAg    >  8:226420/1‑149 (MQ=255)                                                                                                                                           cgAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGTTCGCCTTCTCCATGGAAGGc  <  7:226420/149‑1 (MQ=255)                                                                                                                                               cgcTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGAc                                   >  2:58694/1‑112 (MQ=255)                                                                                                                                               cgcTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGAc                                   <  1:58694/112‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                               TCCACGAAGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCG‑CTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCGCACCCCGCAAGACAAGGACACCGCGTTCGCCTTCTCCATGGAAGGC  >  NC_002947/4663058‑4663344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑