Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,665,001 C→G 7.8% R45R (CGC→CGG)  nuoI → NADH‑quinone oxidoreductase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,665,0010CG 7.8% 193.4 / 5.0 61R45R (CGC→CGG) nuoINADH‑quinone oxidoreductase subunit I
Reads supporting (aligned to +/- strand):  ref base C (28/28);  new base G (2/3);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGATTTGACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAGTTCTTC  >  NC_002947/4664855‑4665148
                                                                                                                                                  |                                                                                                                                                   
aGGATTTGACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTa                                                                                                                                                   >  4:215593/1‑149 (MQ=255)
  gATTTGACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTAcc                                                                                                                                                 >  1:277195/1‑149 (MQ=255)
       gACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTCTACCTGCCGCCGCGCTACCGCGGc                                                                                                                                            >  2:73639/1‑149 (MQ=255)
         cccATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGcc                                                                                                                                           <  4:81104/148‑1 (MQ=255)
          ccATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCGGCCGCGGTAGCGCGGcgg                                                                                                                                          >  4:164313/1‑146 (MQ=255)
            aTGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCGGCCGCGGTAGCGCGGCGGCag                                                                                                                                       <  3:164313/149‑2 (MQ=255)
            aTGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGGTAGCGCGGCGGCag                                                                                                                                       <  8:76115/149‑2 (MQ=255)
                 cAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTc                                                                                                                                   <  1:125640/148‑1 (MQ=255)
                 cAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTc                                                                                                                                   <  7:70656/148‑1 (MQ=255)
                    gTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCa                                                                                                                               >  1:247302/1‑149 (MQ=255)
                                 aTCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGa                                                                                                                  >  6:86154/1‑149 (MQ=255)
                                  tCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGAt                                                                                                                 <  2:247302/149‑1 (MQ=255)
                                    gTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATgg                                                                                                               >  5:296984/1‑149 (MQ=255)
                                        aGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCga                                                                                                            <  2:277195/148‑1 (MQ=255)
                                        aGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCga                                                                                                            <  5:86154/148‑1 (MQ=255)
                                         gggCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACCCCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCCCCCGCGACCCCGATGGCgag                                                                                                           >  4:139581/1‑148 (MQ=255)
                                            caccggcaccCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAgc                                                                                                       >  6:139836/1‑149 (MQ=255)
                                                 gcaccCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGc                                                                                                  <  6:73781/149‑1 (MQ=255)
                                                        gCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGt                                                                                           <  6:180308/149‑1 (MQ=255)
                                                                    ggCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCCCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTgcg                                                                               >  2:178559/1‑149 (MQ=255)
                                                                     gCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTgc                                                                                <  3:139581/147‑1 (MQ=255)
                                                                       aaTGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCt                                                                                                    >  6:177502/1‑125 (MQ=255)
                                                                       aaTGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCt                                                                                                    <  5:177502/125‑1 (MQ=255)
                                                                       aaTGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTa                                                                                               <  6:16196/130‑1 (MQ=255)
                                                                        aTGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGt                                                                           <  6:296984/149‑1 (MQ=255)
                                                                                 tcCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGccc                                                                  >  5:105516/1‑149 (MQ=255)
                                                                                    cACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGt                                                               <  1:73639/149‑1 (MQ=255)
                                                                                    cACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCCCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGt                                                               <  1:178559/149‑1 (MQ=255)
                                                                                     aCGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTg                                                              >  8:250744/1‑149 (MQ=255)
                                                                                     aCGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTg                                                              >  4:12603/1‑149 (MQ=255)
                                                                                     aCGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCAGTCTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCCACACCCATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTACCCGGTGGCCTGCCCGGTg                                                              >  7:280494/1‑149 (MQ=255)
                                                                                        ggTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAAc                                                                                      >  3:264449/1‑122 (MQ=255)
                                                                                        ggTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAAc                                                                                      <  4:264449/122‑1 (MQ=255)
                                                                                         gTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCt                                                          >  5:50125/1‑149 (MQ=255)
                                                                                         gTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCt                                                          >  7:75303/1‑149 (MQ=255)
                                                                                             cGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCAt                                                      >  8:33760/1‑149 (MQ=255)
                                                                                             cGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCAt                                                      >  7:220424/1‑149 (MQ=255)
                                                                                               cAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCCCCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCAttt                                                    <  7:102221/149‑1 (MQ=255)
                                                                                                    gcgACACCCTGCAATAGCCCTAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTCGCGTGCAACATCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTg                                               <  7:152150/149‑1 (MQ=255)
                                                                                                    gcgACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTg                                               >  7:250934/1‑149 (MQ=255)
                                                                                                    gcgACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTg                                               >  2:3575/1‑149 (MQ=255)
                                                                                                     cgACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGc                                              <  8:75303/149‑1 (MQ=255)
                                                                                                     cgACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGc                                              <  3:215593/149‑1 (MQ=255)
                                                                                                       acacCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAg                                            <  4:195781/149‑1 (MQ=255)
                                                                                                             tGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGG‑CGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGc                                              >  6:148685/1‑140 (MQ=255)
                                                                                                             tGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGG‑CGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGc                                              <  5:148685/140‑1 (MQ=255)
                                                                                                               cAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCga                                    <  3:12603/149‑1 (MQ=255)
                                                                                                                    ccccGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACag                               <  5:139836/149‑1 (MQ=255)
                                                                                                                     cccGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACa                                >  3:10513/1‑147 (MQ=255)
                                                                                                                        gaagaaCCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGa                           >  4:125602/1‑149 (MQ=255)
                                                                                                                            aaCCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGTCGAGACAGAGGACGGc                       <  7:250744/149‑1 (MQ=255)
                                                                                                                             aCCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGcc                      >  8:122336/1‑149 (MQ=255)
                                                                                                                              cccGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCg                     >  7:266870/1‑149 (MQ=255)
                                                                                                                               ccGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACgg                        >  7:161413/1‑145 (MQ=255)
                                                                                                                               ccGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACgg                        <  8:161413/145‑1 (MQ=255)
                                                                                                                               ccGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGc                    >  4:21147/1‑149 (MQ=255)
                                                                                                                                      ccTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTAc              <  1:3575/148‑1 (MQ=255)
                                                                                                                                          ccgccgCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCg           <  6:105516/147‑1 (MQ=255)
                                                                                                                                           ggccgcGGTAGCGCGGCGGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTc                                                   <  1:230317/105‑1 (MQ=255)
                                                                                                                                           ggccgcGGTAGCGCGGCGGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTc                                                   >  2:230317/2‑106 (MQ=255)
                                                                                                                                           cgccgcGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAg        <  8:250934/149‑1 (MQ=255)
                                                                                                                                                 gcTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAGttcttc  <  7:33760/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGGATTTGACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAGTTCTTC  >  NC_002947/4664855‑4665148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑