Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,665,004 C→G 8.3% Y46* (TAC→TAG)  nuoI → NADH‑quinone oxidoreductase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,665,0040CG 8.3% 187.5 / 7.1 60Y46* (TAC→TAG) nuoINADH‑quinone oxidoreductase subunit I
Reads supporting (aligned to +/- strand):  ref base C (26/29);  new base G (2/3);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

GATTTGACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAGTTCTTCCGC  >  NC_002947/4664857‑4665151
                                                                                                                                                   |                                                                                                                                                   
gATTTGACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTAcc                                                                                                                                                    >  1:277195/1‑149 (MQ=255)
     gACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTCTACCTGCCGCCGCGCTACCGCGGc                                                                                                                                               >  2:73639/1‑149 (MQ=255)
       cccATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGcc                                                                                                                                              <  4:81104/148‑1 (MQ=255)
        ccATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCGGCCGCGGTAGCGCGGcgg                                                                                                                                             >  4:164313/1‑146 (MQ=255)
          aTGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCGGCCGCGGTAGCGCGGCGGCag                                                                                                                                          <  3:164313/149‑2 (MQ=255)
          aTGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGGTAGCGCGGCGGCag                                                                                                                                          <  8:76115/149‑2 (MQ=255)
               cAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTc                                                                                                                                      <  7:70656/148‑1 (MQ=255)
               cAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTc                                                                                                                                      <  1:125640/148‑1 (MQ=255)
                  gTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCa                                                                                                                                  >  1:247302/1‑149 (MQ=255)
                               aTCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGa                                                                                                                     >  6:86154/1‑149 (MQ=255)
                                tCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGAt                                                                                                                    <  2:247302/149‑1 (MQ=255)
                                  gTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATgg                                                                                                                  >  5:296984/1‑149 (MQ=255)
                                      aGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCga                                                                                                               <  2:277195/148‑1 (MQ=255)
                                      aGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCga                                                                                                               <  5:86154/148‑1 (MQ=255)
                                       gggCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACCCCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCCCCCGCGACCCCGATGGCgag                                                                                                              >  4:139581/1‑148 (MQ=255)
                                          caccggcaccCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAgc                                                                                                          >  6:139836/1‑149 (MQ=255)
                                               gcaccCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGc                                                                                                     <  6:73781/149‑1 (MQ=255)
                                                      gCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGt                                                                                              <  6:180308/149‑1 (MQ=255)
                                                                  ggCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCCCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTgcg                                                                                  >  2:178559/1‑149 (MQ=255)
                                                                   gCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTgc                                                                                   <  3:139581/147‑1 (MQ=255)
                                                                     aaTGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCt                                                                                                       >  6:177502/1‑125 (MQ=255)
                                                                     aaTGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCt                                                                                                       <  5:177502/125‑1 (MQ=255)
                                                                     aaTGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTa                                                                                                  <  6:16196/130‑1 (MQ=255)
                                                                      aTGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGt                                                                              <  6:296984/149‑1 (MQ=255)
                                                                               tcCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGccc                                                                     >  5:105516/1‑149 (MQ=255)
                                                                                  cACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGt                                                                  <  1:73639/149‑1 (MQ=255)
                                                                                  cACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCCCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGt                                                                  <  1:178559/149‑1 (MQ=255)
                                                                                   aCGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTg                                                                 >  4:12603/1‑149 (MQ=255)
                                                                                   aCGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTg                                                                 >  8:250744/1‑149 (MQ=255)
                                                                                   aCGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCAGTCTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCCACACCCATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTACCCGGTGGCCTGCCCGGTg                                                                 >  7:280494/1‑149 (MQ=255)
                                                                                      ggTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAAc                                                                                         <  4:264449/122‑1 (MQ=255)
                                                                                      ggTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAAc                                                                                         >  3:264449/1‑122 (MQ=255)
                                                                                       gTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCt                                                             >  7:75303/1‑149 (MQ=255)
                                                                                       gTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCt                                                             >  5:50125/1‑149 (MQ=255)
                                                                                           cGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCAt                                                         >  8:33760/1‑149 (MQ=255)
                                                                                           cGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCAt                                                         >  7:220424/1‑149 (MQ=255)
                                                                                             cAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCCCCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCAttt                                                       <  7:102221/149‑1 (MQ=255)
                                                                                                  gcgACACCCTGCAATAGCCCTAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTCGCGTGCAACATCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTg                                                  <  7:152150/149‑1 (MQ=255)
                                                                                                  gcgACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTg                                                  >  7:250934/1‑149 (MQ=255)
                                                                                                  gcgACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTg                                                  >  2:3575/1‑149 (MQ=255)
                                                                                                   cgACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGc                                                 <  8:75303/149‑1 (MQ=255)
                                                                                                   cgACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGc                                                 <  3:215593/149‑1 (MQ=255)
                                                                                                     acacCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAg                                               <  4:195781/149‑1 (MQ=255)
                                                                                                           tGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGG‑CGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGc                                                 <  5:148685/140‑1 (MQ=255)
                                                                                                           tGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGG‑CGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGc                                                 >  6:148685/1‑140 (MQ=255)
                                                                                                             cAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCga                                       <  3:12603/149‑1 (MQ=255)
                                                                                                                  ccccGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACag                                  <  5:139836/149‑1 (MQ=255)
                                                                                                                   cccGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACa                                   >  3:10513/1‑147 (MQ=255)
                                                                                                                      gaagaaCCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGa                              >  4:125602/1‑149 (MQ=255)
                                                                                                                          aaCCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGTCGAGACAGAGGACGGc                          <  7:250744/149‑1 (MQ=255)
                                                                                                                           aCCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGcc                         >  8:122336/1‑149 (MQ=255)
                                                                                                                            cccGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCg                        >  7:266870/1‑149 (MQ=255)
                                                                                                                             ccGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACgg                           >  7:161413/1‑145 (MQ=255)
                                                                                                                             ccGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACgg                           <  8:161413/145‑1 (MQ=255)
                                                                                                                             ccGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGc                       >  4:21147/1‑149 (MQ=255)
                                                                                                                                    ccTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTAc                 <  1:3575/148‑1 (MQ=255)
                                                                                                                                        ccgccgCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCg              <  6:105516/147‑1 (MQ=255)
                                                                                                                                         ggccgcGGTAGCGCGGCGGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTc                                                      <  1:230317/105‑1 (MQ=255)
                                                                                                                                         ggccgcGGTAGCGCGGCGGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTc                                                      >  2:230317/2‑106 (MQ=255)
                                                                                                                                         cgccgcGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAg           <  8:250934/149‑1 (MQ=255)
                                                                                                                                               gcTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAGttcttc     <  7:33760/149‑1 (MQ=255)
                                                                                                                                                   ccGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAGTTCTTCCGc  <  3:21147/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GATTTGACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAGTTCTTCCGC  >  NC_002947/4664857‑4665151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑