Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,674,591 C→T 7.3% R455C (CGC→TGC)  PP_4137 → outer membrane siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,674,5910CT 7.3% 159.2 / 2.8 55R455C (CGC→TGC) PP_4137outer membrane siderophore receptor
Reads supporting (aligned to +/- strand):  ref base C (26/25);  new base T (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

TCTACACGCGCCGCGAAAGTGACGTGACCTACCCCTCTGAAGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTGGGCGCCCGTGGCGACTCGGCGCTCGGCCGCTGG  >  NC_002947/4674454‑4674737
                                                                                                                                         |                                                                                                                                                  
tCTACACGCGCCGCGAAAGTGACGTGACCTACCCCTCTGAAGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTagag                                                                                                                                         >  4:124207/1‑149 (MQ=255)
     acGCGCCGCGAAAGTGACGTGACCTACCCCTCTGAAGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAgccgc                                                                                                                                     >  3:129224/1‑148 (MQ=255)
       gcgcCGCGAAAGTGACGTGACCTACCCCTCTGAAGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCTGCTCGGTAGAGCCGCca                                                                                                                                   >  4:77409/1‑148 (MQ=255)
       gcgcCGCGAAAGTGACGTGACCTACCCCTCTGAAGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCTGCTCGGTAGAGCCGCca                                                                                                                                   >  4:81611/1‑148 (MQ=255)
            gcgAAAGTGACGTGACCTACCCCTCTGAAGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCg                                                                                                                             >  8:168821/1‑149 (MQ=255)
            gcgAAAGTGACGTGACCTACCCCTCTGAAGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCg                                                                                                                             >  6:26430/1‑149 (MQ=255)
                          acCTACCCCTCTGAAGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTg                                                                                                               >  5:294255/1‑149 (MQ=255)
                            cTACCCCTCTGAAGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGa                                                                                                             <  4:129224/149‑1 (MQ=255)
                               ccccTCTGAAGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAgcg                                                                                                          >  3:190913/1‑149 (MQ=255)
                               ccccTCTGAAGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAg                                                                                                            <  5:26430/147‑1 (MQ=255)
                                       aaGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGccc                                                                                                     <  3:124207/146‑1 (MQ=255)
                                       aaGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCa                                                                                                    <  5:143496/147‑1 (MQ=255)
                                       aaGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAg                                                                                                   <  4:204342/148‑1 (MQ=255)
                                           cggcAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGcccagcccag                                                                                              >  2:99609/1‑149 (MQ=255)
                                            ggcAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAgg                                                                                             >  2:204044/1‑149 (MQ=255)
                                               aaGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAggcgg                                                                                          <  6:294255/149‑1 (MQ=255)
                                                     aGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAg                                                                                      >  4:1147/1‑147 (MQ=255)
                                                     aGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCa                                                                                    >  4:5422/1‑149 (MQ=255)
                                                        atgcatgACTGGGACTATGTCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGAt                                                                                 <  5:200690/149‑1 (MQ=255)
                                                        atgcatgACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTag                                                                                                                                           >  6:52501/1‑91 (MQ=255)
                                                           catgACTGGGACTATG‑CCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAg                                                                             <  2:134579/149‑1 (MQ=255)
                                                             tgACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGc                                                                            <  1:204044/149‑1 (MQ=255)
                                                             tgACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGc                                                                            <  3:5422/149‑1 (MQ=255)
                                                             tgACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGc                                                                            <  3:1147/149‑1 (MQ=255)
                                                                cTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGa                                                                         >  6:17237/1‑149 (MQ=255)
                                                                cTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGa                                                                         <  7:160439/149‑1 (MQ=255)
                                                                   ggACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCTGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCg                                                                      <  3:81611/149‑1 (MQ=255)
                                                                   ggACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCTGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCg                                                                      <  3:77409/149‑1 (MQ=255)
                                                                   ggACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGa                                                                                                                   >  7:116135/1‑104 (MQ=255)
                                                                   ggACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGa                                                                                                                   <  8:116135/104‑1 (MQ=255)
                                                                    gACTATGCTCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATc                                                                       >  2:255324/1‑147 (MQ=255)
                                                                            cccccGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAg                                                              >  2:224408/1‑148 (MQ=255)
                                                                            cccccGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAg                                                              >  1:294576/1‑148 (MQ=255)
                                                                                  ccTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGa                                                       >  8:111456/1‑149 (MQ=255)
                                                                                           gcgcTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGccacca                                              >  4:80365/1‑149 (MQ=255)
                                                                                             gcTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCaccacc                                            <  2:294576/149‑1 (MQ=255)
                                                                                                     aTCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCca                                                                                                                                   <  3:284494/54‑1 (MQ=255)
                                                                                                     aTCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCca                                                                                                                                   >  4:284494/1‑54 (MQ=255)
                                                                                                        cGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTggg                                 >  6:171570/1‑149 (MQ=255)
                                                                                                        cGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTgg                                  <  1:224408/148‑1 (MQ=255)
                                                                                                          cccGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGcca                                                 <  2:244649/131‑1 (MQ=255)
                                                                                                           ccGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTGGgcgc                              <  4:190913/149‑1 (MQ=255)
                                                                                                                   gCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTg                                         <  2:87306/130‑1 (MQ=255)
                                                                                                                   gCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTg                                         >  1:87306/1‑130 (MQ=255)
                                                                                                                     aGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTGGGCGCCCGTGGCGAc                    >  2:308759/1‑149 (MQ=255)
                                                                                                                             ggCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTGGGCGCCCGTGGCGACTCGGCGCt            >  8:260558/1‑149 (MQ=255)
                                                                                                                              gCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCaccacc                                            <  4:272162/116‑1 (MQ=255)
                                                                                                                              gCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCaccacc                                            >  3:272162/1‑116 (MQ=255)
                                                                                                                                   cTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTGGGCGCCCGTGGCGACTCGGCGCTCGGCCg      <  8:94579/149‑1 (MQ=255)
                                                                                                                                    tGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTGGGCGCCCGTGGCGACTCGGCGCTCGGCCGc     >  1:148058/1‑149 (MQ=255)
                                                                                                                                     gAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTGGGCGCCCGTg                         >  7:233311/1‑128 (MQ=255)
                                                                                                                                     gAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTGGGCGCCCGTg                         <  8:233311/128‑1 (MQ=255)
                                                                                                                                      aGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTGGGCGCCCGTGGCGACTCGGCGCTCGGCCGCt    <  3:80365/148‑1 (MQ=255)
                                                                                                                                      aGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTGGGCGCCCGTGGCGACTCGGCGCTCGGCCGCTg   <  1:255324/149‑1 (MQ=255)
                                                                                                                                       gCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTGGGCGCCCGTGGCGACTCGGCGCTCGGCCGCTgg  <  7:111456/149‑1 (MQ=255)
                                                                                                                                        ccGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTGGGCGCCCGTGGCGACTCGGCGCTCGGCCGCTgg  >  7:198320/1‑148 (MQ=255)
                                                                                                                                         |                                                                                                                                                  
TCTACACGCGCCGCGAAAGTGACGTGACCTACCCCTCTGAAGGCGGCAAGGTGAGCATGCATGACTGGGACTATGCCCCCCGCCTGGGGCTGCGCTACGACATCCGCCCGGACTTGCAGGTGTACGGCAACCTGAGCCGCTCGGTAGAGCCGCCACACCCGTGGTCGCTGATCTGGAGCGCGCCCAGCACAGGCGGCAAGCAGATCCAGCCGATCGAGATGCAGAACCAGACAGCCACCACCCTGGAACTGGGCGCCCGTGGCGACTCGGCGCTCGGCCGCTGG  >  NC_002947/4674454‑4674737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑