Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,740,839 C→G 9.5% intergenic (+76/+677) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8390CG 9.5% 175.5 / 9.9 63intergenic (+76/+677)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (31/26);  new base G (2/4);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01

GATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGT  >  NC_002947/4740691‑4740982
                                                                                                                                                    |                                                                                                                                                
gATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc                                                                                                                                                 <  4:204284/149‑1 (MQ=255)
     gCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGcc                                                                                                                                            <  6:182323/149‑1 (MQ=255)
       cGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcg                                                                                                                                          >  2:19101/1‑149 (MQ=255)
        gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcgc                                                                                                                                         <  7:139944/149‑1 (MQ=255)
           cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                                                      <  6:42544/149‑1 (MQ=255)
            gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAag                                                                                                                                     <  6:172930/149‑1 (MQ=255)
            gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAag                                                                                                                                     <  2:280994/149‑1 (MQ=255)
              gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                                                   <  6:128301/149‑1 (MQ=255)
              gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGACTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                                                                   <  8:53762/149‑1 (MQ=38)
                cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAAGAGGc                                                                                                                                 <  1:203867/149‑1 (MQ=38)
                 aGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGcc                                                                                                                                <  4:281514/149‑1 (MQ=255)
                  gcgttACACCGGCGAGCAGACGCGCGACATCGTTCCCCTCAAACACCGCACTTACGCTGCAGTCGCCCAACCCAAGACGGCGGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCg                                                                                                                               <  2:107039/145‑1 (MQ=255)
                  gCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCg                                                                                                                               <  6:277644/149‑1 (MQ=255)
                    tGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGGGAAGAGGCCgg                                                                                                                              >  7:164570/1‑148 (MQ=255)
                    tGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGGAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCgcc                                                                                                                             >  8:224237/1‑147 (MQ=255)
                               gAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGc                                                                                                                                             >  5:169463/1‑122 (MQ=255)
                               gAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGc                                                                                                                                             <  6:169463/122‑1 (MQ=255)
                                     aaGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAcc                                                                                                            >  7:38891/1‑149 (MQ=255)
                                       gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCt                                                                                                           >  7:67290/1‑148 (MQ=255)
                                           gACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACCGCCCCCACAAAACCTC‑AGtt                                                                                                     >  6:289837/1‑149 (MQ=255)
                                              aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCGC‑AGttttt                                                                                                  >  3:249776/1‑149 (MQ=255)
                                                 gTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTtctc                                                                                               >  1:42230/1‑149 (MQ=255)
                                                      ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCGTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTccgccc                                                                                          <  7:91394/149‑1 (MQ=255)
                                                       cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGCCACCACAAACCCGC‑AGTTTTTCTccgcccg                                                                                         >  7:207791/1‑149 (MQ=255)
                                                          aaaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCt                                                                                       >  3:71164/1‑148 (MQ=255)
                                                               ccGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACg                                                                                   >  1:210407/1‑147 (MQ=255)
                                                                cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAACTC‑AGTTTTTATCCGCCCGCGCAAGCAg                                                                                >  5:188577/1‑149 (MQ=255)
                                                                  cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGcc                                                                              >  7:209779/1‑149 (MQ=255)
                                                                         gCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                                                       <  1:19101/149‑1 (MQ=255)
                                                                         ccggCAGTACCCGACCGCACCACTGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCGGTGCCGGCCGCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                                                       <  4:249776/146‑1 (MQ=255)
                                                                          cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                                                      >  7:6941/1‑149 (MQ=255)
                                                                          cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                                                      <  4:71164/149‑1 (MQ=255)
                                                                              aGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt                                                                  <  2:42230/149‑1 (MQ=255)
                                                                               gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAtt                                                                 <  8:209779/149‑1 (MQ=255)
                                                                                      aaCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgc                                                          <  2:210407/149‑1 (MQ=255)
                                                                                       aCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgcg                                                         <  8:155615/149‑1 (MQ=255)
                                                                                            aaaagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTa                                                                   <  1:5595/132‑1 (MQ=255)
                                                                                            aaaagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTa                                                                   >  2:5595/3‑134 (MQ=38)
                                                                                             gaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATCGCCGCCATGCCCGCGAAGCGGCCGGCCCAGACCCCACAAAACCTC‑AGTTTTTCACCGCCCGCGAACGCACCCCTGTCAGGGTAATGAACAGGGCAAcca                                                   >  6:245792/1‑149 (MQ=255)
                                                                                                 gCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCcaccac                                               <  8:67290/149‑1 (MQ=255)
                                                                                                   tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTATTCGCGGGCTTGGGCGCGAAGAGGCCGGCACACACACCCCAAAACCGC‑AGATTTTCTCCGCACCGACACCCAGCCCCTTACCGGTATTGACCGGGGCGTCCACCACg                                              >  8:145536/1‑148 (MQ=255)
                                                                                                        tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGGGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttg                                        >  6:240051/1‑149 (MQ=255)
                                                                                                             cAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGc                                   >  7:10297/1‑149 (MQ=255)
                                                                                                               agccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGc                                   >  3:278097/5‑148 (MQ=255)
                                                                                                                 cTTTTTTTATTTGTCGTGTCCCGGCCGCTGGGCGCCCTGCCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccac                                <  6:188577/149‑1 (MQ=255)
                                                                                                                     ttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCCCAGACACCACAAAACCTC‑AGTTTTTGTACGCCCGCTAACGCAGCCCTTTAAGGGTATTGAACTGCGCAGACAAGACGAACTTGTTGGCCACCATc                            >  6:74141/1‑149 (MQ=255)
                                                                                                                                 tgTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACGTC‑AGGTTTTCTCCGCCCGCTCCCGCAGCCCGTTCAGGGTATTGAACGGCGCATCCCCCACGAACTTGTTGGCCACCATCGCCGGCACGCTa                >  5:154943/2‑149 (MQ=255)
                                                                                                                                   tGCCGGCCTCTTAGCGGCCTTTCCCGCGAAGCGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTGTACGCCCCCGCACGCAGCCCGTTGAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTac               >  6:68768/1‑148 (MQ=255)
                                                                                                                                      cGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCTTCGGCGGCACGCTaccacc           <  8:164570/149‑1 (MQ=255)
                                                                                                                                       ggCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCt          >  5:126551/1‑149 (MQ=255)
                                                                                                                                        gCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCCCGCACTTGTTGGCCACCATCGCCGGCACGCTACCCCCTg         >  7:217217/1‑149 (MQ=255)
                                                                                                                                          ctctTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTTTTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTgg        >  1:230194/1‑149 (MQ=255)
                                                                                                                                           tctTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCt      >  5:63002/1‑149 (MQ=255)
                                                                                                                                           tctTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCt      >  3:214914/1‑149 (MQ=255)
                                                                                                                                           tctTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCt      >  3:122330/1‑149 (MQ=255)
                                                                                                                                             ttCGCGGGCAAGGCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg                                     <  5:10391/116‑1 (MQ=39)
                                                                                                                                             ttCGCGGGCAAGGCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg                                     >  6:10391/1‑116 (MQ=39)
                                                                                                                                             ttCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCg    >  2:132217/1‑149 (MQ=255)
                                                                                                                                               cgcgGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCg    <  4:278097/147‑1 (MQ=255)
                                                                                                                                               cgcgGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCg    <  4:214914/147‑1 (MQ=255)
                                                                                                                                               cgcgGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCg    <  4:122330/147‑1 (MQ=255)
                                                                                                                                               cgcgGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCg    <  7:216792/147‑1 (MQ=255)
                                                                                                                                               cgcgGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGgt  >  3:268424/1‑149 (MQ=255)
                                                                                                                                                gcgGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACGACCTGGCTCGgt  <  7:224237/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTC‑AGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGT  >  NC_002947/4740691‑4740982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑