| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | 100% | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 100.0% | 119.3 / NA | 42 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (17/25); total (17/25) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 100.0% | 116.4 / NA | 42 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (17/25); total (17/25) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑T‑CGGG‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCT > NC_002947/1499338‑1499618 || ccGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGc < 4:206899/148‑1 (MQ=255) gACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCAATGCACCGGCttt > 3:256971/1‑149 (MQ=255) cGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGc < 2:242091/149‑1 (MQ=255) ccGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgt < 5:225708/149‑1 (MQ=255) gtCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcgc < 7:141907/148‑4 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGCAACACGCGCCGCGGGGCATTGCACCGGGGTTGCCGGTGt‑tagcggg > 1:7263/1‑143 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcgcgg < 5:10900/148‑6 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcgcgg < 4:5638/148‑6 (MQ=255) aaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCttt > 8:92171/1‑131 (MQ=255) aaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCttt < 7:92171/131‑1 (MQ=255) aaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CAc > 6:279145/1‑149 (MQ=37) cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CAcg‑c > 4:92462/1‑149 (MQ=37) cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CAcg‑c < 7:183515/149‑1 (MQ=37) aGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCGCCGAAACACGCGCCGCGGTGCCTGGGACCCGCTTTGCCGGTGT‑T‑TGCGGG‑GAcg‑cccgcgc > 8:133538/1‑144 (MQ=255) ccATTCAGCGCCTGAACGACCCCTTGGCACCACCGCAGGACTGAGGCGGCCAACCGG‑TGGGACCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTGC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTg < 8:202070/149‑1 (MQ=18) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 5:120160/148‑1 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGTCCGCGAAACAGGCGCCGCGGTGAATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTAt > 3:90520/1‑149 (MQ=25) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTAt < 5:279145/149‑1 (MQ=21) tcccTGGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 4:256971/147‑1 (MQ=21) ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGATGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TAG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa > 1:40591/1‑149 (MQ=21) ggCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTTCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTAc < 3:92462/147‑1 (MQ=21) cagGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCg > 5:92621/1‑149 (MQ=21) ggcgcacAAAGGG‑GGGGTGCGGGCGCGCCCGCTAAACTGGCGCCGGGTTGCAGGGCACCGGGTTTGGGTGTGT‑G‑C‑GGGGCCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTttgtt < 5:28353/143‑1 (MQ=255) ggCTGCCAAACGG‑TGGTAGGGGGGGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTGGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTttgt < 7:114482/148‑1 (MQ=21) gCTGCCAAACGG‑GGGGAGCGGGCGTGCCCGCTAAACCGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTttgtt < 1:107493/148‑1 (MQ=25) cTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATTGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCTCAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGc < 2:7263/149‑1 (MQ=21) tcagtctg‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTc > 5:304324/8‑149 (MQ=21) ccAAACGG‑TGGGAGCGGGCGTTCCCGCGAAACAAGCGCCGCCGTGCATGGCACCGGCGTTTCCGGTGT‑TAG‑CGGG‑CCCGCCCGCGCCCACACCCAGATTTCCATTAATCGGGCCGATAGCGACAGTGACCGGCCCttttttttttcc > 8:57257/1‑145 (MQ=255) ccAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCt < 2:40591/148‑1 (MQ=21) ggggCGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGtttttt < 7:117132/132‑1 (MQ=18) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGc > 4:58521/1‑136 (MQ=21) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGc < 3:58521/136‑1 (MQ=21) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑Cgg > 2:9103/1‑114 (MQ=18) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt > 1:27157/1‑149 (MQ=37) cGTGCCCGAGAATAAGGCGCCGCGTTGCATGGCACCGGCTTTGCGGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc < 7:133538/149‑1 (MQ=25) tGCCCGCGAAACAGGCGCCGCTGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTccc < 4:30971/149‑1 (MQ=37) gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTttgtt < 4:110306/117‑1 (MQ=21) gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTttgtt > 3:110306/1‑117 (MQ=21) aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc > 7:57598/1‑149 (MQ=37) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa < 3:108475/149‑1 (MQ=37) gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 3:133949/1‑148 (MQ=37) tGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑AACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCt < 4:67734/149‑1 (MQ=38) || CCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑T‑CGGG‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCT > NC_002947/1499338‑1499618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |