| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | 100% | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 92.1% | 86.0 / 3.5 | 38 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (13/20); total (16/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 98.7% | 94.0 / ‑6.6 | 38 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (15/21); total (16/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑T‑CGGG‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACG > NC_002947/1499358‑1499644 ||| aaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CAc > 6:279145/1‑149 (MQ=37) cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CAcg‑c > 4:92462/1‑149 (MQ=37) cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CAcg‑c < 7:183515/149‑1 (MQ=37) aGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCGCCGAAACACGCGCCGCGGTGCCTGGGACCCGCTTTGCCGGTGT‑T‑TGCGGG‑GAcg‑cccgcgc > 8:133538/1‑144 (MQ=255) ccATTCAGCGCCTGAACGACCCCTTGGCACCACCGCAGGACTGAGGCGGCCAACCGG‑TGGGACCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTGC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTg < 8:202070/149‑1 (MQ=18) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 5:120160/148‑1 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGTCCGCGAAACAGGCGCCGCGGTGAATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTAt > 3:90520/1‑149 (MQ=25) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTAt < 5:279145/149‑1 (MQ=21) tcccTGGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 4:256971/147‑1 (MQ=21) ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGATGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TAG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa > 1:40591/1‑149 (MQ=21) ggCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTTCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTAc < 3:92462/147‑1 (MQ=21) cagGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCg > 5:92621/1‑149 (MQ=21) ggcgcacAAAGGG‑GGGGTGCGGGCGCGCCCGCTAAACTGGCGCCGGGTTGCAGGGCACCGGGTTTGGGTGTGT‑G‑C‑GGGGCCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTttgtt < 5:28353/143‑1 (MQ=255) ggCTGCCAAACGG‑TGGTAGGGGGGGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTGGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTttgt < 7:114482/148‑1 (MQ=21) gCTGCCAAACGG‑GGGGAGCGGGCGTGCCCGCTAAACCGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTttgtt < 1:107493/148‑1 (MQ=25) cTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATTGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCTCAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGc < 2:7263/149‑1 (MQ=21) tcagtctg‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTc > 5:304324/8‑149 (MQ=21) ccAAACGG‑TGGGAGCGGGCGTTCCCGCGAAACAAGCGCCGCCGTGCATGGCACCGGCGTTTCCGGTGT‑TAG‑CGGG‑CCCGCCCGCGCCCACACCCAGATTTCCATTAATCGGGCCGATAGCGACAGTGACCGGCCCttttttttttcc > 8:57257/1‑145 (MQ=255) ccAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCt < 2:40591/148‑1 (MQ=21) ggggCGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGtttttt < 7:117132/132‑1 (MQ=18) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGc > 4:58521/1‑136 (MQ=21) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGc < 3:58521/136‑1 (MQ=21) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑Cgg > 2:9103/1‑114 (MQ=18) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt > 1:27157/1‑149 (MQ=37) cGTGCCCGAGAATAAGGCGCCGCGTTGCATGGCACCGGCTTTGCGGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc < 7:133538/149‑1 (MQ=25) tGCCCGCGAAACAGGCGCCGCTGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTccc < 4:30971/149‑1 (MQ=37) gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTttgtt < 4:110306/117‑1 (MQ=21) gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTttgtt > 3:110306/1‑117 (MQ=21) aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc > 7:57598/1‑149 (MQ=37) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa < 3:108475/149‑1 (MQ=37) gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 3:133949/1‑148 (MQ=37) tGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGG‑AACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCt < 4:67734/149‑1 (MQ=38) gcaccGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACg > 2:282242/5‑149 (MQ=38) cGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTcc < 4:208835/148‑1 (MQ=39) cGGCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTcc > 3:208835/1‑148 (MQ=39) gCTTTGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGa < 6:304324/149‑1 (MQ=39) cTTTGCCGGTGT‑TCG‑CGGG‑AACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑TGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAc < 6:172182/149‑1 (MQ=255) tGCCGGTGT‑TCG‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAct < 7:173398/147‑1 (MQ=255) gtgttcgcGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa > 4:37670/8‑149 (MQ=255) tgttcgcGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt < 7:57257/143‑1 (MQ=255) tcgcGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAc > 1:148210/4‑149 (MQ=255) cgcGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGGAACGTCCCGACTTCTTCCATGAcg > 3:221851/3‑149 (MQ=255) ||| AACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGG‑TGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑T‑CGGG‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACG > NC_002947/1499358‑1499644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |