Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	307,866	+C	intergenic (+881/‑988)	hslO → / → PP_0254	molecular chaperone HslO/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	307,865	1	.	C	100.0%	79.8 / NA	24	intergenic (+880/‑989)	hslO/PP_0254	molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (9/15);  total (9/15)

CAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTA  >  NC_002947/307735‑308006                                                                                                                                    |                                                                                                                                              cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTga                                                                                                                              <  6:123686/149‑1 (MQ=255)         ccTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAg                                                                                                                       <  3:37329/148‑1 (MQ=255)              tgccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGt                                                                                                                 >  8:100308/1‑149 (MQ=255)                             tACTGCCTATGCACTGCGCGGCGAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt                                                                                                  <  2:81417/149‑1 (MQ=255)                             tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt                                                                                                  <  8:134240/149‑1 (MQ=255)                             tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGt                                                                                                   <  2:28310/148‑1 (MQ=255)                                  ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTc                                                                                               <  1:127620/147‑1 (MQ=255)                                   cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTc                                                                                            <  2:7913/149‑1 (MQ=255)                                                                       aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                        <  7:131833/149‑1 (MQ=255)                                                                            aCCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTc                                                   >  7:43277/1‑149 (MQ=255)                                                                             ccTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                                  <  1:93004/149‑1 (MQ=255)                                                                             ccTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                                  >  3:119399/1‑149 (MQ=255)                                                                                     ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                          >  8:137188/1‑149 (MQ=255)                                                                                        ggCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc                                         >  1:37534/1‑147 (MQ=255)                                                                                           cTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGcc                                    >  3:107093/1‑149 (MQ=255)                                                                                             gTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCgg                                  >  7:138840/1‑149 (MQ=255)                                                                                              tCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGt                                 >  2:74588/1‑149 (MQ=255)                                                                                                          aaGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGc                     <  3:73109/149‑1 (MQ=255)                                                                                                           ataCTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACTAGCACTGTTGGGGCTAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGACAAGTGCATCTACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGcc                    <  5:70432/147‑1 (MQ=255)                                                                                                            gACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCa                   <  6:20781/149‑1 (MQ=255)                                                                                                                 cccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg              <  8:93045/149‑1 (MQ=255)                                                                                                                  ccTTTCGGCTGACGAAAGCCGTTACCTGATCGGGGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGc             <  6:124697/149‑1 (MQ=255)                                                                                                                          cTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGgcg       >  2:155253/1‑147 (MQ=255)                                                                                                                             aCGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTa  <  7:105231/149‑1 (MQ=255)                                                                                                                                    |                                                                                                                                              CAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTA  >  NC_002947/307735‑308006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑