Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,586,035	+G	intergenic (+118/+97)	PP_4061 → / ← PP_4063	hypothetical protein/long‑chain fatty acid‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,586,033	1	.	G	100.0%	45.0 / NA	14	intergenic (+116/+99)	PP_4061/PP_4063	hypothetical protein/long‑chain fatty acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (5/9);  total (5/9)

GCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCT‑CCTACCA‑GGGA‑C‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAA  >  NC_002947/4585887‑4586110                                                                                                                                                    |                                                                                  gccgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gg                                                                                 <  6:79952/149‑1 (MQ=255)   cgAGCCAGGAGGCACCTGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gg                                                                                 <  4:140898/147‑1 (MQ=255)    gAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACAACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTAGC‑ggct                                                                               >  6:26541/1‑146 (MQ=255)       ccAGGAGCCACCGGTGAAGGCTTAAGGGTAGACGACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAAc                                                                            <  5:106642/149‑1 (MQ=255)                            ttAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGGAAACCCCCA‑CCGACCA‑gggccccgg                                                     >  8:20873/1‑143 (MQ=255)                              aaGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCT‑CCTACCA‑GGGA‑ccgtgc                                                   <  8:145647/148‑6 (MQ=39)                                   tAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCT‑CCTACCA‑GGGACC‑GTGCCGGCCt                                             <  7:116031/149‑1 (MQ=39)                                     gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCT‑CCTACCA‑GGGACC‑GTGCCGGCCtt                                            <  1:179122/148‑1 (MQ=39)                                       ccACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCCCG‑ACTACCAGGGGC‑C‑GTGCCGGCCGTGaa                                         >  1:36584/1‑149 (MQ=255)                                                  gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCT‑CCTACCA‑GGGACC‑GTGCCGGCCTTGAATGCGGCGCTGc                              <  2:1762/149‑1 (MQ=39)                                                        aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTTAACCCCCTACGAACCC‑GGGC‑C‑CTGCCGGCCTTGAATTCGGGGCTGCCCCAgg                        >  6:75137/1‑149 (MQ=255)                                                                   gcgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCT‑CCTACCA‑GGGACC‑GTGCCGGCCTTGAATGCGGCGCTGcc                             >  2:37194/1‑133 (MQ=38)                                                                   gcgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCT‑CCTACCA‑GGGACC‑GTGCCGGCCTTGAATGCGGCGCTGcc                             <  1:37194/133‑1 (MQ=38)                                                                            cATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCT‑CCTACCA‑GGGA‑c                                                        <  7:35501/97‑1 (MQ=37)                                                                            cATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCT‑CCTACCA‑GGGA‑c                                                        >  8:35501/1‑97 (MQ=37)                                                                            cATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGAGCGGGCCGGCCTCTTCGCGGGTAAACCCGCT‑CCTACCA‑GGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCcgc     <  4:42734/148‑1 (MQ=38)                                                                               tGAACGGTTGAGGTTTACGGGGTGCTTGGCATGGTGATGATGTTGCATTGATCGGGCCGGCCTCTGCGCGGGTAACCCCGCT‑CCTACCA‑GGGA‑CGGTGCCGGCCTTTAATGCGGCGCTGCCCCTGGTTGGAGGGGGTTTACCCGCGaa  <  2:36584/148‑1 (MQ=255)                                                                                                                                                    |                                                                                  GCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCT‑CCTACCA‑GGGA‑C‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAA  >  NC_002947/4585887‑4586110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑