Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,580,995 C→A K134N (AAG→AAT)  ttgB ← efflux pump membrane protein TtgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,580,9950CA100.0% 93.2 / NA 28K134N (AAG→AAT) ttgBefflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/16);  total (12/16)

GCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATG  >  NC_002947/1580849‑1581130
                                                                                                                                                  |                                                                                                                                       
gCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGttatt                                                                                                                                       <  1:123030/149‑1 (MQ=255)
gCGTACTGCGCACCGAACACCTGGAAGTCACATACACCCTCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCATGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGttatt                                                                                                                                       <  5:50955/149‑1 (MQ=255)
     cTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTg                                                                                                                                  <  3:94250/149‑1 (MQ=255)
           aCCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTGGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTgg                                                                                                                            >  3:22144/1‑149 (MQ=255)
              gAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGa                                                                                                                         >  4:160498/1‑149 (MQ=255)
                    cTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGa                                                                                                                   >  7:30197/1‑149 (MQ=255)
                       gAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGAt                                                                                                                  <  1:104803/147‑1 (MQ=255)
                               cTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTtgctgc                                                                                                        >  1:162830/1‑149 (MQ=255)
                                  cacCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTtgctgct                                                                                                       <  2:35505/147‑1 (MQ=255)
                                              gcgAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGgc                                                                                         <  6:3092/149‑1 (MQ=255)
                                                       ggTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCggg                                                                                <  3:55510/149‑1 (MQ=255)
                                                           cTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTg                                                                             <  3:160498/148‑1 (MQ=255)
                                                            tGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGc                                                                           >  2:66748/1‑149 (MQ=255)
                                                               aTGTGCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAg                                                                        >  8:9137/1‑149 (MQ=255)
                                                                     gagaCGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCa                                                                   <  6:146862/148‑1 (MQ=255)
                                                                     gagaCGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAg                                                                  >  3:111065/1‑149 (MQ=255)
                                                                      agaCGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGc                                                                 <  1:46807/149‑1 (MQ=255)
                                                                        aCGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCtt                                                               <  8:30197/149‑1 (MQ=255)
                                                                                      cAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTctgtacctgta                                                 >  3:29979/1‑149 (MQ=255)
                                                                                                  ggTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGgt                                       <  1:70507/147‑1 (MQ=255)
                                                                                                      aTGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCGTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCggg                                 <  4:83592/149‑1 (MQ=255)
                                                                                                          tgcCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTg                             >  4:126476/1‑149 (MQ=255)
                                                                                                               tCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGtacttcgg                        >  4:57454/1‑142 (MQ=255)
                                                                                                                    ggAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGtt                   >  1:180916/1‑149 (MQ=255)
                                                                                                                     gagaCCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTc                  <  1:179642/149‑1 (MQ=255)
                                                                                                                         ccAGTCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGTATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAg              <  8:20599/149‑1 (MQ=255)
                                                                                                                         ccAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAg              <  8:20600/149‑1 (MQ=255)
                                                                                                                            ggCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGTATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTTCCCTGTTCGAAGGTg           <  2:146299/149‑1 (MQ=255)
                                                                                                                                     ccAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATg  <  1:66748/149‑1 (MQ=255)
                                                                                                                                     ccAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATg  >  6:13415/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                       
GCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATG  >  NC_002947/1580849‑1581130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑