Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,699,123	C→T	Q291* (CAG→TAG)	cheBC →	chemotaxis response regulator protein‑glutamate methylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,699,123	0	C	T	100.0%	92.7 / NA	28	Q291* (CAG→TAG)	cheBC	chemotaxis response regulator protein‑glutamate methylesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/13);  total (15/13)

AACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGCAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAA  >  NC_002947/1698983‑1699263                                                                                                                                             |                                                                                                                                             aaCCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCttt                                                                                                                                      >  2:23023/1‑149 (MQ=255)     tgtgAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGa                                                                                                                                  >  7:92258/1‑149 (MQ=255)       tgaatgaaATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGa                                                                                                                                  <  3:145857/147‑1 (MQ=255)                    ccGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGa                                                                                                                     <  2:30725/147‑1 (MQ=255)                         ccTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGc                                                                                                               >  3:130028/1‑148 (MQ=255)                          cTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTTGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGcag                                                                                                             <  1:122542/149‑3 (MQ=255)                          cTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCca                                                                                                             <  2:158209/149‑1 (MQ=255)                                      gtTCTTCGAGAGCGTGGCACGTTACTGGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGgtg                                                                                                  >  8:62270/1‑148 (MQ=255)                                      gtTCTTCGAGAGCGTGGCACGTTACTGGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGgtg                                                                                                  >  7:98813/1‑148 (MQ=255)                                        tcttcGAGAGCGTGGCACGTTACTGGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTAc                                                                                               >  8:146760/1‑149 (MQ=255)                                          ttcGAGAGCGTGGCACGTTACTGGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCAAGGACCAGGCCAGCAGCGCGGTGTACgg                                                                                             >  5:166299/1‑149 (MQ=255)                                              agagCGTGGCACGTTACTGGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATg                                                                                         >  5:163558/1‑149 (MQ=255)                                                   gTGGCAGGTTACTGGAATGGTGATGCAGTGGGCGTGCTGCTCTCCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCaa                                                                                    <  1:153556/149‑1 (MQ=255)                                                               tGGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCACAAGGCTGCTGCGGc                                                                        >  3:145800/1‑149 (MQ=255)                                                                          tGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATgccg                                                             >  1:152566/1‑149 (MQ=255)                                                                             aGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGccgccg                                                          >  7:148338/1‑149 (MQ=255)                                                                              gTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGt                                                         <  3:15851/149‑1 (MQ=255)                                                                                 ggCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTgg                                                       <  8:143209/148‑1 (MQ=255)                                                                                        tgctCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATccgc                                               >  4:161396/1‑149 (MQ=255)                                                                                          ctCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATccgccc                                             <  8:92258/149‑1 (MQ=255)                                                                                                      ggCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCAt                                 <  1:23023/149‑1 (MQ=255)                                                                                                                   cccAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCtt                     <  8:98813/148‑1 (MQ=255)                                                                                                                    ccAGGGCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCCTGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGACCGCATTGCCGGGCGCTTGa                   >  8:20221/1‑149 (MQ=255)                                                                                                                        ggCCTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGa               <  3:48457/149‑1 (MQ=255)                                                                                                                           cTGAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAAtt            <  8:7695/149‑1 (MQ=255)                                                                                                                             gAAGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAAtttt          >  1:44573/1‑149 (MQ=255)                                                                                                                              aaGCTGATGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGAAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAAttttt         >  6:47139/1‑149 (MQ=255)                                                                                                                                     tGCGTCAGTAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCaaaa  <  3:142583/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                             AACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGTGATGCAGTGGGCGTGCTGCTCACCGGCATGGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGCAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAA  >  NC_002947/1698983‑1699263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑