Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,733,983	G→A	A551V (GCG→GTG)	sucA ←	2‑oxoglutarate dehydrogenase subunit E1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,733,983	0	G	A	100.0%	58.4 / NA	19	A551V (GCG→GTG)	sucA	2‑oxoglutarate dehydrogenase subunit E1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/10);  total (9/10)

ATCGGCAAGCCACCGGCCTGCATCTTCTGACGGTCTTCGTAGATCTTCGACACCTGACGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGC  >  NC_002947/4733839‑4734122                                                                                                                                                 |                                                                                                                                            aTCGGCAAGCCACCGGCCTGCATCTTCTGACGGTCTTCGTAGATCTTCGACACCTGACGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCACgg                                                                                                                                          <  6:128324/148‑1 (MQ=255)  tCGGCAAGCCACCGGCCTGCATCTTCTGACGGTCTTCGTAGATCTTCGACACCTGACGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCACGGTc                                                                                                                                        <  3:135856/149‑1 (MQ=255)             ccGGCCTGCATCTTCTGACGGTCTTCGTAGATCTTCGACACCTGACGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGCTGTCGTGACGCACGGTCCAGGCATGGcc                                                                                                                             >  8:50964/1‑149 (MQ=255)                              aCGGTCTTCGTAGATCTTCGACACCTGACGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGt                                                                                                            >  2:47091/1‑149 (MQ=255)                                                   cacCTGACGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCg                                                                                       >  8:150099/1‑149 (MQ=255)                                                        gACGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTcc                                                                                  >  2:182851/1‑149 (MQ=255)                                                                      aaaCCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCtttt                                                                    <  3:81843/149‑1 (MQ=255)                                                                          cTTCCGGCAGCTCGAGGAGCTTGGCCGAGAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCTTGACGGACGGTCCTGGCATGGCCCTGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTAcc                                                                <  7:52334/149‑1 (MQ=255)                                                                          cTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTAcc                                                                >  6:167803/1‑149 (MQ=255)                                                                                     tCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGc                                                      >  3:63596/1‑148 (MQ=255)                                                                                       gAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCa                                                    <  5:167803/148‑1 (MQ=255)                                                                                        agcagcTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCAtt                                                  >  8:19035/1‑149 (MQ=255)                                                                                         gcagcTTGGCCGACAGATCCTGCAGTGTCTTGAGGTCGAAACGGGTGTCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTg                                                 <  1:124547/149‑1 (MQ=255)                                                                                                ggCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAgcg                                          >  1:157015/1‑149 (MQ=255)                                                                                                        gATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCgg                                  <  2:123698/149‑1 (MQ=255)                                                                                                             tGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACtc                             >  6:106160/1‑149 (MQ=255)                                                                                                                                      tgtCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCg    <  1:47091/149‑1 (MQ=255)                                                                                                                                        tCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGc  <  5:23345/149‑1 (MQ=255)                                                                                                                                        tCGTGACGCACGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGc  <  6:40307/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                            ATCGGCAAGCCACCGGCCTGCATCTTCTGACGGTCTTCGTAGATCTTCGACACCTGACGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGC  >  NC_002947/4733839‑4734122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑