Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,212,521	C→T	intergenic (‑55/+133)	PP_1060 ← / ← PP_1061	glutamate synthase large subunit/ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,212,521	0	C	T	100.0%	60.8 / NA	19	intergenic (‑55/+133)	PP_1060/PP_1061	glutamate synthase large subunit/ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/7);  total (12/7)

CATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACCATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTACCGTATTC  >  NC_002947/1212378‑1212664                                                                                                                                                |                                                                                                                                                cATTCGTGGTGGCGGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGc                                                                                                                                            <  8:67366/149‑1 (MQ=255)  aTTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGc                                                                                                                                            >  7:96928/1‑148 (MQ=255)              tGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGc                                                                                                                               >  4:66202/1‑149 (MQ=255)                  ggggAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTg                                                                                                                           >  3:104864/1‑149 (MQ=255)                            gCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCaaaataaaa                                                                                                                 >  6:119934/1‑149 (MQ=255)                                 ggTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCggg                                                                                                            <  8:96928/149‑1 (MQ=255)                                 ggTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCggg                                                                                                            >  6:140591/1‑149 (MQ=255)                                     aaCAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAg                                                                                                        >  5:2483/1‑149 (MQ=255)                                             acaGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAAt                                                                                                 >  1:86954/1‑148 (MQ=255)                                                 gcggcgAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATtc                                                                                            >  3:10686/1‑149 (MQ=255)                                                   ggcgAAGAAGGCATTACGACTGAGAAGGTACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGTTGTATCGAAAATCATtctc                                                                                          <  6:82288/149‑1 (MQ=255)                                                     cgAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGa                                                                                        <  1:75381/149‑1 (MQ=255)                                                         gaagGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCg                                                                                                              >  2:52926/1‑123 (MQ=255)                                                                  aCGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCgg                                                                            <  4:83779/148‑1 (MQ=255)                                                                  aCGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCgg                                                                            <  5:119934/148‑1 (MQ=255)                                                                                         tACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTAt                                                                                                                                               >  8:75901/1‑58 (MQ=255)                                                                                                                                    gCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTAc         >  3:110650/1‑149 (MQ=255)                                                                                                                                           ccGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTACCGTATTc  >  4:46929/1‑149 (MQ=255)                                                                                                                                           ccGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTACCGTATTc  <  4:10686/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                CATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACCATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTACCGTATTC  >  NC_002947/1212378‑1212664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑