Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,733,001	Δ1 bp	7.4%	coding (2634/2832 nt)	sucA ←	2‑oxoglutarate dehydrogenase subunit E1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,733,001	0	G	.	7.4%	168.2 / 7.4	54	coding (2634/2832 nt)	sucA	2‑oxoglutarate dehydrogenase subunit E1
Reads supporting (aligned to +/- strand):  ref base G (26/24);  new base . (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

CGAAGCGTAACCACAGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCGTAGTAGACCTTGC  >  NC_002947/4732854‑4733146                                                                                                                                                    |                                                                                                                                                   cGAAGCGTAACCACAGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAg                                                                                                                                                    <  5:355343/148‑1 (MQ=255)      cGTAACCACAGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCa                                                                                                                                              >  4:164513/1‑149 (MQ=255)        tAACCACAGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATg                                                                                                                                            >  5:207110/1‑149 (MQ=255)            cacaGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCATGACCCAAGACCGCATGCtt                                                                                                                                         >  6:14323/1‑148 (MQ=255)             acaGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGa                                                                                                                                       <  4:86203/149‑1 (MQ=255)              caGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACCCCAGACCGCATGCTTGAg                                                                                                                                      >  6:297688/1‑149 (MQ=255)                                gCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAg                                                                                                                   <  3:164513/149‑1 (MQ=255)                                  gTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGgt                                                                                                                               <  4:284565/135‑1 (MQ=255)                                  gTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGgt                                                                                                                               >  3:284565/1‑135 (MQ=255)                                   tCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGAt                                                                                                                >  2:18912/1‑149 (MQ=255)                                       gACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTc                                                                                                             >  4:284216/1‑148 (MQ=255)                                        aCCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACCCCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGa                                                                                                           >  2:252003/1‑149 (MQ=255)                                              gTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTAATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGAc                                                                                                          >  7:154951/1‑144 (MQ=255)                                                   ccAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTTACACCA‑ACCTCAAGCATGCGGTCTGGTGTCAggaag                                                                                                                      <  3:228866/127‑4 (MQ=25)                                                   ccAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTTACACCA‑ACCTCAAGCATGCGGTCTGGTGTCAggaag                                                                                                                      >  4:228866/1‑124 (MQ=25)                                                   ccAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTc                                                                                                 <  1:18912/148‑1 (MQ=255)                                                   ccAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTc                                                                                                 <  4:244383/148‑1 (MQ=255)                                                          cAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGAGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCAGACACCAGACCGCATGCATGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAggg                                                                                          >  6:87352/1‑148 (MQ=255)                                                            gTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAAc                                                                                       <  3:284216/149‑1 (MQ=255)                                                                   tttgtGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATAc                                                                                 <  8:154951/147‑1 (MQ=255)                                                                   gttgtGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGa                                                                                    <  1:355093/145‑1 (MQ=255)                                                                   gttgtGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGa                                                                                    >  2:355093/1‑145 (MQ=255)                                                                   gttgtGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACa                                                                                >  4:208198/1‑149 (MQ=255)                                                                        ggcggcCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAgctgc                                                                           <  8:265177/149‑1 (MQ=255)                                                                                   aTACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAg                                                                >  6:342503/1‑149 (MQ=255)                                                                                          gcATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGc                                                          >  1:227058/1‑148 (MQ=255)                                                                                          gcATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCg                                                         <  6:207110/149‑1 (MQ=255)                                                                                            aTGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGAt                                                       <  3:208198/149‑1 (MQ=255)                                                                                                 gtgCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCtt                                                  >  4:209375/1‑149 (MQ=255)                                                                                                    ctggctgCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTc                                                 >  2:128071/1‑147 (MQ=255)                                                                                                    ctggctgCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTc                                                 <  1:128071/147‑1 (MQ=255)                                                                                                     tggctgCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTATGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTcgcg                                              >  6:66655/1‑149 (MQ=255)                                                                                                            tgTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCg                                       >  8:143692/1‑149 (MQ=255)                                                                                                             gTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCg                                                                                                            <  6:4830/79‑1 (MQ=255)                                                                                                             gTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCg                                                                                                            >  5:4830/1‑79 (MQ=255)                                                                                                              tACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCgg                                      >  8:204554/1‑148 (MQ=255)                                                                                                                ccAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCAc                                   >  8:312781/1‑149 (MQ=255)                                                                                                                ccAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCAc                                   >  2:66076/1‑149 (MQ=255)                                                                                                                      gcCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGtt                             <  7:196244/149‑1 (MQ=255)                                                                                                                            gTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCTAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTc                         <  5:297688/147‑1 (MQ=255)                                                                                                                            gTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCca                       <  5:342503/149‑1 (MQ=255)                                                                                                                                aTCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTcgcg                                              <  6:114584/122‑1 (MQ=255)                                                                                                                                aTCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTcgcg                                              >  5:114584/1‑122 (MQ=255)                                                                                                                                      tctTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCgta             >  7:133043/1‑149 (MQ=255)                                                                                                                                        ttCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCTGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCgta             <  5:87352/147‑1 (MQ=255)                                                                                                                                         cccTTACACCA‑ACCTCAAGCATGCGGTCTGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGAt                                                       >  5:317791/2‑104 (MQ=25)                                                                                                                                         cccTTACACCA‑ACCTCAAGCATGCGGTCTGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGAt                                                       <  6:317791/103‑1 (MQ=25)                                                                                                                                          ccTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCgtagta          <  7:312781/148‑1 (MQ=255)                                                                                                                                           cTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCGTAGTAGa        <  3:209375/149‑1 (MQ=255)                                                                                                                                            tGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCGTAGTAGAc       >  4:239603/1‑149 (MQ=255)                                                                                                                                               cacCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGAAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCGTAGTAGACCtt    >  7:238574/1‑149 (MQ=255)                                                                                                                                                acCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCGTAGTAGACCTTg   <  3:239603/149‑1 (MQ=255)                                                                                                                                                 ccAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCGTAGTAGACCTTGc  <  8:133043/149‑1 (MQ=255)                                                                                                                                                 ccAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCGTAGTAGACCTTGc  <  2:227058/149‑1 (MQ=255)                                                                                                                                                 ccAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCGTAGTAGACCTTGc  <  1:66076/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CGAAGCGTAACCACAGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCGTAGTAGACCTTGC  >  NC_002947/4732854‑4733146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑