Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,896,415	Δ1 bp	8.0%	intergenic (+48/‑160)	PP_4304 → / → sbp‑I	VIC family cation transporter/sulfate ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,896,415	0	C	.	8.0%	150.9 / 7.1	50	intergenic (+48/‑160)	PP_4304/sbp‑I	VIC family cation transporter/sulfate ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (25/21);  new base . (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

AGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAA  >  NC_002947/4896267‑4896562                                                                                                                                                     |                                                                                                                                                       agcagcTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGgcgc                                                                                                                                                        <  4:245987/149‑1 (MQ=255)  gcagcTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGcgcg                                                                                                                                                       >  1:60713/1‑149 (MQ=255)     gcTGCAGCATGATTGCCCGACCTGCCACAAGAAGCCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCGCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGAc                                                                                                                                                    >  7:166882/1‑149 (MQ=255)        gcagcaTGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCgc                                                                                                                                                 >  5:107315/1‑148 (MQ=255)                aTTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTc                                                                                                                                         <  7:274987/149‑1 (MQ=255)                 ttGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTcc                                                                                                                                        >  8:23453/1‑149 (MQ=255)                   gCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGCGCACGGTccg                                                                                                                                       <  6:107315/148‑2 (MQ=255)                          cTGCCACAAGAAGACACACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGa                                                                                                                            <  8:106777/149‑1 (MQ=255)                             ccACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAg                                                                                                                           <  5:60809/147‑1 (MQ=255)                             ccACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGc                                                                                                                          <  1:278228/148‑1 (MQ=255)                             ccACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGc                                                                                                                          <  5:155501/148‑1 (MQ=255)                              cacaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCgg                                                                                                                        >  1:69714/1‑149 (MQ=255)                              cacaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCATCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCgg                                                                                                                        <  2:130921/149‑1 (MQ=255)                               acaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCATGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTc                                                                                                                                         >  3:234538/1‑134 (MQ=255)                                  agcagaCCCACGAGCAGGGGGGGGCGTTCTGTTCTCGCTGGGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt                                                                                                                    <  4:261988/146‑1 (MQ=255)                                  agaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt                                                                                                                    <  2:69714/149‑1 (MQ=255)                                   gaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGTGGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGATTTa                                                                                                                   <  5:323825/149‑1 (MQ=17)                                   gaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTa                                                                                                                   >  6:171315/1‑149 (MQ=255)                                    aagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTAc                                                                                                                  >  7:105112/1‑149 (MQ=255)                                      gaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTAccc                                                                                                                <  7:137442/149‑1 (MQ=255)                                         ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt                                                                                                                    >  7:291850/1‑142 (MQ=255)                                         ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt                                                                                                                    <  8:291850/142‑1 (MQ=255)                                           aCGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGTGCGGGGTTTCCcgcg                                                                                                             >  3:246457/1‑147 (MQ=255)                                                      gcggcgTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCCTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGca                                                                                                 >  4:290029/1‑148 (MQ=255)                                                                 ttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGGGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGaa                                                                                        <  8:105112/146‑1 (MQ=255)                                                                 ttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCAAATAGCCGGTAAATAAGCAACAACAGGCGCGGACCCTGGCCGGTCC‑‑‑CCCCTAGGAGCGGGTTTTACCCCGAAGACGCCCGCACAGGCACAAGc                                                                                      >  4:312300/1‑148 (MQ=255)                                                                      gCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGGACAGGCAACAGCATAAc                                                                                 >  4:213505/1‑148 (MQ=255)                                                                          tgGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGG‑TTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCaaa                                                                             <  2:60713/147‑1 (MQ=255)                                                                             gAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCaaa                                                                             >  6:272418/1‑145 (MQ=255)                                                                             gAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCaaa                                                                             <  5:272418/145‑1 (MQ=255)                                                                              aaTGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCATAGCGGGTAAATACGCACCGACAGGCGCGGCCCGGGGGCGGTCC‑‑‑CCCGTAGGCGCGGGGTTACCCGCGAAGAGGCCGGCCCACGCAGAAGCCTaaccaaaccaat                                                                        >  6:326245/1‑147 (MQ=255)                                                                                      gttTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGtt                                                                 <  3:290029/148‑1 (MQ=255)                                                                                      gttTCCAAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGTTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGGGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGtt                                                                 <  3:312300/148‑1 (MQ=255)                                                                                           ccAACGCGTGATCGCGCCTGCGCCGGCCTCTTCGCTGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTc                                                                <  7:142157/144‑1 (MQ=255)                                                                                            cAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATACGCTCCTACAGGCGCGGCCCGTGCGCGGTCC‑‑‑CCCGGAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCAGAACCCAAGCATTTTTTGTTCTGTAAc                                                          >  8:346312/1‑149 (MQ=38)                                                                                                     aTAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGGTTACCCGCGAAGAGGCCGGCCCAGGCAGAAGCAGAACCAAAGCATTTTTTGTTGTTTAACCCGTATtaa                                                 >  1:109140/1‑149 (MQ=38)                                                                                                                       tctTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGc                               >  2:165898/1‑149 (MQ=255)                                                                                                                        ctTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCCCCCGTTATAGTCGCt                              >  7:162621/1‑149 (MQ=255)                                                                                                                         ttCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTg                             >  3:88052/1‑149 (MQ=255)                                                                                                                          tCGCGGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTgg                            >  5:210295/1‑149 (MQ=17)                                                                                                                            gcgGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGATTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTgg                            <  8:330051/147‑1 (MQ=17)                                                                                                                            gcgGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGATTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTgg                            >  7:330051/1‑147 (MQ=17)                                                                                                                                   aaTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGcc                   <  7:346312/149‑1 (MQ=255)                                                                                                                                   aaTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGcc                   >  4:50773/1‑149 (MQ=255)                                                                                                                                    aTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGccc                  <  5:326245/149‑1 (MQ=255)                                                                                                                                     tCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCGGGCAATCTGccc                  >  5:109296/1‑148 (MQ=255)                                                                                                                                         cTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAaca             <  5:196614/149‑1 (MQ=255)                                                                                                                                         cTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAaca             >  6:196614/1‑149 (MQ=255)                                                                                                                                            cTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGt                                        <  6:330837/119‑1 (MQ=255)                                                                                                                                            cTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGt                                        >  5:330837/1‑119 (MQ=255)                                                                                                                                                aGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTc       >  5:124049/1‑148 (MQ=255)                                                                                                                                                aGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTc       >  8:333708/1‑148 (MQ=255)                                                                                                                                                    gcgGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACCCCCTTCAAGaa  >  5:163360/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                       AGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAA  >  NC_002947/4896267‑4896562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑