Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,417,328 Δ1 bp 13.0% coding (576/759 nt) PP_1239 → metallo‑beta‑lactamase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,417,3280T.13.0% 157.1 / 18.1 54coding (576/759 nt)PP_1239metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base T (25/22);  new base . (3/4);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGTGGCGAA  >  NC_002947/1417190‑1417474
                                                                                                                                           |                                                                                                                                                   
gCTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGCCATTTGAACAACCACCAGGCTGCGC‑GCCTg                                                                                                                                           <  1:28702/148‑1 (MQ=255)
  tGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGACGCTAACCACTGTCGCGACCTGCTGACACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTg                                                                                                                                        >  8:313579/1‑149 (MQ=255)
    gAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGC‑GCGCAGCCTGGTgg                                                                                                                                       <  6:259189/148‑2 (MQ=255)
    gAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGC‑GCGCAGCCTGGTg                                                                                                                                        <  1:56763/147‑1 (MQ=255)
             cAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTggg                                                                                                                             >  5:128380/1‑149 (MQ=255)
                ggCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTg                                                                                                                          >  6:27982/1‑149 (MQ=255)
                    tGGATGCACTGCTGATCGAGGCTACCCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGa                                                                                                                                   <  5:280017/136‑1 (MQ=255)
                    tGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCGGGTGCACGa                                                                                                                                   >  6:280017/1‑136 (MQ=255)
                      gATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGaacaa                                                                                                                    <  3:88723/149‑1 (MQ=255)
                           aCTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTg                                                                                                                >  5:84864/1‑148 (MQ=255)
                             tgctgATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCaa                                                                                                             >  1:39587/1‑149 (MQ=255)
                                 gATCGAGGCTAACCACTGTCGCGTCCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGGATGCTAGGGCATTTTAACAACCACCAGGCTGCGC‑GCCTGGTGCTCGAGTTGGGCTgg                                                                                                                         <  7:145620/133‑1 (MQ=255)
                                 gATCGAGGATAAACACTGTCGCGAACTGCTGGCACGTGGTCACTACCAGTATTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAATGGCATTTGAACAACCACCAGGCTGCGA‑GCCTGGTTCACGATTTggtcagg                                                                                                                         >  8:145620/1‑128 (MQ=255)
                                  aTCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCTTGGTGCACGAGTTGGGCTGGAACAACCTGCAacac                                                                                                          <  1:235342/147‑1 (MQ=255)
                                  aTCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTgg                                                                                                                              >  5:319109/1‑127 (MQ=255)
                                  aTCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTgg                                                                                                                              <  6:319109/127‑1 (MQ=255)
                                   tCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTg                                                                                                       <  6:154460/149‑1 (MQ=255)
                                      aGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTg                                                                                                    <  6:128380/149‑1 (MQ=255)
                                         cTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCAGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTg                                                                                                 >  5:332878/1‑149 (MQ=255)
                                          tAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAg                                                                                                                                  >  6:177591/1‑115 (MQ=255)
                                          tAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAg                                                                                                                                  <  5:177591/115‑1 (MQ=255)
                                          tAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTgg                                                                                                >  4:250962/1‑149 (MQ=255)
                                                   tCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTca                                                                                       <  8:202335/149‑1 (MQ=255)
                                                    cgcgACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTcag                                                                                      >  7:118494/1‑149 (MQ=255)
                                                      cgACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTcagca                                                                                    <  4:64190/149‑1 (MQ=255)
                                                                gCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCAcc                                                                                          <  5:12110/133‑1 (MQ=255)
                                                                gCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCAcc                                                                                          >  6:12110/1‑133 (MQ=255)
                                                                gCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGaacaa                                                                          >  1:270899/1‑149 (MQ=255)
                                                                 cACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGaacaac                                                                         <  2:39587/149‑1 (MQ=255)
                                                                     tGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGc                                                                     <  3:250962/149‑1 (MQ=255)
                                                                       gTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCca                                                                   >  2:253868/1‑149 (MQ=255)
                                                                        tCACTACCCGGCTTTTCTGAAGCAGCGGAGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCt                                                                       >  1:21047/1‑145 (MQ=255)
                                                                        tCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCt                                                                       <  2:21047/144‑1 (MQ=255)
                                                                            tACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACtt                                                              >  3:22955/1‑149 (MQ=255)
                                                                                      ttCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGcc                                                      <  8:118494/147‑1 (MQ=255)
                                                                                         tGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGc                                                 >  8:132355/1‑149 (MQ=255)
                                                                                                            gCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTg                                             >  5:157602/1‑135 (MQ=255)
                                                                                                            gCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTg                                             <  6:157602/135‑1 (MQ=255)
                                                                                                             cATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGc                                                           <  8:177683/120‑1 (MQ=255)
                                                                                                             cATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGc                                                           >  7:177683/1‑120 (MQ=255)
                                                                                                               tGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACCCCTGGTGCTGGCCCACCTCAGCAGAAAGAACAACCTGACACACTTGGCCAGCAAGTGCTTTGTCGACACCCTccgatc                             >  5:366050/1‑143 (MQ=255)
                                                                                                                  aaGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCCCCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGAc                         >  3:212735/1‑149 (MQ=255)
                                                                                                                              acaacCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCgg                                >  7:118260/1‑130 (MQ=255)
                                                                                                                              acaacCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCgg                                <  8:118260/130‑1 (MQ=255)
                                                                                                                                gtgcaccaGGC‑GCGCAGCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTc             >  2:356289/4‑147 (MQ=255)
                                                                                                                                aacCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGaacaac                                                                                                                   >  3:113847/1‑45 (MQ=255)
                                                                                                                                aacCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGaacaac                                                                                                                   <  4:113847/45‑1 (MQ=255)
                                                                                                                                 tgcaccaGGC‑GCGCAGCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCg                      <  6:274691/135‑1 (MQ=255)
                                                                                                                                 tgcaccaGGC‑GCGCAGCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCg                      >  5:274691/3‑137 (MQ=255)
                                                                                                                                 tgcaccaGGC‑GCGCAGCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAg          >  7:78430/3‑149 (MQ=255)
                                                                                                                                 accacgAGGCTGCGC‑GCCTGGTGGACGAGTTGGGCTGGAACCACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAg          <  4:22955/149‑1 (MQ=255)
                                                                                                                                    accaGGC‑GCGCAGCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGTg       <  1:356289/149‑1 (MQ=255)
                                                                                                                                     gcaGGCTGCTC‑GCGTTTTGCACGCTTTGTGCTGGAACATCCTGAAACTCTTGGTGCTTTACCACATCAGAAGCTAGTACAACATGCCACTCTTGGCCCGCCTGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGTgg      <  2:270899/148‑1 (MQ=255)
                                                                                                                                         gCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGTGGCGaa  >  2:287024/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
GCTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGG‑GTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGTGGCGAA  >  NC_002947/1417190‑1417474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑