| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,017,246 | Δ1 bp | 100% | intergenic (‑854/‑1480) | PP_1795 ← / → PP_1797 | hypothetical protein/HlyD family secretion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,017,246 | 0 | G | . | 96.4% | 113.6 / ‑1.8 | 28 | intergenic (‑854/‑1480) | PP_1795/PP_1797 | hypothetical protein/HlyD family secretion protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (15/12); minor base A (1/0); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGACATTGATCCTGACCGTCCTGTTGCTGACGCTAGGCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGTGGGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAGTCGTCATGTTCCTATTCCATCCATGG > NC_002947/2017100‑2017387 | tGACATTGATCCTGACCGTCCTGTTGCTGACGCTAGGCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTTCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGTggg > 5:42038/1‑149 (MQ=255) ttGCTGACGCTAGGCTCCCTCGAATGGGTTCGGTCGCGAATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGATCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAg < 8:224682/147‑1 (MQ=255) gACGCTAGGCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTg > 4:355423/1‑148 (MQ=255) cGCTAGGCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGGAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAAt > 6:359134/1‑149 (MQ=255) cTAGGCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACg < 1:66724/132‑1 (MQ=255) cTAGGCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACg > 2:66724/1‑132 (MQ=255) gCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCTGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGCATGACCt > 3:229733/1‑148 (MQ=255) cTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTaa > 6:69695/1‑149 (MQ=255) gAATGGGTTCGGTCGCGTATCATGGTTCCTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCtt < 5:202074/149‑1 (MQ=255) aaTGGGTTCGGTCGCGGATCATGGTCCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTa < 6:42038/149‑1 (MQ=255) tCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGttttt > 1:68881/1‑149 (MQ=255) cGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCgt < 5:69695/149‑1 (MQ=255) ataAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGtt > 2:6073/1‑149 (MQ=255) gCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGtttt < 1:358299/126‑1 (MQ=255) gCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGtttt > 2:358299/1‑126 (MQ=255) gtgCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTcc > 8:98478/1‑149 (MQ=255) aGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTcc > 1:114934/1‑149 (MQ=255) cAGGGATGGGGACCGAGCCAGCTTCAAGCGATCGCTTCACAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCa < 7:286664/149‑1 (MQ=255) tgtACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTtata < 7:98478/147‑1 (MQ=255) cTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAGTCGTCATGTTcc < 2:114934/149‑1 (MQ=255) ttCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATAc < 2:232319/134‑1 (MQ=255) ttCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATAc > 1:232319/1‑134 (MQ=255) ttCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAGTCGTCATGTTcc > 6:101423/1‑148 (MQ=255) ttCAAGCGATCGCTTCAGAG‑AGGGGGGGGCGATGATACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTACGCATTTTTCGCCGCACCGTGGTTTCCAATTTAAACAgtcgtc > 5:262894/1‑141 (MQ=255) ttCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGGTCCGTGGTTTCCCATTTATACAGTCGTCATGTTCCTATTCCATCCAtgg > 1:312875/1‑149 (MQ=255) ttCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAg > 5:59624/1‑123 (MQ=255) ttCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAg < 6:59624/123‑1 (MQ=255) ttCAGAGT‑‑GGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAGTCGTCATGTTCCTATtccatcca < 5:138098/145‑1 (MQ=255) ttCAGAGT‑‑GGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAGTCGTCATGTTCCTATtccatcca > 6:138098/1‑145 (MQ=255) | TGACATTGATCCTGACCGTCCTGTTGCTGACGCTAGGCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGTGGGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAGTCGTCATGTTCCTATTCCATCCATGG > NC_002947/2017100‑2017387 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |