Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,571,856 Δ1 bp 8.0% coding (581/1311 nt) dctA‑III ← C4‑dicarboxylate transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,571,8560A. 8.0% 154.6 / 7.3 50coding (581/1311 nt)dctA‑IIIC4‑dicarboxylate transport protein
Reads supporting (aligned to +/- strand):  ref base A (23/23);  new base . (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

ACGGTGCCCAGCACGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAAGAATGTTGTTGTCGGCGAAGGC  >  NC_002947/2571712‑2572004
                                                                                                                                                |                                                                                                                                                    
aCGGTGCCCAGCACGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGAt                                                                                                                                                  >  3:281784/1‑149 (MQ=255)
     gCCCGGCACGATCAGCACGAAGCCGCCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGc                                                                                                                                             >  7:82622/1‑149 (MQ=255)
      cccAGCACGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGc                                                                                                                                             <  3:357746/148‑1 (MQ=255)
       ccAGCACGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGCCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGcc                                                                                                                                            >  5:84178/1‑148 (MQ=255)
           cACGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCatga                                                                                                                                       <  3:198801/149‑1 (MQ=255)
            aCGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCGGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCatgat                                                                                                                                      >  3:312197/1‑149 (MQ=255)
                  aGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCACCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGaa                                                                                                                                <  6:347840/149‑1 (MQ=255)
                     aCGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGAt                                                                                                                             <  1:175957/149‑1 (MQ=255)
                       gAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATca                                                                                                                           >  6:324088/1‑149 (MQ=255)
                            cGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATgg                                                                                                                      <  2:43619/149‑1 (MQ=255)
                                     tGAGGTAGAATAGCGCCACCAGCCCGCCGAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGAGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCg                                                                                                             <  4:215884/149‑1 (MQ=255)
                                       aGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACTATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGtt                                                                                                           <  6:210252/149‑1 (MQ=255)
                                            gAACAGCGGCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATc                                                                                                       <  1:242148/148‑1 (MQ=255)
                                               cAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTc                                                                                                   >  8:82361/1‑149 (MQ=255)
                                               cAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTc                                                                                                   >  8:77401/1‑149 (MQ=255)
                                                      accaGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAAt                                                                                            >  1:95033/1‑149 (MQ=255)
                                                        caGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGc                                                                                          <  4:281784/149‑1 (MQ=255)
                                                           cccgcccAGGTGCTGCTGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCcgg                                                                                       <  4:312197/149‑1 (MQ=255)
                                                           cccgcccAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATAAGTCGCGAAATGCcgg                                                                                       >  3:233170/1‑149 (MQ=255)
                                                            ccgcccAGGTGCAGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGTGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTACGTTGATCAGTCGCGAAATGCcggc                                                                                      >  4:306245/1‑149 (MQ=255)
                                                              gcccAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCcggcgg                                                                                    <  7:119029/149‑1 (MQ=255)
                                                                 cAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCg                                                                                 <  3:323203/149‑1 (MQ=255)
                                                                  aGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGa                                                                                <  7:77401/149‑1 (MQ=255)
                                                                  aGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGa                                                                                <  7:82361/149‑1 (MQ=255)
                                                                         gcAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTcacc                                                                          >  2:290446/1‑148 (MQ=255)
                                                                            gCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTAGCGAAATGCCGGCGGCCGAGTcaacaa                                                                        >  3:224906/1‑143 (MQ=255)
                                                                             cGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACccacca                                                                     <  5:324088/149‑1 (MQ=255)
                                                                                             ttGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGcc                                                     >  2:279873/1‑149 (MQ=255)
                                                                                                   gtggtgAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAAc                                                 <  4:233170/147‑1 (MQ=255)
                                                                                                   gtggtgAAGGCGATGCCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACa                                                <  6:84178/148‑1 (MQ=255)
                                                                                                         aaGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCATGGCGCTGCCGAACAGCAcc                                           >  8:288761/1‑147 (MQ=255)
                                                                                                               aTGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAAcag                                   <  1:290446/149‑1 (MQ=255)
                                                                                                               aTGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAAcag                                   >  1:308362/1‑149 (MQ=255)
                                                                                                                    aCCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAAc                                                 <  6:54944/130‑1 (MQ=255)
                                                                                                                    aCCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAAc                                                 >  5:54944/1‑130 (MQ=255)
                                                                                                                    aCCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAgcagca                              >  4:282595/1‑149 (MQ=255)
                                                                                                                          cacGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTg                          >  8:10411/1‑147 (MQ=255)
                                                                                                                             gCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCTAACAGCACCGAGAACAGCAGCACCTgaagaa                     >  6:51145/1‑149 (MQ=255)
                                                                                                                             gCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAAca                                    >  6:305330/1‑134 (MQ=255)
                                                                                                                             gCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAAca                                    <  5:305330/134‑1 (MQ=255)
                                                                                                                               gatcgtgcgcCTGGCGC‑CGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAAGAAtgt                  >  3:262703/7‑149 (MQ=255)
                                                                                                                               gatcgtgcgcCTGGCGC‑CGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAAGAAtg                   <  4:262703/142‑1 (MQ=255)
                                                                                                                                atcgtgcgcCTGGCGC‑CGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTgaa                        <  4:320648/137‑1 (MQ=255)
                                                                                                                                atcgtgcgcCTGGCGC‑CGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTgaa                        >  3:320648/6‑142 (MQ=255)
                                                                                                                                    ggCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAATAAtgttgtt              <  3:282595/149‑1 (MQ=255)
                                                                                                                                     gcgcCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAAGAAtgttgtt              <  3:104992/148‑1 (MQ=255)
                                                                                                                                     gcgcCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAAGAAtgttgtt              >  4:104992/1‑148 (MQ=255)
                                                                                                                                         cAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGGACCGAGAACAGCAGCACCTGAAGAATGTTGTTGTCgg         >  2:76396/1‑149 (MQ=255)
                                                                                                                                              gcACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAAt                                                                                            <  6:288053/61‑1 (MQ=255)
                                                                                                                                              gcACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAAt                                                                                            >  5:288053/1‑61 (MQ=255)
                                                                                                                                                aCGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAAGAATGTTGTTGTCGGCGAAGGc  <  1:279873/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
ACGGTGCCCAGCACGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAAGAATGTTGTTGTCGGCGAAGGC  >  NC_002947/2571712‑2572004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑