Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,682,914 Δ1 bp 8.9% coding (1480/1890 nt) prpE → propionyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,682,9140A. 8.9% 164.2 / 10.8 56coding (1480/1890 nt)prpEpropionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (26/25);  new base . (2/3);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

CTGCCATTGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAGGTACCGTTGGCGCTGGTG  >  NC_002947/2682767‑2683057
                                                                                                                                                    |                                                                                                                                               
cTGCCATTGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCAc                                                                                                                                                >  3:28462/1‑149 (MQ=255)
      ttGCCCCCCGGTTGCAGTCAGACGCTGTGGGGGGATCCCGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTGCGTCTACATCAT‑GGGGCGCACCgatga                                                                                                                                          >  5:183211/1‑149 (MQ=255)
      ttGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCgatga                                                                                                                                          <  4:101980/149‑1 (MQ=255)
      ttGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCgatga                                                                                                                                          >  3:310994/1‑149 (MQ=255)
      ttGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCgatga                                                                                                                                          >  4:96041/1‑149 (MQ=255)
          cACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACATGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCTCACCGATGAtgt                                                                                                                                       >  4:349616/1‑148 (MQ=255)
               ggTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCATCGGTGCGC‑CCCATGAtgtagacga                                                                                                                                 <  7:68675/149‑7 (MQ=255)
               ggTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCaa                                                                                                                                 <  4:28462/149‑1 (MQ=255)
                  tGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGt                                                                                                                              >  4:130062/1‑149 (MQ=255)
                     aGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCATCGGTGCGC‑CCCATGAtgt                                                                                                                                       >  2:32059/1‑137 (MQ=255)
                     aGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTCCGTCTACATCATCGGTGCGC‑CCCATGAtgt                                                                                                                                       <  1:32059/137‑1 (MQ=255)
                      gTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGTCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTcc                                                                                                                          <  2:316969/149‑1 (MQ=255)
                               tgtgGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGt                                                                                                                 >  5:254721/1‑149 (MQ=255)
                                 tgGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGt                                                                                                                 <  4:310994/147‑1 (MQ=255)
                                   gggggATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGt                                                                                                             >  8:15313/1‑149 (MQ=255)
                                      ggATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCa                                                                                                          >  1:163086/1‑149 (MQ=255)
                                                  cTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCATCGGTGCGC‑CCCATGAtgta                                                                                                                                      >  4:186357/1‑108 (MQ=255)
                                                  cTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCATCGGTGCGC‑CCCATGAtgta                                                                                                                                      <  3:186357/109‑2 (MQ=255)
                                                   tACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATgg                                                                                              >  4:331732/1‑148 (MQ=255)
                                                      cTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGa                                                                                          >  5:39464/1‑149 (MQ=255)
                                                        gCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGAcc                                                                                        >  1:54548/1‑149 (MQ=255)
                                                        gCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGAcc                                                                                        >  3:47784/1‑149 (MQ=255)
                                                          aGGCCTACCTGCTCACGTATCCTGTGTACTACCAGACGGGCTATGGCGGCTATCTGTCAGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCt                                                                                       <  3:349616/148‑1 (MQ=255)
                                                          aGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTg                                                                                      <  3:96041/149‑1 (MQ=255)
                                                               tACCTGCGCACGTATCCGGGGTACTACCCCACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTcgc                                                                                 >  4:331023/1‑149 (MQ=255)
                                                                      gcACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCAc                                                                          >  4:58605/1‑149 (MQ=255)
                                                                        aCGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCAccc                                                                        >  8:6407/1‑149 (MQ=255)
                                                                        aCGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCAccc                                                                        >  1:66111/1‑149 (MQ=255)
                                                                               cGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATgg                                                                                              <  1:318409/120‑1 (MQ=255)
                                                                               cGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATgg                                                                                              >  2:318409/1‑120 (MQ=255)
                                                                                 gggTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGc                                                               <  7:15313/149‑1 (MQ=255)
                                                                                 gggTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGc                                                               <  3:130062/149‑1 (MQ=255)
                                                                                  ggTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCg                                                              <  5:189146/149‑1 (MQ=255)
                                                                                      cttccACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAgt                                                          <  6:254721/146‑1 (MQ=255)
                                                                                        acCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAgtgt                                                        >  4:120684/1‑149 (MQ=255)
                                                                                         ccACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGa                                                            <  4:277153/144‑1 (MQ=255)
                                                                                         ccACACGGGCGATGGCGGCTAGCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGa                                                            >  3:277153/1‑144 (MQ=255)
                                                                                           acacGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAgt                                                          >  1:286008/1‑144 (MQ=255)
                                                                                           acacGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAgt                                                          <  2:286008/144‑1 (MQ=255)
                                                                                            cacGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGGCACCCGGCGGTAGCGGAGTTTGCtg                                                    <  2:66111/149‑1 (MQ=255)
                                                                                            cacGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCtg                                                    <  2:54548/149‑1 (MQ=255)
                                                                                             acGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCcggcg                                                                    >  2:257341/1‑132 (MQ=255)
                                                                                             acGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGATTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCcggcg                                                                    <  1:257341/132‑1 (MQ=255)
                                                                                                    aTGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGgt                                            <  7:6407/149‑1 (MQ=255)
                                                                                                      ggcggcTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGgtg                                           <  6:39464/148‑1 (MQ=255)
                                                                                                          gcTATCTTGACTATGATGTTTTCGTCTACATCAT‑GGTGCGGACCGATTATGTGATCTACGTTTCCCGTCATCGTTTGTCCACCGGTTAAATGGAAGACCTGGTCGCCCGCCAACCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCAt                                      <  7:95529/149‑1 (MQ=255)
                                                                                                                     gatgatGGTTTCGTCTACATCAT‑GGGGCGGACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCaa                           <  3:120684/149‑1 (MQ=255)
                                                                                                                     gatgatGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCaa                           <  3:331023/149‑1 (MQ=255)
                                                                                                                        gatgGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCGACCGGTGATATGGAAGAGCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAggg                        >  2:223101/1‑149 (MQ=255)
                                                                                                                         atgGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGc                       >  4:489/1‑149 (MQ=255)
                                                                                                                            gTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAg                    <  3:58605/149‑1 (MQ=255)
                                                                                                                                       atcat‑GGGGCGCACCGATGATGTGATCAACGTTCCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGgtg                                           <  2:82873/115‑1 (MQ=255)
                                                                                                                                       atcat‑GGGGCGCACCGATGATGTGATCAACGTTACCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGgtg                                           >  1:82873/1‑115 (MQ=255)
                                                                                                                                             ggggCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCCGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCACGTACCGTTGGCGCtgg    >  6:297948/1‑149 (MQ=255)
                                                                                                                                              gggCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAGGTACCGTTGGCGCtggt   <  3:489/149‑1 (MQ=255)
                                                                                                                                               ggCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAGGTACCGTTGGCGCtggcg  <  3:331732/149‑3 (MQ=255)
                                                                                                                                                    aCCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACgaga                               >  5:89659/1‑115 (MQ=255)
                                                                                                                                                    aCCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACgaga                               <  6:89659/115‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CTGCCATTGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAGGTACCGTTGGCGCTGGTG  >  NC_002947/2682767‑2683057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑