Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,100,569	Δ1 bp	8.5%	coding (1348/1470 nt)	PP_2709 →	long‑chain fatty acid‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,100,569	0	G	.	8.5%	177.0 / 11.2	59	coding (1348/1470 nt)	PP_2709	long‑chain fatty acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (27/25);  new base . (2/3);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

GTGATCGCCCAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCTTTTTCGACACC  >  NC_002947/3100422‑3100717                                                                                                                                                      |                                                                                                                                                     gtgAGCGCCCCTGGTTTCGGTGATGGCGTAGCCTTGCCCCGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGc                                                                                                                                                     <  8:52766/149‑1 (MQ=255)        cccAGGCTTTCTTGCATGGCGAAGCCTTGCCACGCAACCATGCCTTGATCTGGCCGCTGGATCCCGGCATCAGCGACGAGCCCATCGAACAGGCCGTACAGCAATGCAACGCGCGAATGCCCGATTACGCCAGG‑GTGCA‑CGCCTGgcgc                                                                                                                                              >  1:137775/1‑149 (MQ=255)        cccAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGgcgc                                                                                                                                              >  8:86175/1‑149 (MQ=255)        cccAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGgcgc                                                                                                                                              >  7:75185/1‑149 (MQ=255)           aGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGcgccgc                                                                                                                                           >  8:183880/1‑149 (MQ=255)             gCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGGCGTGCA‑C‑CCTGgcgtaatcg                                                                                                                                         <  8:322479/149‑7 (MQ=255)                    tGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGGCGTGCA‑C‑CCTGgcg                                                                                                                                               <  2:138728/136‑1 (MQ=255)                    tGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGGCGTGCA‑C‑CCTGgcg                                                                                                                                               >  1:138728/1‑136 (MQ=255)                      cATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGc                                                                                                                                 <  3:180943/148‑1 (MQ=255)                           cGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCctct                                                                                                                             >  1:93996/1‑147 (MQ=255)                           cGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTa                                                                                                                           <  3:185745/149‑1 (MQ=255)                                 cTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGc                                                                                                                     <  8:75185/149‑1 (MQ=255)                                  ttGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCt                                                                                                                    >  5:243213/1‑149 (MQ=255)                                    gCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGTCAGTGCCTCTTAGCACGCtt                                                                                                                   <  4:192021/148‑1 (MQ=255)                                       aCGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGc                                                                                                                                 >  6:106835/1‑131 (MQ=255)                                       aCGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGc                                                                                                                                 <  5:106835/131‑1 (MQ=255)                                           aaCCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAAc                                                                                                           >  7:217527/1‑149 (MQ=255)                                             ccttgccttgCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAAccc                                                                                                         >  3:112138/1‑149 (MQ=255)                                               ttgccttgcTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGaaa                                                                                                            >  4:303808/1‑144 (MQ=255)                                               ttgccttgcTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGaaa                                                                                                            <  3:303808/144‑1 (MQ=255)                                                  ccttgcTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGAc                                                                                                     <  4:20909/148‑1 (MQ=255)                                                     tgcTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CTCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGc                                                                                                  >  3:319683/1‑148 (MQ=255)                                                      gcTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGc                                                                                                  <  7:183880/147‑1 (MQ=255)                                                       ctctGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCaa                                                                                               >  7:136965/1‑149 (MQ=255)                                                            gCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGgc                                                                                           <  3:135091/148‑1 (MQ=255)                                                              cGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGgccgc                                                                                        >  7:159302/1‑149 (MQ=255)                                                               gCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGgccgcc                                                                                       >  8:216451/1‑149 (MQ=255)                                                                       ccGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGc                                                                                                  <  6:265016/130‑1 (MQ=255)                                                                       ccGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGc                                                                                                  >  5:265016/1‑130 (MQ=255)                                                                       ccGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGt                                                                               >  2:224949/1‑149 (MQ=255)                                                                        cGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGt                                                                               >  7:272504/1‑148 (MQ=255)                                                                          gCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAg                                                                            <  7:216451/149‑1 (MQ=255)                                                                              cAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGa                                                                        >  5:3738/1‑149 (MQ=255)                                                                                       ggCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGc                                                               <  6:243213/149‑1 (MQ=255)                                                                                        gCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CTCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCg                                                              <  4:319683/149‑1 (MQ=255)                                                                                         ccATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGt                                                             <  1:224949/149‑1 (MQ=255)                                                                                          cATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGt                                                             >  8:105133/1‑148 (MQ=255)                                                                                          cATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGt                                                             >  7:268519/1‑148 (MQ=255)                                                                                          cATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATAGCCGCCCGCGACGTGAGGCGATCTTGCAGCGt                                                             >  2:31291/1‑148 (MQ=255)                                                                                           aTCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTa                                                           >  3:140634/1‑149 (MQ=255)                                                                                           aTCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTa                                                           >  7:85901/1‑149 (MQ=255)                                                                                                aCAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACcaca                                                      <  4:112138/149‑1 (MQ=255)                                                                                                 cAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCacac                                                     >  6:280606/1‑149 (MQ=255)                                                                                                     ccGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCtg                                                 <  8:268519/149‑1 (MQ=255)                                                                                                             cAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGa                                         <  6:3738/149‑1 (MQ=255)                                                                                                               aTGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACa                                       <  8:217527/149‑1 (MQ=255)                                                                                                               aTGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACa                                       <  4:140634/149‑1 (MQ=255)                                                                                                                 gCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCAGCGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGGGGCGATCTTg                                                                   >  1:232086/1‑120 (MQ=255)                                                                                                                 gCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGGGGCGATCTTg                                                                   <  2:232086/119‑1 (MQ=255)                                                                                                                      gcgcgATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGtt                                <  5:280606/149‑1 (MQ=255)                                                                                                                      gcgcgATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTCCCTCTTAGCTCGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGtt                                <  2:93996/149‑1 (MQ=255)                                                                                                                           aTTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAg                           <  7:86175/149‑1 (MQ=255)                                                                                                                                        gCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTcc              >  7:34460/1‑149 (MQ=255)                                                                                                                                         ccAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTc               >  2:117591/1‑147 (MQ=255)                                                                                                                                         ccAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTc               <  1:117591/147‑1 (MQ=255)                                                                                                                                          cAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCtt            >  3:179260/1‑149 (MQ=255)                                                                                                                                               gTGCA‑TGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCTTTTTc        <  5:46278/149‑1 (MQ=255)                                                                                                                                               gTGCA‑C‑CCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGa                            <  3:229239/128‑1 (MQ=255)                                                                                                                                               gTGCA‑C‑CCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGa                            >  4:229239/1‑128 (MQ=255)                                                                                                                                                      gCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCTTTTTCGACAcc  <  8:136965/149‑1 (MQ=255)                                                                                                                                                      gCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCTTTTTCGACAcc  <  8:34460/149‑1 (MQ=255)                                                                                                                                                      gCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCTTTTTCGACAcc  <  4:179260/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     GTGATCGCCCAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCTTTTTCGACACC  >  NC_002947/3100422‑3100717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑