| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,054,193:1 | +T | 8.9% | coding (767/1320 nt) | PP_3573 → | monooxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,054,193 | 1 | . | T | 8.9% | 209.1 / 14.8 | 56 | coding (767/1320 nt) | PP_3573 | monooxygenase |
| Reads supporting (aligned to +/- strand): ref base . (21/30); new base T (3/2); total (24/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CGCAACCGCGACTTCTGCTGGTGGCTGGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAACGCAACGGCCTGG > NC_002947/4054048‑4054339 | cGCAACCGCGACTTCTGCTGGTGGCTGGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CAt < 1:167568/149‑1 (MQ=255) cgACTTCTGCTGGTGGCTGGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTgg < 2:88795/149‑1 (MQ=255) gACTTCTGCTGGTGGCTGGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGt < 6:34841/149‑1 (MQ=255) ctgctgGTGGCTGGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGc < 1:255613/148‑1 (MQ=255) tgctgGTGGCTGGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGAGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCt < 6:34068/149‑1 (MQ=255) ctgGTGGCTGGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGa < 8:245648/149‑1 (MQ=255) ctgGTGGCTGGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGa < 6:110933/149‑1 (MQ=255) gtggCTGGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCGACCAGGGTCATG‑CCCTGGTGGGcgaggg < 7:204177/147‑6 (MQ=255) cTGGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTc < 3:128092/149‑1 (MQ=255) tGGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCTACCAGGGTCATG‑CCCTGGTggg < 1:277775/136‑1 (MQ=255) tGGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCTACCAGGGTCATG‑CCCTGGTggg > 2:277775/1‑136 (MQ=255) gCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGAcc < 5:292271/140‑1 (MQ=255) gCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGAcc > 6:292271/1‑140 (MQ=255) gCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGaa > 6:193769/1‑148 (MQ=255) ggACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTcgcgc > 5:180472/1‑149 (MQ=255) aCGCGGAAATTGCCAAGCCCGGTCGCGAGCATGGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCAGCCTGACCCAGTCGTTCGAAAACGGc > 1:43068/1‑141 (MQ=255) aCGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCgcgcgc < 4:303342/149‑1 (MQ=255) aCGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCgcgcgc < 3:349826/149‑1 (MQ=255) aCGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCAGCCTGACCCAGTCGTTCGAAAACGGc < 2:43068/140‑1 (MQ=255) gcgGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCtt > 6:316468/1‑149 (MQ=255) gcgGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCtt > 1:139590/1‑149 (MQ=255) cgGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTc < 6:41521/149‑1 (MQ=255) aaTTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGTCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAgg < 5:193769/149‑1 (MQ=255) aaTTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGAcc > 6:191384/1‑115 (MQ=255) aaTTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGAcc < 5:191384/115‑1 (MQ=255) ccAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAAc < 5:256665/149‑1 (MQ=255) cAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAAcc > 7:43861/1‑149 (MQ=255) cAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAAcc > 8:18204/1‑149 (MQ=255) cccGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGt < 1:229599/149‑1 (MQ=255) ccGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTc < 2:139590/149‑1 (MQ=255) tCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCgaga < 1:206022/149‑1 (MQ=255) gAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACAt > 3:89326/1‑149 (MQ=255) gtgACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATcg > 1:153241/1‑149 (MQ=255) tgACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATcgc > 4:26284/1‑149 (MQ=255) aCCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCgcgg > 5:180630/1‑149 (MQ=255) cATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCgcggc > 5:359233/1‑148 (MQ=255) aTCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTGCCGTGCCCTCGCCCACCAGGG‑CATGACCCTCGACGGCCTGACCCAGTCGTACGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGcga > 8:253783/1‑149 (MQ=255) gtggCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCctgct < 1:75806/148‑1 (MQ=255) tggCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTgg < 6:180630/149‑1 (MQ=255) gCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGAt < 5:222476/149‑1 (MQ=255) cggggACTTCCGTGCCCGCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACTGCGTCGCGCGCTTCCATGACAACCTGGTCGAGAACATCAATCgcgg < 4:37741/111‑1 (MQ=255) cGTGGACTTACGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGCCCCAGTCGTGCGAAAACGGAGTCGCGCGCTTCCAGGACAACCGGGTCGAGAACATCAATCgcgg > 3:37741/1‑114 (MQ=255) gTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACg < 4:235884/67‑1 (MQ=255) gTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACg > 3:235884/1‑67 (MQ=255) tGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGAt < 3:26284/149‑1 (MQ=255) aCTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGcc < 7:18204/149‑1 (MQ=255) cTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCt < 6:359233/149‑1 (MQ=255) gTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTgg < 3:28968/120‑1 (MQ=255) gTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGCTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATc < 3:230528/149‑1 (MQ=255) gTGCCCTCGCCCACCAGGG‑CATGACACTGGTCGGCCTGACCCAGTCGATCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTgg > 4:28968/1‑120 (MQ=255) ccTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAAc > 3:241145/1‑149 (MQ=255) ccTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAAc > 7:284044/1‑149 (MQ=255) cgaccaGGGTCATG‑CCCTGGTGGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAACGCAACg > 2:128337/3‑149 (MQ=255) cgaccaGGGTCATG‑CCCTGGTGGGCCTGACCCAGTCGTTCGAAAACGGCGACGCGCGCATCCAGGACAACCTGGTCGAGAACAACAATCGAGGCGACGACCACTACCTGGACCTGCAGCATGCGGCCGATGCCTACATCGAACGCaa > 4:112288/3‑147 (MQ=255) ccaccaGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGcgacga > 6:156754/1‑99 (MQ=255) ccaccaGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGcgacga < 5:156754/99‑1 (MQ=255) ggg‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAACGCAACGGCCTgg < 4:241145/149‑1 (MQ=255) | CGCAACCGCGACTTCTGCTGGTGGCTGGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCAT‑GTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAACGCAACGGCCTGG > NC_002947/4054048‑4054339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |