Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 336,124:1 +T 100% coding (209/237 nt) PP_0278 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947336,1241.T100.0% 165.6 / ‑6.8 52D70V (GAT→GTT) PP_0278hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base T (27/23);  minor base G (0/1);  total (28/24)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGAT  >  NC_002947/335977‑336266
                                                                                                                                                    |                                                                                                                                                 
ttAACCCATCCCTCAATATCCTACGAGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATACTGATGGGATACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGTGGGCAGCCGA‑t                                                                                                                                                  >  5:80133/1‑149 (MQ=255)
     ccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttacct                                                                                                                                             >  8:254786/1‑144 (MQ=255)
        tCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCACGTCGACGCGGACTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGACGATTACC‑tgtg                                                                                                                                          >  5:118130/1‑149 (MQ=255)
              aaTCTCCTACGAGTAGAGCGCGGCGTCCCCGTCGACGCGGCCTACGATCATGTCTCGATCCTGTTGGGGTACATCAAGCACCTGGTGCGGGATGGCGATATGGAGGACGTCCATAAATTCCTGGGGGCAGGCGATTACC‑TGTGCGAc                                                                                                                                      <  6:80133/147‑1 (MQ=255)
               aTCTCCTCCGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCGCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATg                                                                                                                                   <  4:44665/149‑1 (MQ=255)
                tctcCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATgg                                                                                                                                  <  7:254786/149‑1 (MQ=255)
                       cgcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGgc                                                                                                                           <  6:218337/149‑1 (MQ=255)
                       cgcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TTCCGTTTCCGACATGGCAAAgcc                                                                                                                           >  8:19697/1‑147 (MQ=255)
                                cccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGAc                                                                                                                                      <  8:333327/129‑1 (MQ=255)
                                cccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGAGATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGAc                                                                                                                                      >  7:333327/1‑129 (MQ=255)
                                aacGGCGTCCCAGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCTCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTTCGACATGGCCAAGGCGCttatgc                                                                                                                   >  1:75598/3‑147 (MQ=255)
                                 cctgCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGCTGCGCGAGGGTGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAAc                                                                                                                 <  7:160230/146‑1 (MQ=255)
                                      gTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGatat                                                                                                            <  2:216958/149‑1 (MQ=255)
                                         cccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                           >  7:66843/1‑147 (MQ=255)
                                                aCGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGc                                                                                                 >  3:243234/1‑148 (MQ=255)
                                                       cTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGAGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGAGACATGGCCAACGCGCTGATGAACGATATCGA‑‑GATCGCAAAGGGt                                                                                          >  4:233080/1‑148 (MQ=255)
                                                        tACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGt                                                                                          >  5:59518/1‑147 (MQ=255)
                                                           gAGCATGTCTCGATGCTGATGTGCTACATCAAGCACCTGGTGCTCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑TATCGCCAAGGGTAAGgc                                                                                     <  8:66843/149‑1 (MQ=255)
                                                             gCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGgcg                                                                                    >  1:81095/1‑148 (MQ=255)
                                                               aTGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCa                                                                                  <  6:118130/148‑1 (MQ=255)
                                                                 gTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCAt                                                                                 >  1:162496/1‑147 (MQ=255)
                                                                  tctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAg                                                                              >  4:126710/1‑149 (MQ=255)
                                                                   ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGAGGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAgg                                                                             >  3:305359/1‑149 (MQ=255)
                                                                   ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAgg                                                                             >  3:310415/1‑149 (MQ=255)
                                                                   ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAg                                                                              >  7:147848/1‑148 (MQ=255)
                                                                   ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAg                                                                              <  8:147848/148‑1 (MQ=255)
                                                                         ccTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTACTGGGGGCAGACGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGa                                                                       >  5:15685/1‑149 (MQ=255)
                                                                                 gCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCa                                                               >  3:319639/1‑149 (MQ=255)
                                                                                     cATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtg                                                           >  8:272454/1‑149 (MQ=255)
                                                                                       tCAAGCACCTGGTGCGCGAGGGCGATATTGAGGAGCACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                         <  4:305359/149‑1 (MQ=255)
                                                                                       tCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                         <  4:310415/149‑1 (MQ=255)
                                                                                         aaGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                        <  3:233080/148‑1 (MQ=255)
                                                                                         aaGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                        <  1:49042/148‑1 (MQ=255)
                                                                                          aGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATTGAAGGATCGCAAAGGGTAAGGCGCATTAGGAATAGACGCGGTATTTTGTgg                                                        >  1:297725/1‑149 (MQ=255)
                                                                                          aGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGa                                                      <  7:272454/149‑1 (MQ=255)
                                                                                           gCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGAGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAgg                                                                                            >  8:103603/1‑110 (MQ=255)
                                                                                           gCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGAGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAgg                                                                                            <  7:103603/110‑1 (MQ=255)
                                                                                            cACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCaa                                                                                              >  8:192914/1‑107 (MQ=255)
                                                                                               cTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                         <  8:63987/141‑1 (MQ=255)
                                                                                               cTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                         >  7:63987/1‑141 (MQ=255)
                                                                                                     cgcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGc                                            <  3:126710/148‑1 (MQ=255)
                                                                                                              gATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                          >  2:253427/1‑125 (MQ=255)
                                                                                                              gATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                          <  1:253427/125‑1 (MQ=255)
                                                                                                                tatGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGgcgc                                                                                   <  5:232883/98‑1 (MQ=255)
                                                                                                                tatGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGgcgc                                                                                   >  6:232883/1‑98 (MQ=255)
                                                                                                                   ggaggaCGACCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTg                             >  5:92104/1‑149 (MQ=255)
                                                                                                                          gacCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAag                        >  7:119494/1‑147 (MQ=255)
                                                                                                                          gacCATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAag                        <  1:143289/147‑1 (MQ=255)
                                                                                                                             cATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGc                                            >  8:16619/1‑124 (MQ=255)
                                                                                                                             cATAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGc                                            <  7:16619/124‑1 (MQ=255)
                                                                                                                               tAAATTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGcccc                 >  1:140990/1‑149 (MQ=255)
                                                                                                                                  aTTCCTGGGGGCAGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCTTTGCAGcccattc              <  6:59518/149‑5 (MQ=39)
                                                                                                                                         ggggCAGCCGAGTACC‑TGTGCCACATGGCCAAGGCGCTGATGAACGCTATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGTTCATTTGTGTGATCGCCCTGGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGAttt         <  6:15685/147‑1 (MQ=255)
                                                                                                                                              aGCCGATTACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCATGCGTAGCCAATTGGCTGGCAAGCCCCCCCCGGCAGTTGAGAGAt  >  5:122928/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACC‑TGTGCGACATGGCCAAGGCGCTGATGAACGATATCGA‑‑GATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGAT  >  NC_002947/335977‑336266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑