Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,826,277:1	+G	6.1%	coding (4913/12954 nt)	pvdL ←	non‑ribosomal peptide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,826,277	1	.	G	6.1%	265.2 / 10.1	66	coding (4913/12954 nt)	pvdL	non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base . (31/31);  new base G (2/2);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

GCTGGCTTCGAACAGGGCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCA  >  NC_002947/4826132‑4826424                                                                                                                                                   |                                                                                                                                                        gCTGGCTTCGAACAGGGCCTTGAGCGGCAGATCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCgc‑gc                                                                                                                                                       >  3:133885/1‑148 (MQ=255)  cTGGCTTCGAACAGGGCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCgc‑gc                                                                                                                                                       >  5:206403/1‑147 (MQ=255)   tGGCTTCGAACAGGGCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCgc‑gc                                                                                                                                                       >  1:320844/1‑146 (MQ=255)   tGGCTTCGAACAGGGCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCgc‑gc                                                                                                                                                       >  2:288215/1‑146 (MQ=255)     gCTTCGAACAGGGCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGa                                                                                                                                                   <  2:320844/148‑1 (MQ=255)          gAACAGGGCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCcg                                                                                                                                             >  5:282960/1‑149 (MQ=255)           aaCAGGGCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGAt                                                                                                                                                  <  8:269088/143‑1 (MQ=255)           aaCAGGGCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGAt                                                                                                                                                  >  7:269088/1‑143 (MQ=255)                ggCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCt                                                                                                                                       >  6:235375/1‑149 (MQ=255)                 gCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCtg                                                                                                                                      <  1:288215/149‑1 (MQ=255)                       aGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGCCCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGttgttg                                                                                                                                <  8:219496/149‑1 (MQ=255)                           gCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGc                                                                                                                            >  7:105504/1‑149 (MQ=255)                              gCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCg                                                                                                                         <  5:235375/149‑1 (MQ=255)                               cTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCgg                                                                                                                        >  4:174042/1‑149 (MQ=255)                               cTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCgg                                                                                                                        >  3:151666/1‑149 (MQ=255)                                  gACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGTCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCTGTTc                                                                                                                     >  6:20492/1‑149 (MQ=255)                                  gACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGtt                                                                                                                      <  3:151827/148‑1 (MQ=255)                                   aCATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCg                                                                                                                    >  1:78788/1‑149 (MQ=255)                                     aTCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTg                                                                                                                  <  4:346200/149‑1 (MQ=255)                                      tCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTg‑‑‑g                                                                                                              <  8:316659/149‑1 (MQ=255)                                      tCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTg‑‑‑g                                                                                                              <  3:174042/149‑1 (MQ=255)                                      tCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATACAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTg‑‑‑g                                                                                                              >  2:199038/1‑149 (MQ=255)                                        gCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCt                                                                                                            <  8:195632/149‑1 (MQ=255)                                          aGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCg                                                                                                          <  2:78788/149‑1 (MQ=255)                                                ggCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGc                                                                                                    >  1:240094/1‑149 (MQ=255)                                                    ggTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCAc                                                                                                >  6:185332/1‑149 (MQ=255)                                                      tGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACgc                                                                                              >  7:116212/1‑149 (MQ=255)                                                          cgAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACgctg‑ct                                                                                         <  5:185332/149‑1 (MQ=255)                                                          cgAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACgctg‑ct                                                                                         <  5:204516/149‑1 (MQ=255)                                                           gAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGctg‑ctg                                                                                        >  7:133259/1‑149 (MQ=255)                                                                gATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GATCGACATGCTCACGCTG‑CTGcccaa                                                                                   >  7:196171/1‑146 (MQ=255)                                                                    tGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTgg                                                                               <  4:133885/149‑1 (MQ=255)                                                                      ggTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑Gc                                                                                                             <  3:324515/118‑1 (MQ=255)                                                                      ggTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑Gc                                                                                                             >  4:324515/1‑118 (MQ=255)                                                                      ggTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCacac                                                                                  <  8:116212/144‑1 (MQ=255)                                                                            cAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTGCCTGCCCCAGTGTggcagca                                                                        <  8:105504/149‑3 (MQ=255)                                                                            cAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGggcaggca                                                                       <  6:282960/149‑1 (MQ=255)                                                                               cAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCcgc                                                                                                                                            >  6:294958/1‑81 (MQ=255)                                                                               cAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGAAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTGCCTGCCCCAGTGTggcagcagcg                                                                     <  8:245886/149‑6 (MQ=255)                                                                                aaCGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGc                                                                   <  8:49838/149‑1 (MQ=255)                                                                                     gTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGc                                                              >  6:258599/1‑149 (MQ=255)                                                                                           accGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGa                                                        <  6:206403/149‑1 (MQ=255)                                                                                               aGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCtt                                                    >  3:270319/1‑149 (MQ=255)                                                                                                 ggCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGc                                                  >  2:46703/1‑149 (MQ=255)                                                                                                    gaagaaGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGc                                               >  7:33904/1‑149 (MQ=255)                                                                                                     aagaagTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCt                                              >  4:352490/1‑149 (MQ=255)                                                                                                     aagaagTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGGCGACCTTGCCGCt                                              <  1:199038/149‑1 (MQ=255)                                                                                                       gaagTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTgg                                            >  1:31324/1‑149 (MQ=255)                                                                                                              tcCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCaa                                     >  6:353677/1‑149 (MQ=255)                                                                                                                ctgctgGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATg                                   <  4:270319/149‑1 (MQ=255)                                                                                                                 tGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATgg                                  >  4:305278/1‑149 (MQ=255)                                                                                                                  gCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATgg                                  >  8:202900/1‑148 (MQ=255)                                                                                                                         aaTGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCtgtg                          >  6:86545/1‑149 (MQ=255)                                                                                                                         aaTGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGAGGATGGTCGACATCCTCACGCTC‑CTGCCACACTCGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGCCATCt                             >  1:289846/1‑149 (MQ=255)                                                                                                                             cgcgGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCa                       >  4:310706/1‑148 (MQ=255)                                                                                                                               cgGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAg                    <  2:31324/149‑1 (MQ=255)                                                                                                                                   aGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGAt                <  1:46703/149‑1 (MQ=255)                                                                                                                                   aGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGAt                <  5:176544/149‑1 (MQ=255)                                                                                                                                      ttCTACACGTAGC‑GCCAGATTGCCGCGACCTGTTGTTGCAGCTCGTTTCGTT‑‑‑GCTCGACATGCTCACGCTG‑CTGACACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAATGGATGa              <  8:196171/148‑1 (MQ=255)                                                                                                                                      ttCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATc                              <  1:300311/132‑1 (MQ=255)                                                                                                                                      ttCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATc                              >  2:300311/1‑132 (MQ=255)                                                                                                                                      ttCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAg             >  5:354830/1‑149 (MQ=255)                                                                                                                                      ttCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAg             <  3:310706/149‑1 (MQ=255)                                                                                                                                       acAACAGGTCGCGGCCATCTGG‑CGCGACGTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTggg                                           >  3:152443/2‑118 (MQ=37)                                                                                                                                       acAACAGGTCGCGGCCATCTGG‑CGCGACGTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTggg                                           <  4:152443/117‑1 (MQ=37)                                                                                                                                        cAACAGGTCGCGGCCATCTGG‑CGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCt                             <  7:164505/131‑1 (MQ=255)                                                                                                                                        cAACAGGTCGCGGCCATCTGG‑CGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCt                             >  8:164505/1‑131 (MQ=255)                                                                                                                                         aaCACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGtt           <  4:151666/148‑1 (MQ=255)                                                                                                                                         aaCACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTg          >  2:294309/1‑149 (MQ=255)                                                                                                                                            acGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTgcgc       <  2:240094/149‑1 (MQ=255)                                                                                                                                                 gc‑gcCAGATGGCGGCGACCTGTTGGTGCAGCGCGGTGCGTG‑‑‑GCTCGCCTTGCTCTCGGTG‑GTGCCACACTGGGGCTGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCa  <  2:289846/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                        GCTGGCTTCGAACAGGGCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTG‑‑‑GCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCA  >  NC_002947/4826132‑4826424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑