Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,839,707:1 +G 100% coding (73/1443 nt) ccoN‑II → cbb3‑type cytochrome c oxidase subunit 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,839,7071.G82.6% 126.5 / 23.0 46coding (73/1443 nt)ccoN‑IIcbb3‑type cytochrome c oxidase subunit 1
Reads supporting (aligned to +/- strand):  ref base . (4/4);  new base G (15/23);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

GGTAACCTTGCTGGTTATCAATACTGGCGCCGATTTTTCGACCAACAAGAACATCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCT‑GGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGCACACCAACCTGGTGATCTTCGCCTTCGGGGGCTGT  >  NC_002947/4839561‑4839850
                                                                                                                                                   |                                                                                                                                               
gttAACCTTGCTGGTTATCAATACTGGCGCCGATTTTTCGACCAACAAGAAAATCAAAACCCTTGAACCTTAGAATTAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCTACAGTACGCATTCAAGAGGGTGGGCT‑gg                                                                                                                                               >  1:163679/3‑149 (MQ=255)
 gTAACCTTGCTGGTTATCAATACTGGCGCCGATTTTTCGACCAACAAGAACATCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCT‑ggg                                                                                                                                              <  8:159108/149‑1 (MQ=255)
 gTAACCTTGCTGGTTATCAATACTGGCGCCGATTTTTCGACCAACAAGAACATCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCCACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCT‑ggg                                                                                                                                              >  8:86169/1‑149 (MQ=255)
     ccTTGCTGGTTATCAATACTGGCGCCGATTTTTCGACCAACAAGAACATCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCT‑gggggga                                                                                                                                          >  3:116765/1‑147 (MQ=255)
     ccTTGCTGGTTATCAATACTGGCGCCGATTTTTCGACCAACAAGAACATCAAAACCGTGGAACCTTAGAATGAACACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCT‑gggggga                                                                                                                                          >  1:225056/1‑147 (MQ=255)
       ttGCTGGTTATCAATACTGGCGCCGATTTTTCGACCAACAAGAACATCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCT‑ggggggat                                                                                                                                         >  1:248572/1‑145 (MQ=255)
             gTTATCAATACTGGCGCCGATTTTTCGACCAACAAGAACATCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTgg                                                                                                                                    >  7:320621/1‑148 (MQ=255)
                aTCAATACTGGCGCCGATTTTTCGACCAACAAGAACATCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCAt                                                                                                                                 <  5:182192/148‑1 (MQ=255)
                          gcgcCGATTTTTCGACCAACAAGAACATCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGc                                                                                                                       <  7:86169/148‑1 (MQ=255)
                             ccGATTTTTCGACCAACAAGAACATCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCt                                                                                                                   <  5:92564/149‑1 (MQ=255)
                                 tttttCGACCAACAAGAACATCAAAACCGTCGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCAt                                                                                                               <  4:352915/149‑1 (MQ=255)
                                   tttCGACCAACAAGAACATCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTc                                                                                                                 >  6:276906/1‑145 (MQ=255)
                                   tttCGACCAACAAGAACATCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTc                                                                                                                 <  5:276906/145‑1 (MQ=255)
                                       gACCAACAAGAACATCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTc                                                                                                         >  4:251285/1‑149 (MQ=255)
                                                     tCAAATCCGTTGAAACTTAGAATGATCACAGCTATCAGTCCGTCTGGTTATAACTATAAGGTCGTCGGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGGCTCGTCGTCTTCTTCGCCTCGCAGCTGGTGTGGc                                                                                           <  4:116765/149‑1 (MQ=255)
                                                     tCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGc                                                                                           >  1:156115/1‑149 (MQ=255)
                                                     tCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCT‑GGGGGTTCCTTGGCATGGGCCTCGGCGTCt                                                                                                                   <  7:23548/124‑1 (MQ=255)
                                                     tCAAAACCGTGGAACCTTAGAATGAACACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGc                                                                                           <  2:225056/149‑1 (MQ=255)
                                                       aaaaCCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATAGCCTCGCAGCTGGTGTGGCCg                                                                                         >  3:238807/1‑149 (MQ=255)
                                                        aaaCCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACCATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGc                                                                                        <  5:117361/149‑1 (MQ=255)
                                                             gTGGAACCTTAGTATGATCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCTGCATTTCGACATCATGACGGTGGTCTGGGGGGATCGTTTGCATGGGCCTCGGCGTGTTCATCGCCTCGCAGCTGGGGTGGCCGCAGCTg                                                                                   <  2:163679/149‑1 (MQ=255)
                                                                   ccTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTg                                                                             >  8:37819/1‑149 (MQ=255)
                                                                   ccTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCCTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTg                                                                             <  2:279324/149‑1 (MQ=255)
                                                                     ttAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGa                                                                           >  5:317761/1‑149 (MQ=255)
                                                                       aGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGa                                                                           <  7:88498/147‑1 (MQ=255)
                                                                         aaTGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCtt                                                                       <  7:37819/149‑1 (MQ=255)
                                                                             aGCACAGCTAGCAGGCCGGCTGCTTCTAACGATCCGGTCGGCCGCCCGTGCGCGATCATGACGGGGGGCTGGGGGGTTCGTTGGCATGGGCCTCGGCGTCTTCTGCGCCTCGCTGCTGGGGGGGCCGCAGCTGAACCTGGACCTTCCCt                                                                   <  3:192483/149‑1 (MQ=255)
                                                                                     aaTCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGc                                                           >  7:261060/1‑149 (MQ=255)
                                                                                         aGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCg                                                       <  8:261060/149‑1 (MQ=255)
                                                                                          gTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCgg                                                      >  7:92191/1‑149 (MQ=255)
                                                                                               aCTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCT‑GGGGGATCCTTGGCATGGGCCTCGGCGTc                                                                                                                    <  7:321988/81‑1 (MQ=255)
                                                                                               aCTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCT‑GGGGGATCCTTGGCATGGGCCTCGGCGTc                                                                                                                    >  8:321988/1‑81 (MQ=255)
                                                                                                           tataAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGc                                     <  8:92191/149‑1 (MQ=255)
                                                                                                            ataAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGc                                        >  3:110163/1‑145 (MQ=255)
                                                                                                            ataAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGc                                        <  4:110163/145‑1 (MQ=255)
                                                                                                             taAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATgg                                                                                                                               >  4:111376/1‑57 (MQ=21)
                                                                                                             taAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATgg                                                                                                                               <  3:111376/57‑1 (MQ=21)
                                                                                                                  tcgtcCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGCACACCaa                              >  8:6810/1‑149 (MQ=255)
                                                                                                                    gtcCGCCAGTTCGCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGCACACCAAc                             <  6:317761/148‑1 (MQ=255)
                                                                                                                    gtcCGCCAGTTCGCCATCATGACGGTGGTCT‑GGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCT‑GACCAGCTTCGGCCGCCTGCGCCCGCTGCACACCAACCTg                          >  2:316277/1‑149 (MQ=255)
                                                                                                                        gCCAGTTCGCCAACATGACGGTGGTCT‑GGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGCACACCAACCTGGt                        >  1:121206/1‑148 (MQ=255)
                                                                                                                         ccATTTCGCTATAATGACTGTGGTGTGGGGGGATCATTGGAATGGGCCTAGGCGTCTTCATCGCCTAGCTGATGGTGTGGACGGAGCTGAAACTGGATCTTACCTGGACCAGCTTCGGCCGACTGGGCGCTCTGCACTCCTACCTGGTg                       <  4:238807/149‑1 (MQ=255)
                                                                                                                                gCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGCACACCAACCTGGTGATCTTc                 <  2:248572/148‑1 (MQ=255)
                                                                                                                                gCCATCATGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGCACACCAACCTGGTGATCTTc                 <  2:156115/148‑1 (MQ=255)
                                                                                                                                    tcatGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGc                                     <  7:79483/124‑1 (MQ=255)
                                                                                                                                    tcatGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGc                                     >  8:79483/1‑124 (MQ=255)
                                                                                                                                    tcatGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGCACACCAACCTg                          <  2:267759/135‑1 (MQ=255)
                                                                                                                                    tcatGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGCACACCAACCTg                          >  1:267759/1‑135 (MQ=255)
                                                                                                                                    tcatGACGGTGGTCTGGGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGCACACCAACCTGGTGATCTTCGCCtt            >  8:55961/1‑149 (MQ=255)
                                                                                                                                      atGACGGTGGTCT‑GGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGCACACCAACCTGGTGATCTTCGCCTTCgg         >  3:156283/1‑149 (MQ=255)
                                                                                                                                            gtggtCT‑GGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCT‑GACCAGCTTCGGCCGCCTGCGCCCGCTGCACACCAACCTGGTGATCTTCGCCTTCGGGGGCtgt  <  1:316277/149‑1 (MQ=255)
                                                                                                                                             tggtCT‑GGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGCACACCAACCTGGTGATCTTCGCCTTCGGGGGCtgt  <  2:121206/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GGTAACCTTGCTGGTTATCAATACTGGCGCCGATTTTTCGACCAACAAGAACATCAAAACCGTGGAACCTTAGAATGAGCACAGCAATCAGTCCGACTGCTTATAACTATAAGGTCGTCCGCCAGTTCGCCATCATGACGGTGGTCT‑GGGGGATCCTTGGCATGGGCCTCGGCGTCTTCATCGCCTCGCAGCTGGTGTGGCCGCAGCTGAACCTGGACCTTCCCTGGACCAGCTTCGGCCGCCTGCGCCCGCTGCACACCAACCTGGTGATCTTCGCCTTCGGGGGCTGT  >  NC_002947/4839561‑4839850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑