Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	503,837:1	+C	15.2%	intergenic (+40/‑111)	PP_0414 → / → rpe	polyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	503,837	1	.	C	15.2%	145.2 / 24.6	46	intergenic (+40/‑111)	PP_0414/rpe	polyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base . (23/16);  new base C (2/5);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-01

CGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTCCATTC  >  NC_002947/503695‑503978                                                                                                                                                 |                                                                                                                                              cgcgGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGTCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAagag                                                                                                                                         <  6:120423/149‑1 (MQ=255) cgcgGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TGCCGGCCTCTTC‑CGGCTTTAGCCGCGAagag                                                                                                                                         <  7:345938/149‑1 (MQ=39) cgcgGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TGCCGGCCTCTTC‑CGGCTTTAGCCGCGAagag                                                                                                                                         <  5:142168/149‑1 (MQ=39)   cggcggcgACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGc                                                                                                                                       >  5:337911/1‑149 (MQ=255)    ggcggcgACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TGCCGGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGcc                                                                                                                                      <  3:231223/149‑1 (MQ=38)      cggcgACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCAGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCgg                                                                                                                                    <  4:222413/149‑1 (MQ=39)          gaagttGCTGATTGCGTTCTCGGTGTTGTTGCTTCTGACCCTGGAGTGGTTGCGTGGGGTTAGCGAGAAGCTGAGGACTCAACAGCCGGCGTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑TAAGAGGCCGGcaca                                                                                                                                <  6:211093/145‑1 (MQ=255)          gACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TGCCGGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGtaca                                                                                                                                <  7:3514/149‑1 (MQ=38)            ccTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAgg                                                                                                                              <  7:207675/149‑1 (MQ=255)                 cTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCg                                                                                                                         >  4:224358/1‑149 (MQ=255)                    aTTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGt                                                                                                                        <  3:224358/147‑1 (MQ=255)                        cGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc                                                                                                                   <  4:310924/148‑1 (MQ=255)                        cGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc                                                                                                                   >  3:183245/1‑148 (MQ=255)                         gTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGa                                                                                                                 >  5:372183/1‑149 (MQ=255)                            ctcGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGaca                                                                                                               <  6:337911/148‑1 (MQ=255)                             tcGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGacac                                                                                                              >  7:93536/1‑148 (MQ=255)                                              gACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGCCTAAAGCCG‑GAAGAGGCCCGCACAGGTTTCGTTGGTCGACACCAATTGATAAAAATCAg                                                                                             >  4:235197/1‑148 (MQ=255)                                               aCCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGcc                                                                                           >  6:258961/1‑149 (MQ=255)                                                     gAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATc                                                                                     <  6:295167/149‑1 (MQ=255)                                                             gCGTGGGAGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTcc                                                                              <  3:235197/148‑1 (MQ=255)                                                              cGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCt                                                                            <  4:183245/149‑1 (MQ=255)                                                                  ggCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCGTGAg                                                                         >  1:93918/1‑149 (MQ=255)                                                                               gCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGGTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCg                                                                                                                         <  1:29196/87‑1 (MQ=255)                                                                               gCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCg                                                                                                                         >  2:29196/1‑87 (MQ=255)                                                                                     gACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGt                                                      >  4:348465/1‑148 (MQ=255)                                                                                        tCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATGCCAATCAGCCTTCATAGCTTTCCCTGAGTTACAATGCGCGCCACCGTGaag                                                  >  2:14109/1‑148 (MQ=255)                                                                                        tCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAt                                                  >  3:70372/1‑149 (MQ=255)                                                                                        tCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCCTGAAt                                                  >  4:234588/1‑149 (MQ=255)                                                                                          aaCAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACAAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGAGCGCCACCGTGAAttt                                                >  8:196402/1‑149 (MQ=255)                                                                                             aGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGcc                                             <  6:140204/149‑1 (MQ=255)                                                                                                    ttGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGt                                      >  8:213836/1‑149 (MQ=255)                                                                                                            acgcaaccTG‑TGCCGGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTAGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATg                              >  2:116981/7‑149 (MQ=25)                                                                                                                  ccTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTGCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGcc                         <  5:258961/148‑1 (MQ=255)                                                                                                                    tG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGcc                                 >  8:165488/1‑138 (MQ=255)                                                                                                                    tG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGcc                                 <  7:165488/138‑1 (MQ=255)                                                                                                                    tG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTa                      >  8:25060/1‑149 (MQ=255)                                                                                                                        aCCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACg                    <  7:25060/148‑1 (MQ=255)                                                                                                                         ccGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTGTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGc                   >  2:289990/1‑148 (MQ=255)                                                                                                                              ctctTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGc             >  5:37068/1‑149 (MQ=255)                                                                                                                                  ttccgGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGcccc          >  4:184667/4‑149 (MQ=255)                                                                                                                                  tCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGccccc         >  1:248039/1‑149 (MQ=255)                                                                                                                                    gcgGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAttt                                                <  2:97573/108‑1 (MQ=255)                                                                                                                                    gcgGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTc       >  6:162083/1‑149 (MQ=255)                                                                                                                                    gcgGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTc       >  1:18186/1‑149 (MQ=255)                                                                                                                                    gcgGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATAGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAttt                                                >  1:97573/1‑108 (MQ=255)                                                                                                                                           aaGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTCCATtc  <  6:37068/147‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                              CGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTCCATTC  >  NC_002947/503695‑503978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑