Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 684,846:1 +A 7.0% coding (1401/2400 nt) cadA‑II ← cadmium translocating P‑type ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947684,8461.A 7.0% 222.7 / 10.7 57coding (1401/2400 nt)cadA‑IIcadmium translocating P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base . (25/28);  new base A (2/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

GCCGGCCAGGCGGTAAAGGTCGGCGCTGTTGCCGTCCAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTACCCCAGCCAGCCAC  >  NC_002947/684699‑684987
                                                                                                                                                    |                                                                                                                                             
gcCGGCCAGGCGGTAAAGGTCGGCGCTGTTGCCGTCCAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCa                                                                                                                                              <  7:245768/149‑1 (MQ=255)
         gCGGTAAAGGTCGGCGCTGTTGCCGTCCAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGAGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGt                                                                                                                                     <  4:299500/149‑1 (MQ=255)
          cGGTAAAGGTCGGCGCTGTTGCCGTCCAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTg                                                                                                                                    <  1:250946/149‑1 (MQ=255)
          cGGTAAAGGTCGGCGCTGTTGCCGTCCAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTg                                                                                                                                    <  5:174645/149‑1 (MQ=255)
                            ttGCCGTCCAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCgg                                                                                                                   >  1:254155/1‑148 (MQ=255)
                                    cAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCa                                                                                                          <  2:122096/149‑1 (MQ=255)
                                    cAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCa                                                                                                          >  5:103220/1‑149 (MQ=255)
                                      gcgcCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCCCGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCAt                                                                                                         >  2:266396/1‑148 (MQ=255)
                                      gcgcCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCAt                                                                                                         >  3:12436/1‑148 (MQ=255)
                                       cgcCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGa                                                                                                       <  6:103220/149‑1 (MQ=255)
                                         ccTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATc                                                                                                     <  5:204932/149‑1 (MQ=255)
                                             gCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCgcgg                                                                                                 >  8:214368/1‑149 (MQ=255)
                                                 tgAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGcggcgg                                                                                              <  8:268541/148‑1 (MQ=255)
                                                 tgAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCggcggg                                                                                             >  3:306363/1‑149 (MQ=255)
                                                   aaCCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCggcgggc                                                                                            <  2:205327/148‑1 (MQ=255)
                                                    accaccTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGgtgt                                                                                          <  4:12436/149‑1 (MQ=255)
                                                    accaccTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGt                                                                                          >  4:289774/1‑149 (MQ=255)
                                                      caccTGCGGGCTGCCGGAGGTGAGGGTGCAGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCCTCGGCATCCTTGATCAGGATGCCGTGCCGGGCGGCGACCCCGGAACCGGCCATCATCGCGGCGGCCGTgg                                                                                        >  7:97112/1‑149 (MQ=255)
                                                        ccTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGc                                                                                       >  1:145231/1‑148 (MQ=255)
                                                         cTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCGGTGGCGGGCGGCGACCCCg                                                                                                                    >  2:311469/1‑118 (MQ=255)
                                                         cTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCg                                                                                                                    <  1:311469/118‑1 (MQ=255)
                                                            cGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAgg                                                                                   <  2:254155/148‑1 (MQ=255)
                                                             gggCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGcc                                                                                 >  8:282798/1‑149 (MQ=255)
                                                             gggCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGcc                                                                                 <  4:30208/149‑1 (MQ=255)
                                                               gCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGa                                                                               <  5:60534/149‑1 (MQ=255)
                                                                cTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAg                                                                              <  5:37402/149‑1 (MQ=255)
                                                                  gCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAggg                                                                            <  2:283063/149‑1 (MQ=255)
                                                                      gAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCa                                                                        <  2:145231/149‑1 (MQ=255)
                                                                         gTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAggg                                                                     <  7:236015/149‑1 (MQ=255)
                                                                           gAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAgggg                                                                    >  3:71060/1‑148 (MQ=255)
                                                                              ggTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAg                                                                 <  4:71060/148‑1 (MQ=255)
                                                                                  ccGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGAt                                                            <  7:282798/149‑1 (MQ=255)
                                                                                    ggTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGcggcgg                                                                                              >  4:195314/1‑113 (MQ=255)
                                                                                    ggTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGcggcgg                                                                                              <  3:195314/113‑1 (MQ=255)
                                                                                             gAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCacgg                                                 >  8:1069/1‑149 (MQ=255)
                                                                                                   tACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCacggcgacgg                                           >  6:4161/1‑149 (MQ=255)
                                                                                                     cgcgGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCacggcgacggcg                                         >  2:351537/1‑149 (MQ=255)
                                                                                                     cgcgGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCacggcgacggcg                                         >  2:30917/1‑149 (MQ=255)
                                                                                                             aCCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTggcgggcg                                                                                                                             >  1:269875/1‑57 (MQ=255)
                                                                                                             aCCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTggcgggcg                                                                                                                             <  2:269875/57‑1 (MQ=255)
                                                                                                             aCCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAg                                 >  4:159813/1‑149 (MQ=255)
                                                                                                              ccGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAgc                                <  3:289774/149‑1 (MQ=255)
                                                                                                               cGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAgcg                               >  8:43226/1‑149 (MQ=255)
                                                                                                                 cATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGcgcg                             >  8:253863/1‑149 (MQ=255)
                                                                                                                              cAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCgg                  <  1:351537/147‑1 (MQ=255)
                                                                                                                              cAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTa                <  7:253863/149‑1 (MQ=255)
                                                                                                                              cAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTa                <  7:214368/149‑1 (MQ=255)
                                                                                                                               aGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTAc               >  2:56374/1‑149 (MQ=255)
                                                                                                                               aGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTAc               <  7:1069/149‑1 (MQ=255)
                                                                                                                                gggCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGTGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTAcc              <  1:266396/149‑1 (MQ=255)
                                                                                                                                   cTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTAccc             >  6:112773/1‑147 (MQ=255)
                                                                                                                                      cGGCATCCTGATCAAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTACCccagc         >  5:222133/1‑148 (MQ=255)
                                                                                                                                       ggCATCCTGATCAAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCacggcgacggcg                                         >  1:70295/1‑115 (MQ=255)
                                                                                                                                       ggCATCCTGATCAAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCacggcgacggcg                                         <  2:70295/115‑1 (MQ=255)
                                                                                                                                       ggCATCCTGATCAAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTACCccagcca       <  6:222133/149‑1 (MQ=255)
                                                                                                                                        gCATGCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTACCccagccag      >  6:132520/1‑149 (MQ=255)
                                                                                                                                         cATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTACCCcagccagc     <  2:62863/149‑1 (MQ=255)
                                                                                                                                            ccTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTACCCCAGccagccac  >  4:316170/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
GCCGGCCAGGCGGTAAAGGTCGGCGCTGTTGCCGTCCAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATCAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTACCCCAGCCAGCCAC  >  NC_002947/684699‑684987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: