Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,419,548:1 +C 100% intergenic (‑37/+99) PP_1242 ← / ← PP_1244 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,419,5481.C92.9% 127.2 / 3.5 42intergenic (‑37/+99)PP_1242/PP_1244hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base C (20/19);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑C‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAAT  >  NC_002947/1419401‑1419678
                                                                                                                                                    |                                                                                                                                   
cTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                                    <  2:94962/149‑1 (MQ=255)
       gggATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTcg                                                                                                                             >  4:40766/1‑149 (MQ=255)
                ggTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGc                                                                                                                    <  3:102639/149‑1 (MQ=255)
                     gATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCt                                                                                                               >  3:34866/1‑149 (MQ=255)
                      aTGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGc                                                                                                                    >  7:157783/1‑143 (MQ=255)
                      aTGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGc                                                                                                                    <  8:157783/143‑1 (MQ=255)
                         cGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTccc                                                                                                            <  8:128108/148‑1 (MQ=255)
                              cAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAgg                                                                                                       >  7:285315/1‑148 (MQ=255)
                                   ggTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACtgt                                                                                                 <  8:197953/149‑1 (MQ=255)
                                    gTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAgggtcacca                                                                                                <  5:182364/149‑8 (MQ=255)
                                      gAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGACGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCACTGGATTCTGGGTTGCATGGGATGG‑CATCTTCGCGTGCAAGCCCGCTCCCACAGGTACtgtg                                                                                                >  8:249322/1‑147 (MQ=255)
                                       aGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCa                                                                                              <  2:25324/148‑1 (MQ=255)
                                       aGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCa                                                                                              <  4:34866/148‑1 (MQ=255)
                                         tgttgtTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACtgt                                                                                                 <  2:44607/143‑1 (MQ=255)
                                         tgttgtTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACtgt                                                                                                 >  1:44607/1‑143 (MQ=255)
                                         tgttgtTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTg                                                                                           <  3:40766/149‑1 (MQ=255)
                                             gttTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGTGCACGCCCGCTCCAACAGGTACAGTACAGTGCGt                                                                                        >  4:243942/1‑148 (MQ=255)
                                             gttTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACCCCCCCTCCCACCGGTACTGTGCAGTGCGTg                                                                                       >  7:195805/1‑149 (MQ=255)
                                              ttttCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGa                                                                                      >  6:278119/1‑149 (MQ=255)
                                               tttCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGAc                                                                                     >  6:349051/1‑149 (MQ=255)
                                                                catttCTTTCCTACTGTTGTTGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGTTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCtgt                                                                    <  7:249322/145‑1 (MQ=38)
                                                                cAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCtgt                                                                    <  5:278119/149‑1 (MQ=255)
                                                                 aGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGACTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGAc                                                                                     <  8:204931/131‑1 (MQ=255)
                                                                 aGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGACTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGAc                                                                                     >  7:204931/1‑131 (MQ=255)
                                                                   gTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCgg                                                                                                                          >  5:329435/1‑92 (MQ=255)
                                                                   gTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCgg                                                                                                                          <  6:329435/92‑1 (MQ=255)
                                                                   gTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTggg                                                                 >  3:223525/1‑149 (MQ=255)
                                                                      ttGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TATTAGAGGGCCCGCCCCCTACCCCAAGGTCAGTTGAGTGGGTTTCGGCTGTGGGGACCCAGTGGGGGc                                                              >  8:175814/1‑149 (MQ=255)
                                                                         ccGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCgg                                                            <  5:349051/148‑1 (MQ=255)
                                                                                            ttgtgttgtCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCggg                                                           >  2:308721/1‑130 (MQ=255)
                                                                                            ttgtgttgtCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCggg                                                           <  1:308721/130‑1 (MQ=255)
                                                                                            ttgtgttgtCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGCCACGCCCGCTCCCACAGGTACTGTGCAGTGCGGGACGGCTGTGGTGACCCTGGGGGAGCGGGCGTGCCCGCGAAGAGGc                                          <  8:285315/147‑1 (MQ=38)
                                                                                                       gTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGAGGGCTGTGGTGCCCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAAt                             <  2:302724/149‑1 (MQ=255)
                                                                                                       gTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGAGTGCCCGCGAAGAGGCCTGACCTGCTAAt                             >  5:262239/1‑149 (MQ=255)
                                                                                                           gCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGc                           >  6:354332/1‑147 (MQ=255)
                                                                                                           gCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGc                           >  1:237113/1‑147 (MQ=255)
                                                                                                                      tttCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTAGTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCggg                                                           <  2:334629/104‑1 (MQ=255)
                                                                                                                      tttCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCggg                                                           >  1:334629/1‑104 (MQ=255)
                                                                                                                           cTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCTGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCtgtg         >  5:309426/1‑149 (MQ=255)
                                                                                                                             gttttgtGGGTGGCGTGGGGTGG‑CGTCTGGGCGGGCACGCCGGTTCCCCATGTTAGTGTGCTGTGCTTGCCGGCTGTGGTGACCGTGTGGTTGGGGGGGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTgg        <  4:223525/142‑1 (MQ=255)
                                                                                                                                   tGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCtgt                                                                    <  2:218022/82‑1 (MQ=255)
                                                                                                                                   tGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCtgt                                                                    >  1:218022/1‑82 (MQ=255)
                                                                                                                                     ggTTGCCGGGGCTGG‑CATCTTCGCGGGCACGCCCGCTCCCACCGGTACTGTGCAGTGCGTGACGGCTGTGTTGACCCTGTGGGTGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAAt  <  3:243942/146‑1 (MQ=37)
                                                                                                                                                    |                                                                                                                                   
CTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑C‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAAT  >  NC_002947/1419401‑1419678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑