| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,932,638:1 | +C | 100% | intergenic (‑105/+45) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 94.1% | 104.8 / ‑0.0 | 34 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (15/17); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTT > NC_002947/1932506‑1932781 | agagGATCTGGACATTCGACAGCGCCATTGCAGGTCTCACTAAAAAAGCAGTAAACGCTGGGGCCGCTTTGGGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCTAAGGGCt < 4:300156/146‑1 (MQ=38) ggATCTGGACATTCGACCGCGCAATTCCAGGTCTCACTAAAATACCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCACTCCTACAAAGGATATGTGTCCCCTGTAGTAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAgg < 8:142034/149‑1 (MQ=37) agccggccATTCGCCACCGCCATAGCAGGCCGCCCCAAAATCCCACTAAACGCCGGGGCCGCATTGCGGCCCGGCGCGGCCACGCCCGCTCCTCCACAGGAAACGCGTCCCCTGTAGCAGC‑GGGCTG‑GCCCGCGAAGGGCTGCAAGGCa < 8:244270/142‑1 (MQ=255) aTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCATACGCGGCCAAGCCCGATCCAAAAAAGGAATTGCGTCCCCTGTAGGACC‑GGGGTT‑GCCCCCGAAGGGCGGAAAGGCAGCCCCaaa > 1:217592/1‑149 (MQ=255) aCAGCGGTATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGTATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 2:70085/149‑1 (MQ=255) aGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAATCCCGCTACAACAAAGGATATGCGTACCCTGTAGGAGC‑GGGCTT‑GCCCGCCAAGGGCTGCAACGCAGCCCCAAATTGtag > 4:239650/1‑146 (MQ=37) aGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGAt > 2:67441/1‑148 (MQ=255) gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTGCGACAAAGGATATGCGGCCCCGGGAGGAGCGGGGGTG‑G‑CCGCGCAGGGCTGCAAGGGCGCCCCCAAATTTGGCCTACGCGGGTCAg > 7:315630/1‑149 (MQ=25) tcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGGTTGG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAAATGTc > 1:132043/1‑131 (MQ=255) aaTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGCATATGCGGCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCa < 3:239650/149‑1 (MQ=255) gccctAACCGCTGGGGCCGCTTTGCGGCCCTCCGCGGCCAACCCCGCTCCAACAAAGGAAATGCGTCCCCTGTAGGAGC‑GGCCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGt < 8:342926/145‑1 (MQ=38) cAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTTTAGACTACGCCGATCAGCGATCCGGCAGGGt > 6:109313/1‑148 (MQ=37) aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGtt > 3:309110/1‑149 (MQ=255) tGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGCCTACGCGGATCAGCGCTCCGGCAGGGTGATGTTCAGCt > 3:115943/1‑149 (MQ=255) tGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAg > 1:54477/1‑147 (MQ=255) tGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAg < 2:54477/147‑1 (MQ=255) ggggCCGCTTGCGGGCCGTGCGCGGCCAACCCGGCTCATACAATGTATATGCGTCCCGTTTCGGAGG‑GGGTTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTc < 3:358435/149‑1 (MQ=255) ccGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAg < 7:346584/130‑1 (MQ=255) ccGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAAGTGTCGACTACGCGGATCAGCGATCCGGCAg > 8:346584/1‑130 (MQ=255) cgtgttGCGGCCCTGCGCGGGCACGCCCGCTCCAACAACGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGaa < 8:5339/145‑1 (MQ=38) gcgGGCAAGCCCGCTCCTACAACGGATTTGCGTCCCCTGTCGGAGC‑GGGCTG‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt < 4:115943/148‑1 (MQ=39) gcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGTGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTg < 6:127188/149‑1 (MQ=255) gcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt < 2:217592/148‑1 (MQ=255) cgCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc < 5:109313/148‑1 (MQ=255) cGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCt > 2:15815/1‑149 (MQ=255) cGCTCCTAAAAAGTATATGCGGCCCCTGTAGCAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCt < 8:315630/149‑1 (MQ=39) tCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGa > 5:164986/1‑149 (MQ=255) gCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt > 6:352701/1‑149 (MQ=255) tCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTTCAAGGGAGCCCCAAATTTTCGCCGAAGCGGATCAGCGGTGCGGCAGGGGGATGTTGAGGTGCCGAATCGAGGCGCGGCCCTGGGTTTGCACCTCGGTATGCACAAGATCGTTgg > 4:8331/1‑148 (MQ=255) tCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc > 3:152052/1‑149 (MQ=255) cTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt > 1:24555/1‑149 (MQ=255) ggAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 4:121880/148‑1 (MQ=255) gC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACat < 4:152052/149‑1 (MQ=255) gCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAttt > 3:177023/1‑149 (MQ=255) | AGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTT > NC_002947/1932506‑1932781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |