Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,941,177:1 +C 100% coding (100/1578 nt) PP_1740 ← beta (1‑6) glucans synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,941,1771.C87.5% 115.5 / 11.4 40coding (100/1578 nt)PP_1740beta (1‑6) glucans synthase
Reads supporting (aligned to +/- strand):  ref base . (3/2);  new base C (18/17);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

CCATCCGCGCTGGGCGCAGGCGAAACGGCTGGTCGTAAGGCGACTGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAG‑‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTGGCACAAAAGGTTAGATAGTAGCCCGGCGCCAAACCTTTGGCATG  >  NC_002947/1941036‑1941314
                                                                                                                                              |                                                                                                                                           
ccATCCGCGCTGGGCGCAGGCGAAACGGCTGGTCGTAAGGCGACTGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAG‑‑c‑cccccc                                                                                                                                    <  6:156729/149‑1 (MQ=255)
  aTCCGCGCTGGGCGCAGGCGAAACGGCTGGTCGTAAGGCGACTGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAG‑‑c‑cccccc                                                                                                                                    >  3:85379/1‑147 (MQ=255)
        gcTGGGCGCAGGCGAAACGGCTGGTCGTAAGGCGACTGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGa                                                                                                                             >  8:331112/1‑149 (MQ=255)
             gcgcAGGCGAAACGGCTGGTCGTAAGGCGACTGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAgg                                                                                                                        >  6:68156/1‑149 (MQ=255)
              cgcAGGCGAAACGGCTGGTCGTAAGGCGACTGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAggg                                                                                                                       <  1:267871/149‑1 (MQ=255)
                     gAAACGGCTGGTCGTAAGGCGACTGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGCCC‑CCCCCCAGCCCGACCAGGGCcagca                                                                                                                 <  1:82274/149‑1 (MQ=255)
                             tGGTCGTAAGGCGACTGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCaggcaggc                                                                                                          >  3:241960/1‑147 (MQ=255)
                                         gACTGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACg                                                                                            <  5:68156/149‑1 (MQ=255)
                                          aCTGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACgg                                                                                           <  7:331112/149‑1 (MQ=255)
                                           cTGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACgg                                                                                           >  5:171892/1‑148 (MQ=255)
                                           cTGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGc                                                                                          <  6:65439/149‑1 (MQ=255)
                                            tGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGc                                                                                          >  3:278865/1‑148 (MQ=255)
                                            tGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGGGTGCAGGGCTGGCGGCATCGGGCAGGGGCACTGGCTTGCCCAGCCGGTACCCGAG‑‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCaa                                                                                        <  1:167772/149‑1 (MQ=255)
                                               tCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGCGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGGCGGTACACCGGCAAt                                                                                       >  4:70125/1‑148 (MQ=255)
                                               tCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAAt                                                                                       >  3:235502/1‑148 (MQ=255)
                                                cTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATaa                                                                                     >  6:34222/1‑149 (MQ=255)
                                                 tttGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAG‑‑CTCCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATaa                                                                                     >  6:273687/1‑148 (MQ=255)
                                                  ttGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAG‑‑C‑CCCCCCAGACCGACAAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAg                                                                                  >  5:64894/1‑149 (MQ=255)
                                                     tCGAACGTGGTGTACGAAGGGGATTGCAGGTTGTGTGCAGTGCTGGCCGCATCGGGCAGGTGCACTGGCTTGGCCAGCCGGTACGAGAG‑‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGTCTAGCAGGTACAACGGCAATTACAggg                                                                                <  6:64894/148‑2 (MQ=255)
                                                       gAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAggcgg                                                                              >  3:73599/1‑149 (MQ=255)
                                                          cGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTa                                                                           <  4:73599/149‑1 (MQ=255)
                                                           ggCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTAc                                                                          <  1:83574/149‑1 (MQ=255)
                                                                         gcATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTgg                                                            <  4:241960/149‑1 (MQ=255)
                                                                           aTTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCAAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCg                                                          >  5:148002/1‑149 (MQ=255)
                                                                              gCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGt                                                        <  4:278865/148‑1 (MQ=255)
                                                                               cAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTc                                                       >  6:3088/1‑148 (MQ=255)
                                                                                        tgCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTg                                              <  4:235502/148‑1 (MQ=255)
                                                                                               cTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTGGCACaaaa                                      <  5:34222/149‑1 (MQ=255)
                                                                                                   ccGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTGGCACAAAAGGt                                   >  3:163494/1‑148 (MQ=255)
                                                                                                    cGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTGGCACAAAAGGtt                                  >  7:91324/1‑148 (MQ=255)
                                                                                                    cGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTGGCACAAAAGGtt                                  <  8:91324/148‑1 (MQ=255)
                                                                                                     gCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTGGCACAAAAGGTTAg                                <  6:171892/149‑1 (MQ=255)
                                                                                                                    acaGGCTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTGGCACAAAAGGTTAGATAGTAGCCCGgcgc                 >  7:37304/1‑149 (MQ=255)
                                                                                                                     caGGCTTGCCCAGCCCGTACCAGAGCCC‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTGGCACAAAAGGTTAGATAGTAGCCCGgcgc                 >  4:341072/1‑149 (MQ=255)
                                                                                                                     caGGCTTGCCCAGCCCGTACCAGAGCCC‑CCCCCCAGCCCGACCAGGCCCAGCACGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGAGGCGGTCGAACGTCTGGCACAAAAGCTTAGAAAGTAGCCcgccgc                 >  2:38228/1‑149 (MQ=255)
                                                                                                                         cTTGCCCAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTGGCACAAAAGGTTAGATAGTAGCCCGGCGCCAAAc            <  6:148002/149‑1 (MQ=255)
                                                                                                                          ttGCCCAGCCCGTACCAGAG‑‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTGGCACAAAAGGTTAGATAGTAGCCCGGCGCCAAACCt          >  3:47706/1‑149 (MQ=255)
                                                                                                                             cccAGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTGGCACAAAAGGTTAGATAGTAGCCCGGCGCCAAACCttt        <  4:85379/149‑1 (MQ=255)
                                                                                                                                aGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCgg                                                         >  4:294552/1‑97 (MQ=255)
                                                                                                                                aGCCCGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCgg                                                         <  3:294552/97‑1 (MQ=255)
                                                                                                                                   ccGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTGGCACAAAAGGTTAGATAGTAGCCCGGCGCCAAACCTTTGGCATg  <  6:167672/149‑1 (MQ=255)
                                                                                                                                   ccGTACCAGAGC‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTGGCACAAAAGGTTAGATAGTAGCCCGGCGCCAAACCTTTGGCATg  >  5:363046/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                           
CCATCCGCGCTGGGCGCAGGCGAAACGGCTGGTCGTAAGGCGACTGGTCTTTGTCGAACGGCGTGTACGAAGCGCATTGCAGCTTGTGTGCAGGGCTGGCCGCATCGGGCAGGTGCACAGGCTTGCCCAGCCCGTACCAGAG‑‑C‑CCCCCCAGCCCGACCAGGGCCAGCAGGCAGGCTAGCAGGTACAACGGCAATAACAGGCGGCTACAACTGGGCATGTGGCGGTCCATCGTCTGGCACAAAAGGTTAGATAGTAGCCCGGCGCCAAACCTTTGGCATG  >  NC_002947/1941036‑1941314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑