Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,682,906:1 +C 9.4% coding (1472/1890 nt) prpE → propionyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,682,9061.C 9.4% 193.9 / 13.7 53coding (1472/1890 nt)prpEpropionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base . (25/23);  new base C (2/3);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

ATTGCCCTGCCATTGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAG  >  NC_002947/2682761‑2683039
                                                                                                                                                  |                                                                                                                                     
aTTGCCCTGCCATTGCCACCCGGTTGCAGTCAGACGCTGTTGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑g                                                                                                                                      <  5:142066/147‑1 (MQ=255)
aTTGCCCTGCCATTGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGTGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑ggg                                                                                                                                    <  7:164736/149‑1 (MQ=255)
aTTGCCCTGCCATTGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑g                                                                                                                                      >  6:142066/1‑147 (MQ=255)
 ttGCCCTGCCATTGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑gggg                                                                                                                                   <  7:344691/149‑1 (MQ=255)
 ttGCCCTGCCATTGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑gggg                                                                                                                                   <  7:258176/149‑1 (MQ=255)
    cccTGCCATTGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGgcg                                                                                                                                 >  1:316969/1‑148 (MQ=255)
    cccTGCCATTGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGgcg                                                                                                                                 <  7:217413/148‑1 (MQ=255)
      cTGCCATTGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCAc                                                                                                                              >  3:28462/1‑149 (MQ=255)
            ttGCCCCCCGGTTGCAGTCAGACGCTGTGGGGGGATCCCGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTGCGTCTACATCAT‑GGGGCGCACCgatga                                                                                                                        >  5:183211/1‑149 (MQ=255)
            ttGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCgatga                                                                                                                        >  4:96041/1‑149 (MQ=255)
            ttGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCgatga                                                                                                                        <  4:101980/149‑1 (MQ=255)
            ttGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCgatga                                                                                                                        >  3:310994/1‑149 (MQ=255)
                cACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACATGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCTCACCGATGAtgt                                                                                                                     >  4:349616/1‑148 (MQ=255)
                     ggTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCATCGGTGCGC‑CCCATGAtgtagacga                                                                                                               <  7:68675/149‑7 (MQ=255)
                     ggTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCaa                                                                                                               <  4:28462/149‑1 (MQ=255)
                        tGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGt                                                                                                            >  4:130062/1‑149 (MQ=255)
                           aGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCATCGGTGCGC‑CCCATGAtgt                                                                                                                     >  2:32059/1‑137 (MQ=255)
                           aGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTCCGTCTACATCATCGGTGCGC‑CCCATGAtgt                                                                                                                     <  1:32059/137‑1 (MQ=255)
                            gTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGTCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTcc                                                                                                        <  2:316969/149‑1 (MQ=255)
                                     tgtgGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGt                                                                                               >  5:254721/1‑149 (MQ=255)
                                       tgGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGt                                                                                               <  4:310994/147‑1 (MQ=255)
                                         gggggATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGt                                                                                           >  8:15313/1‑149 (MQ=255)
                                            ggATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCa                                                                                        >  1:163086/1‑149 (MQ=255)
                                                        cTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCATCGGTGCGC‑CCCATGAtgta                                                                                                                    >  4:186357/1‑108 (MQ=255)
                                                        cTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCATCGGTGCGC‑CCCATGAtgta                                                                                                                    <  3:186357/109‑2 (MQ=255)
                                                         tACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATgg                                                                            >  4:331732/1‑148 (MQ=255)
                                                            cTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGa                                                                        >  5:39464/1‑149 (MQ=255)
                                                              gCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGAcc                                                                      >  3:47784/1‑149 (MQ=255)
                                                              gCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGAcc                                                                      >  1:54548/1‑149 (MQ=255)
                                                                aGGCCTACCTGCTCACGTATCCTGTGTACTACCAGACGGGCTATGGCGGCTATCTGTCAGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCt                                                                     <  3:349616/148‑1 (MQ=255)
                                                                aGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTg                                                                    <  3:96041/149‑1 (MQ=255)
                                                                     tACCTGCGCACGTATCCGGGGTACTACCCCACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTcgc                                                               >  4:331023/1‑149 (MQ=255)
                                                                            gcACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCAc                                                        >  4:58605/1‑149 (MQ=255)
                                                                              aCGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCAccc                                                      >  1:66111/1‑149 (MQ=255)
                                                                              aCGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCAccc                                                      >  8:6407/1‑149 (MQ=255)
                                                                                     cGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATgg                                                                            >  2:318409/1‑120 (MQ=255)
                                                                                     cGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATgg                                                                            <  1:318409/120‑1 (MQ=255)
                                                                                       gggTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGc                                             <  3:130062/149‑1 (MQ=255)
                                                                                       gggTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGc                                             <  7:15313/149‑1 (MQ=255)
                                                                                        ggTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCg                                            <  5:189146/149‑1 (MQ=255)
                                                                                            cttccACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAgt                                        <  6:254721/146‑1 (MQ=255)
                                                                                              acCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAgtgt                                      >  4:120684/1‑149 (MQ=255)
                                                                                               ccACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGa                                          <  4:277153/144‑1 (MQ=255)
                                                                                               ccACACGGGCGATGGCGGCTAGCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGa                                          >  3:277153/1‑144 (MQ=255)
                                                                                                 acacGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAgt                                        >  1:286008/1‑144 (MQ=255)
                                                                                                 acacGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAgt                                        <  2:286008/144‑1 (MQ=255)
                                                                                                  cacGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGGCACCCGGCGGTAGCGGAGTTTGCtg                                  <  2:66111/149‑1 (MQ=255)
                                                                                                  cacGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCtg                                  <  2:54548/149‑1 (MQ=255)
                                                                                                   acGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCcggcg                                                  >  2:257341/1‑132 (MQ=255)
                                                                                                   acGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGATTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCcggcg                                                  <  1:257341/132‑1 (MQ=255)
                                                                                                          aTGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGgt                          <  7:6407/149‑1 (MQ=255)
                                                                                                            ggcggcTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGgtg                         <  6:39464/148‑1 (MQ=255)
                                                                                                                gcTATCTTGACTATGATGTTTTCGTCTACATCAT‑GGTGCGGACCGATTATGTGATCTACGTTTCCCGTCATCGTTTGTCCACCGGTTAAATGGAAGACCTGGTCGCCCGCCAACCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCAt                    <  7:95529/149‑1 (MQ=255)
                                                                                                                           gatgatGGTTTCGTCTACATCAT‑GGGGCGGACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCaa         <  3:120684/149‑1 (MQ=255)
                                                                                                                           gatgatGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCaa         <  3:331023/149‑1 (MQ=255)
                                                                                                                              gatgGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCGACCGGTGATATGGAAGAGCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAggg      >  2:223101/1‑149 (MQ=255)
                                                                                                                               atgGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGc     >  4:489/1‑149 (MQ=255)
                                                                                                                                  gTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAg  <  3:58605/149‑1 (MQ=255)
                                                                                                                                             atcat‑GGGGCGCACCGATGATGTGATCAACGTTCCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGgtg                         <  2:82873/115‑1 (MQ=255)
                                                                                                                                             atcat‑GGGGCGCACCGATGATGTGATCAACGTTACCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGgtg                         >  1:82873/1‑115 (MQ=255)
                                                                                                                                                  |                                                                                                                                     
ATTGCCCTGCCATTGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAG  >  NC_002947/2682761‑2683039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑