Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,845,979:1 +T 8.0% coding (137/330 nt) PP_2497 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,845,9791.T 8.0% 188.3 / 11.0 50coding (137/330 nt)PP_2497hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (23/23);  new base T (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

CCAGGACATGACGGTCCAGCGACTCGCCGAGCTGCGCGCCAATCGCACAACCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTGGGGAGTGACAGCGGGGGCACTGCAATGACATGGGGCGAA  >  NC_002947/2845836‑2846123
                                                                                                                                                |                                                                                                                                                
ccAGGACATGACGGTCCAGCGACTCGCCGAGCTGCGCGCCAATCGCACAACCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCG‑GTGCAGCACTCGGcc                                                                                                                                             <  7:46200/149‑1 (MQ=255)
ccAGGACATGACGGTCCAGCGACTCGCCGAGCTGCGCGCCAATCGCACAACCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCG‑GTGCAGCACTCGGcc                                                                                                                                             <  7:51446/149‑1 (MQ=255)
  aGGACATGACGGTCCAGCGACTCGCCGAGCTGCGCGCCAATCGCACAACCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAc                                                                                                                                                   <  8:144674/142‑1 (MQ=255)
  aGGACATGACGGTCCAGCGACTCGCCGAGCTGCGCGCCAATCGCACAACCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAc                                                                                                                                                   >  7:144674/1‑142 (MQ=255)
    gACATGACGGTCCAGCGACTCGCCGAGCTGCGCGCCAATCGCACAACCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGTCCGAGTGCTGCAC‑CGGCCTTgc                                                                                                                                         <  3:126666/149‑1 (MQ=255)
    gACATGACGGTCCAGCGACTCGCCGAGCTGCGCGCCAATCGCACAACCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTgc                                                                                                                                         <  4:192634/149‑1 (MQ=255)
              tCCAGCGACTCGCCGAGCTGCGCGCCAATCGCACAACCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGc                                                                                                                                  >  3:30965/1‑146 (MQ=255)
                cAGCGACTCGCCGAGCTGCGCGCCAATCGCACAACCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCagag                                                                                                                             >  2:95436/1‑149 (MQ=255)
                                      ccAATCGCACAACCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAg                                                                                                       <  3:345390/149‑1 (MQ=255)
                                          tCGCACAACCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGAGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCt                                                                                                   <  7:132716/149‑1 (MQ=255)
                                             cacaACCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGagca                                                                                                                           >  6:361857/1‑122 (MQ=255)
                                             cacaACCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGagca                                                                                                                           <  5:361857/122‑1 (MQ=255)
                                                aaCCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCagag                                                                                                                             >  2:324055/1‑117 (MQ=255)
                                                aaCCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCagag                                                                                                                             <  1:324055/117‑1 (MQ=255)
                                                aaCCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACa                                                                                             <  5:218705/149‑1 (MQ=255)
                                                    accGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAgcgc                                                                                         >  2:132753/1‑149 (MQ=255)
                                                    accGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAgcgc                                                                                         >  5:106700/1‑149 (MQ=255)
                                                      cGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGcc                                                                                        >  8:123894/1‑148 (MQ=255)
                                                               cGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGACGAGTGCTGCCC‑CGGCCTTGCCGCCTGCCAGCGCAGCGATGACGCCCCGTGCGAGGCCTCCGCCAGCGCCGATGGCcgcg                                                                              >  7:340924/1‑149 (MQ=255)
                                                                ggCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCg                                                                                                         >  3:315937/1‑121 (MQ=255)
                                                                ggCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCg                                                                                                         <  4:315937/121‑1 (MQ=255)
                                                                 gCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGcc                                                                            >  1:70586/1‑149 (MQ=255)
                                                                       ccgccgAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCttt                                                                      <  1:95436/149‑1 (MQ=255)
                                                                           cgAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATg                                                                                    <  1:259518/131‑1 (MQ=255)
                                                                           cgAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATg                                                                                    >  2:259518/1‑131 (MQ=255)
                                                                               aaTGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCg                                                               >  2:350217/1‑148 (MQ=255)
                                                                               aaTGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCg                                                               <  1:350217/148‑1 (MQ=255)
                                                                                   gcgcCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATg                                                          <  2:176516/149‑1 (MQ=255)
                                                                                        ggATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATg                                                          <  8:143349/144‑1 (MQ=255)
                                                                                        ggATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATg                                                          >  7:143349/1‑144 (MQ=255)
                                                                                          aTACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGa                                                    >  8:119713/1‑148 (MQ=255)
                                                                                           tACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGaa                                                   <  7:119713/148‑1 (MQ=255)
                                                                                              cgagccgagAGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGa                                               >  3:200786/1‑149 (MQ=255)
                                                                                               gagccgagGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGAc                                              <  6:106700/149‑1 (MQ=255)
                                                                                                    gagGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGCCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTg                                         >  7:210149/1‑149 (MQ=255)
                                                                                                       ggTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTggg                                       <  7:123894/148‑1 (MQ=255)
                                                                                                        gTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTggg                                       >  6:91304/1‑147 (MQ=255)
                                                                                                                gACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTGGGGAGTGACAGc                             >  5:105101/1‑149 (MQ=255)
                                                                                                                 aCAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTGGGGAGTGACAGCg                            <  6:105101/149‑1 (MQ=255)
                                                                                                                       ccGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTGGGGAGTGa                                 >  3:1656/1‑138 (MQ=255)
                                                                                                                       ccGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATCAAGCGACTTTTGGGGAGTGa                                 <  4:1656/138‑1 (MQ=255)
                                                                                                                        cGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTGGGGAGTGACAGCGGGGGCAc                     >  8:215735/1‑149 (MQ=255)
                                                                                                                         ggCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTGGGGAGTGACAGCGGGGGCACt                    <  5:91304/149‑1 (MQ=255)
                                                                                                                           cggcaaGGCCG‑GTGCAGCACTCGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTGGGGAGTGACAGCGGGGGCACTg                   >  5:269549/6‑148 (MQ=255)
                                                                                                                            gATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTggg                                       >  8:46956/1‑127 (MQ=255)
                                                                                                                            gATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTggg                                       <  7:46956/127‑1 (MQ=255)
                                                                                                                              caaGGCCG‑GTGCAGCACTCGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTGGGGAGTGACAGCGGGGGCACTGCAAt               >  5:84057/3‑149 (MQ=255)
                                                                                                                                aGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTGGGGAGTGACAGCGGGGGCACTGCAATg              <  1:132753/148‑1 (MQ=255)
                                                                                                                                 ggCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTGGGGAGTGACAGCGGGGGCACTGCAATGa             >  7:37949/1‑148 (MQ=255)
                                                                                                                                  gCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTGGGGAGTGACAGCGGGGGCACTGCAATGACa           >  3:269635/1‑149 (MQ=255)
                                                                                                                                       gTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTGGGGAGTGACAGCGGGGGCACTGCAATGACATgggg      <  2:70586/149‑1 (MQ=255)
                                                                                                                                             cAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTGGGGAGTGACAGCGGGGGCACTGCAATGACATGGGGCGaa  <  4:269635/147‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CCAGGACATGACGGTCCAGCGACTCGCCGAGCTGCGCGCCAATCGCACAACCACCGACGCTTCCGGCCAGGCCGCCGAGAATGGCGCCGGATACCGAGCCGAGGGTGATGCCGACAGGGCCGGCGATTAGGCCGAGTGCTGCAC‑CGGCCTTGCCGCCTGCCAGAGCAGCGCTGACGCCACGTGCGAGGCCTCCGACAGCGCCGATGGCCGCGCCGGCTTTCATGGCGTGATGGAATGAAGCGACTTTTGGGGAGTGACAGCGGGGGCACTGCAATGACATGGGGCGAA  >  NC_002947/2845836‑2846123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑