| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,100,562:1 | +C | 7.0% | coding (1341/1470 nt) | PP_2709 → | long‑chain fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,100,562 | 1 | . | C | 7.0% | 220.2 / 9.8 | 57 | coding (1341/1470 nt) | PP_2709 | long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base . (28/25); new base C (2/2); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
AAGTGGTGTGATCGCCCAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCTT > NC_002947/3100415‑3100707 | aaGTGGTGTGATCGCCCAGGCTTTCGTGCTTGTCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGTCATCAGAGACGAGGCCATCGATCAGGGCGTACAGCAATGCAACGCGCGATTGCGCGATTACGCCAgg‑g < 3:35109/149‑1 (MQ=255) gtgAGCGCCCCTGGTTTCGGTGATGGCGTAGCCTTGCCCCGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGc < 8:52766/149‑1 (MQ=255) aTCGCCCAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGGCGTGCA‑ccctg > 7:322479/1‑145 (MQ=255) cccAGGCTTTCTTGCATGGCGAAGCCTTGCCACGCAACCATGCCTTGATCTGGCCGCTGGATCCCGGCATCAGCGACGAGCCCATCGAACAGGCCGTACAGCAATGCAACGCGCGAATGCCCGATTACGCCAGG‑GTGCA‑CGCCTGgcgc > 1:137775/1‑149 (MQ=255) cccAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGgcgc > 8:86175/1‑149 (MQ=255) cccAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGgcgc > 7:75185/1‑149 (MQ=255) aGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGcgccgc > 8:183880/1‑149 (MQ=255) gCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGGCGTGCA‑C‑CCTGgcgtaatcg < 8:322479/149‑7 (MQ=255) tGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGGCGTGCA‑C‑CCTGgcg < 2:138728/136‑1 (MQ=255) tGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGGCGTGCA‑C‑CCTGgcg > 1:138728/1‑136 (MQ=255) cATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGc < 3:180943/148‑1 (MQ=255) cGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCctct > 1:93996/1‑147 (MQ=255) cGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTa < 3:185745/149‑1 (MQ=255) cTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGc < 8:75185/149‑1 (MQ=255) ttGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCt > 5:243213/1‑149 (MQ=255) gCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGTCAGTGCCTCTTAGCACGCtt < 4:192021/148‑1 (MQ=255) aCGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGc > 6:106835/1‑131 (MQ=255) aCGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGc < 5:106835/131‑1 (MQ=255) aaCCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAAc > 7:217527/1‑149 (MQ=255) ccttgccttgCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAAccc > 3:112138/1‑149 (MQ=255) ttgccttgcTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGaaa < 3:303808/144‑1 (MQ=255) ttgccttgcTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGaaa > 4:303808/1‑144 (MQ=255) ccttgcTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGAc < 4:20909/148‑1 (MQ=255) tgcTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CTCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGc > 3:319683/1‑148 (MQ=255) gcTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGc < 7:183880/147‑1 (MQ=255) ctctGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCaa > 7:136965/1‑149 (MQ=255) gCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGgc < 3:135091/148‑1 (MQ=255) cGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGgccgc > 7:159302/1‑149 (MQ=255) gCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGgccgcc > 8:216451/1‑149 (MQ=255) ccGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGc > 5:265016/1‑130 (MQ=255) ccGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGc < 6:265016/130‑1 (MQ=255) ccGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGt > 2:224949/1‑149 (MQ=255) cGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGt > 7:272504/1‑148 (MQ=255) gCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAg < 7:216451/149‑1 (MQ=255) cAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGa > 5:3738/1‑149 (MQ=255) ggCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGc < 6:243213/149‑1 (MQ=255) gCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CTCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCg < 4:319683/149‑1 (MQ=255) ccATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGt < 1:224949/149‑1 (MQ=255) cATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGt > 8:105133/1‑148 (MQ=255) cATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGt > 7:268519/1‑148 (MQ=255) cATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATAGCCGCCCGCGACGTGAGGCGATCTTGCAGCGt > 2:31291/1‑148 (MQ=255) aTCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTa > 3:140634/1‑149 (MQ=255) aTCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTa > 7:85901/1‑149 (MQ=255) aCAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACcaca < 4:112138/149‑1 (MQ=255) cAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCacac > 6:280606/1‑149 (MQ=255) ccGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCtg < 8:268519/149‑1 (MQ=255) cAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGa < 6:3738/149‑1 (MQ=255) aTGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACa < 4:140634/149‑1 (MQ=255) aTGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACa < 8:217527/149‑1 (MQ=255) gCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCAGCGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGGGGCGATCTTg > 1:232086/1‑120 (MQ=255) gCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGGGGCGATCTTg < 2:232086/119‑1 (MQ=255) gcgcgATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGtt < 5:280606/149‑1 (MQ=255) gcgcgATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTCCCTCTTAGCTCGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGtt < 2:93996/149‑1 (MQ=255) aTTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAg < 7:86175/149‑1 (MQ=255) gCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTcc > 7:34460/1‑149 (MQ=255) ccAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTc < 1:117591/147‑1 (MQ=255) ccAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTc > 2:117591/1‑147 (MQ=255) cAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCtt > 3:179260/1‑149 (MQ=255) | AAGTGGTGTGATCGCCCAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCA‑CGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCTT > NC_002947/3100415‑3100707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |