Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,082,621 G→C 6.2% G189A (GGC→GCC)  PP_3592 → RpiR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,082,6210GC 6.2% 206.9 / 3.9 65G189A (GGC→GCC) PP_3592RpiR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (29/32);  new base C (2/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

ACGTGGCGCAACGCCTGGCGAGCAAGCCGCGGGTGTTCATCGCCGGCTTCCAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGC  >  NC_002947/4082477‑4082768
                                                                                                                                                 |                                                                                                                                                   
aCGTGGCGCAACGCCTGGCGAGCAAGCCGCGGGTGTTCATCGCCGGCTTCCAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGaa                                                                                                                                                 <  7:341244/149‑1 (MQ=255)
    ggCGCAACGCCTGGCGAGCAAGCCGCGGGTGTTCATCGCCGGCTTCCAGACCGAGCGCGGCATCGCCGTAGTGCATGAGCCACCTGCTGCAATAACTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGtg                                                                                                                                              >  1:293600/1‑149 (MQ=255)
       gcAACGCCTGGCGAGCAAGCCGCGGGTGTTCATCGCCGGCTTCCAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGCCGAAGtgcccg                                                                                                                                          <  6:265183/149‑4 (MQ=255)
         aaCGCCTGGCGAGCAAGCCGCGGGTGTTCATCGCCGGCTTCCAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGctgctg                                                                                                                                        <  2:346583/149‑1 (MQ=255)
                 gCGAGCAAGCCGCGGGTGTTCATCGCCGGCTTCCAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGt                                                                                                                                  <  1:203105/147‑1 (MQ=255)
                 gCGAGCAAGCCGCGGGTGTTCATCGCCGGCTTCCAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGc                                                                                                                                >  8:110269/1‑149 (MQ=255)
                 gCGAGCAAGCCGCGGGTGTTCATCGCCGGCTTCCAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGc                                                                                                                                <  3:95257/149‑1 (MQ=255)
                     gCAAGCCGCGGGTGTTCATCGCCGGCTTCCAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGaa                                                                                                                            <  4:251706/149‑1 (MQ=255)
                         gCCGCGGGTGTTCATCGCCGGCTTCCAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACa                                                                                                                        <  8:263557/149‑1 (MQ=255)
                                     cATCGCCGGCTTCCACACCGAGCGCGGCTTCCCCAT‑GTGCATGAGCCACCTGCTGCAATACCTCCCCGATGGCGTGCAACTCCTCGACATCACCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCTAAGACAGCGCCCtggtgg                                                                                                            <  2:293600/149‑1 (MQ=255)
                                      aTCGCCGGCTTCCAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCTGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTggtggt                                                                                                           >  5:125236/1‑149 (MQ=255)
                                      aTCGCCGGCTTCCAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTggtggt                                                                                                           <  3:124990/149‑1 (MQ=255)
                                         gCCGGCTTCCAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGgtgt                                                                                                         <  3:121864/148‑1 (MQ=255)
                                          ccGGCTTCCAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTc                                                                                                       >  1:275964/1‑149 (MQ=255)
                                               ttCCAGCCCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATCGCGTGCAACTGGACGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTCACGAAGACAGCGccc                                                                                                                  >  8:133418/1‑133 (MQ=255)
                                               ttCCAGACCGAGCGCGGCATCGCGAT‑GTGCATGAGCCACCTGCTGCAATAGCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCAGTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGccc                                                                                                                  <  7:133418/133‑1 (MQ=255)
                                                 ccAGACGGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGa                                                                                                     <  7:324748/144‑1 (MQ=255)
                                                 ccAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGa                                                                                                     >  8:324748/1‑144 (MQ=255)
                                                 ccAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGgcgc                                                                                                >  8:71991/1‑149 (MQ=255)
                                                   aGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGgcgcgc                                                                                              <  4:25230/149‑1 (MQ=255)
                                                   aGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGgcgcgc                                                                                              >  6:282303/1‑149 (MQ=255)
                                                   aGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGgcgcgc                                                                                              >  7:327412/1‑149 (MQ=255)
                                                     aCCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGtt                                                                                                        >  6:84304/1‑137 (MQ=255)
                                                     aCCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGtt                                                                                                        <  5:84304/137‑1 (MQ=255)
                                                      ccGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGt                                                                                           <  7:110269/149‑1 (MQ=255)
                                                         aGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTAt                                                                                        >  8:10509/1‑149 (MQ=255)
                                                                  tCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCTGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCAt                                                                               <  6:125236/149‑1 (MQ=255)
                                                                  tCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCAt                                                                               <  5:282303/149‑1 (MQ=255)
                                                                  tCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCAt                                                                               >  7:181428/1‑149 (MQ=255)
                                                                  tCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCAt                                                                               >  3:13331/1‑149 (MQ=255)
                                                                     ccAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGcc                                                                            >  7:77609/1‑149 (MQ=255)
                                                                                  gCCACCTGCTGCAATGCCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTg                                                                                                                                 >  8:172244/1‑84 (MQ=255)
                                                                                  gCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGc                                                                <  2:275964/149‑1 (MQ=255)
                                                                                   ccACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGcc                                                               <  2:337345/149‑1 (MQ=255)
                                                                                   ccACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGc                                                                >  6:169829/1‑148 (MQ=255)
                                                                                      ccTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGa                                                            <  5:169829/149‑1 (MQ=255)
                                                                                          ctgcAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAg                                                          <  8:77609/147‑1 (MQ=255)
                                                                                             cAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGgcgc                                                      <  4:13331/148‑1 (MQ=255)
                                                                                                 aCCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAg                                                 >  6:24464/1‑149 (MQ=255)
                                                                                                   cTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGa                                                                        <  3:277676/124‑1 (MQ=255)
                                                                                                   cTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGa                                                                        >  4:277676/1‑124 (MQ=255)
                                                                                                   cTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGa                                                  >  6:266452/1‑146 (MQ=255)
                                                                                                   cTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGa                                                  <  5:266452/146‑1 (MQ=255)
                                                                                                   cTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGc                                               >  3:359736/1‑149 (MQ=255)
                                                                                                         gATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGc                                           <  8:189858/147‑1 (MQ=255)
                                                                                                         gATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGc                                           >  7:189858/1‑147 (MQ=255)
                                                                                                               gTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTg                                             >  3:215957/1‑139 (MQ=255)
                                                                                                               gTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTg                                             <  4:215957/139‑1 (MQ=255)
                                                                                                               gTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGATGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGt                                   <  8:327412/149‑1 (MQ=255)
                                                                                                                           gACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCAt                                         <  6:120000/131‑1 (MQ=255)
                                                                                                                           gACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCAt                                         >  5:120000/1‑131 (MQ=255)
                                                                                                                           gACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTGCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGa                        <  7:234552/148‑1 (MQ=255)
                                                                                                                            aCATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCAGGGTGAcc                      >  8:336801/1‑149 (MQ=255)
                                                                                                                            aCATCAGCGCCGGGCAATTCGGAGAATTGCTGCTGGGACTTGACGAAGAAAGAGACCTGTTGGTGTTAGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGTCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGAcc                      >  4:117330/1‑149 (MQ=255)
                                                                                                                              aTCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCAGGGTGACCgc                    <  7:336801/149‑2 (MQ=255)
                                                                                                                               tCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCAGGGTGACCgc                    <  6:16692/148‑2 (MQ=255)
                                                                                                                               tCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCAGGGTGACCgc                    >  5:16692/1‑147 (MQ=255)
                                                                                                                                 aGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGcc                                                                                 <  6:266978/85‑1 (MQ=255)
                                                                                                                                 aGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGcc                                                                                 >  5:266978/1‑85 (MQ=255)
                                                                                                                                    cagcagCACTTCGCCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCtt              >  2:12510/6‑149 (MQ=255)
                                                                                                                                      gcagCACTTCGCCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTCCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGAcc                      <  7:16056/136‑1 (MQ=255)
                                                                                                                                      gcagCACTTCGCCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTACCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGAcc                      >  8:16056/4‑139 (MQ=255)
                                                                                                                                        ggCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCtgt          <  8:181428/149‑1 (MQ=255)
                                                                                                                                             ttCGGCGAAGTGCTGCTGGGCCGTGCCGTAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTACCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTg     >  1:205150/1‑149 (MQ=255)
                                                                                                                                             ttCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTg     >  7:327646/1‑149 (MQ=255)
                                                                                                                                                ggCTAAGTGCTGCTTGGCCGTGCCTAATACAGCGCCCTGGTTGTTTTCGAGGCGCTCCGTTATTACCGCCATGCCCTGATGCTGTGCCATAAGGCGAGCGATGCTGGCATTGCGGTCACACTGTTGACCGACACCTTCTGTGACTgttc  <  3:117330/149‑4 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ACGTGGCGCAACGCCTGGCGAGCAAGCCGCGGGTGTTCATCGCCGGCTTCCAGACCGAGCGCGGCATCGCCAT‑GTGCATGAGCCACCTGCTGCAATACCTGCGCGATGGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGC  >  NC_002947/4082477‑4082768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑