Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,125,301 A→T 8.9% intergenic (+30/+51) PP_3628 → / ← PP_3629 choline/carnitine/betaine transporter family protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,125,3010AT 8.9% 161.4 / 5.9 56intergenic (+30/+51)PP_3628/PP_3629choline/carnitine/betaine transporter family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/25);  new base T (3/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-01

AGAAGGCCGTACTGTATGGCGCGGTGAAGCATGGGGAGGAGTTCTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGAACGCACGCTTGGCCGCCGCACGGTCGGGG  >  NC_002947/4125159‑4125445
                                                                                                                                              |                                                                                                                                                
agaAGGCCGTACTGTATGGCGCGGTGAAGCATGGGGAGGAGTTCTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAagcagc                                                                                                                                            >  3:136420/1‑149 (MQ=255)
   aGGCCGTACTGTATAGCGCGGTGAAGCATGGGGAGGAGTTCTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTTAGCAGCCCg                                                                                                                                         <  5:225259/149‑1 (MQ=255)
    ggCCGTACTGTATGGCGCGGTGAAGCATGGGGAGGAGTTCTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTTAGCAGCCCGt                                                                                                                                        <  2:298603/149‑1 (MQ=255)
      ccGTACTGTATGGCGCGGTGAAGCATGGGGAGGAGTTCTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTc                                                                                                                                      <  2:157417/149‑1 (MQ=255)
           cTGTATGGCGCGGTGAAGCATGGGGAGGAGTTCTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGa                                                                                                                                  <  7:92085/148‑1 (MQ=255)
              tATGGCGCGGTGAAGCATGGGGAGGAGTTCTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGc                                                                                                                               <  7:46209/148‑1 (MQ=255)
               aTGGCGCGGTGAAGCATGGGGAGGAGTTCTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGc                                                                                                                               >  8:294336/1‑147 (MQ=255)
                  gcgcGGTGAAGCATGGGGAGGAGTTCTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCgaga                                                                                                                           <  7:162365/148‑1 (MQ=255)
                                   gaggagTTCTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACAt                                                                                                         >  4:175228/1‑149 (MQ=255)
                                   gaggagTTCTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACAt                                                                                                         >  2:60070/1‑149 (MQ=255)
                                       agTTCTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGAt                                                                                                     <  1:60070/149‑1 (MQ=255)
                                       agTTCTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGAt                                                                                                     <  2:93798/149‑1 (MQ=255)
                                           cTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGc                                                                                                 >  8:7218/1‑149 (MQ=255)
                                                     gtgCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCtt                                                                                        <  7:7218/148‑1 (MQ=255)
                                                      tgCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTcc                                                                                      <  1:360539/149‑1 (MQ=255)
                                                           gagaAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAAcgc                                                                                 >  2:132039/1‑149 (MQ=255)
                                                            agaagaAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTACCCCGCAACTGACGGCGTCAACCCGAAACCCACGGGCTGCTAAGCAGCCCGTGCGCGATGCGAGACCGCGCCTGCGGAAACATGGCTCTGCGGCAACCGTCCACcgcc                                                                                >  7:323857/1‑149 (MQ=255)
                                                             gaagaaGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAAcgccg                                                                               >  2:318419/1‑149 (MQ=255)
                                                                gaagGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGa                                                                                                      >  3:119621/1‑123 (MQ=255)
                                                                gaagGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGa                                                                                                      <  4:119621/123‑1 (MQ=255)
                                                                gaagGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAAcgccgccg                                                                            >  6:264364/1‑149 (MQ=255)
                                                                gaagGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAAcgccgccg                                                                            >  1:135423/1‑149 (MQ=255)
                                                                      aTCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCt                                                                      >  1:181255/1‑149 (MQ=255)
                                                                         ggCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTg                                                                    <  7:294336/148‑1 (MQ=255)
                                                                             aaGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCca                                                                >  6:285628/1‑148 (MQ=255)
                                                                                aCAGCGGCAGAGGCCGAAGTAACCCTCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATc                                                            <  4:234709/149‑1 (MQ=255)
                                                                                  aGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGGTGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGa                                                          >  4:269382/1‑149 (MQ=255)
                                                                                   gCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCTACATCGAt                                                         <  2:181255/149‑1 (MQ=255)
                                                                                       cAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTg                                                     >  7:67134/1‑149 (MQ=255)
                                                                                         gagGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCt                                                                          <  6:122735/126‑1 (MQ=255)
                                                                                         gagGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCt                                                                          >  5:122735/1‑126 (MQ=255)
                                                                                            gCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCTCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACAt                                                             <  8:270297/136‑1 (MQ=255)
                                                                                            gCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACAt                                                             >  7:270297/1‑136 (MQ=255)
                                                                                             ccGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCt                                                                                                <  1:204047/100‑1 (MQ=255)
                                                                                             ccGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCt                                                                                                >  2:204047/1‑100 (MQ=255)
                                                                                              cGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCt                                              <  4:136420/149‑1 (MQ=255)
                                                                                                   tAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGcatc                                         <  1:132039/149‑1 (MQ=255)
                                                                                                     aCCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGcatcat                                       <  8:67134/149‑1 (MQ=255)
                                                                                                       ccGCAACTGACGGCGTCAACACGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGCTCTGCTTCAACCTTCCAACGCCGCAGCTATCATGGCCCCAACGATCTTGCCCAAATGCAGCATgg                                     >  3:7893/1‑149 (MQ=255)
                                                                                                        cGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAAcgccgccgc                                                                           <  4:222469/110‑1 (MQ=255)
                                                                                                        cGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAAcgccgccgc                                                                           >  3:222469/1‑110 (MQ=255)
                                                                                                        cGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGc                                    <  2:135423/149‑1 (MQ=255)
                                                                                                        cGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGc                                    <  3:175228/149‑1 (MQ=255)
                                                                                                              tGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGaa                              >  4:176005/1‑149 (MQ=255)
                                                                                                                  ggCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGAacgca                          >  4:191694/1‑149 (MQ=255)
                                                                                                                     gTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGAacgcacgc                       <  1:318419/149‑1 (MQ=255)
                                                                                                                         aCCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGAACGCACGCTTgg                   >  1:208800/1‑149 (MQ=255)
                                                                                                                         aCCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGAACGCACGCTTgg                   <  3:191694/149‑1 (MQ=255)
                                                                                                                          cccGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGACCGCACGCTTGgc                  >  2:168787/1‑149 (MQ=255)
                                                                                                                             gAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGAACGCACGCTTGgccgc               >  3:341092/1‑149 (MQ=255)
                                                                                                                                  gaaCGGGCTGCTTAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGAACGCACGCTTGGCCGCCGCAc          >  7:302811/3‑149 (MQ=255)
                                                                                                                                  gaaCGGGCTGCTTAGCAGCCCGTTCGCGATGCGAGAACGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGAACGCACGCTTGGCCGCCGCAc          >  3:295852/3‑149 (MQ=255)
                                                                                                                                  ccACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGAACGCACGCTTGGCCGCCGCAc          >  4:288632/1‑149 (MQ=255)
                                                                                                                                    aCGGGCTGCTTAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGAACGCACGCTTGGCCGCCGCACgg        >  5:82939/1‑149 (MQ=255)
                                                                                                                                         ctgctAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGAACGCACGCTTGGCCGCCGCACGGTCggg   <  4:341092/149‑1 (MQ=255)
                                                                                                                                            ctAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGAACGCACGCTTGGCCGCCGCACGGTCgggg  <  5:264364/147‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
AGAAGGCCGTACTGTATGGCGCGGTGAAGCATGGGGAGGAGTTCTACATTGTGGTGCAGGAGAAGAAGGCATCGGCCAAGACAGCGGCAGAGGCCGAAGTAACCCGCAACTGACGGCGTCAACCCGAAAACCACGGGCTGCTAAGCAGCCCGTTCGCGATGCGAGACCGCGCCTGCTGAAACATGGATCTGCTTCAACCTTCCAACGCCGCCGCTATCTTGGCCACATCGATCTTGCCCATCTGCATCATGGCCTCGAACGCACGCTTGGCCGCCGCACGGTCGGGG  >  NC_002947/4125159‑4125445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑