| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 326,065 | C→A | 13.1% | *556Y (TAG→TAT) | PP_0269 ← | glutamate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 326,065 | 0 | C | A | 13.1% | 204.5 / 18.8 | 76 | *556Y (TAG→TAT) | PP_0269 | glutamate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base C (32/34); new base A (5/5); total (37/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
TCGATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTC > NC_002947/325918‑326208 | tCGATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGcc > 7:239252/1‑148 (MQ=255) gATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTAt < 8:239252/149‑1 (MQ=255) aGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCAAACAGCCCCGGCATAGGCCgg > 6:246181/1‑149 (MQ=255) cTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCgggg > 3:48649/1‑149 (MQ=255) cTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCAAACAGCCCCGGCATAGGCCgggg < 2:90176/149‑1 (MQ=255) tGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGc > 3:33829/1‑149 (MQ=255) gCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCATAGGCCGGGGCt < 7:118182/149‑1 (MQ=255) aGCTCGTTCTAGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCAAACAGCCCCGGCATAGGCCGGGGCTGtt > 2:67789/1‑148 (MQ=255) cTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCAAACAGCCCCGGCATAGGCCGGGGCTGttcga < 1:67789/149‑4 (MQ=255) tCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCtt > 8:26262/1‑149 (MQ=255) gTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCtttt < 4:33829/149‑1 (MQ=255) ttCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTc < 2:31921/149‑1 (MQ=255) cTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCg < 4:211093/148‑1 (MQ=255) gCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCgg < 5:203652/146‑1 (MQ=255) aCCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTAATCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACgg > 1:65874/1‑149 (MQ=255) ccAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGt < 7:26262/149‑1 (MQ=255) caacaaCGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTGCATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGt < 1:276451/149‑1 (MQ=255) caacaaCGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGt > 6:153409/1‑148 (MQ=255) aacaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGtt > 5:83804/1‑148 (MQ=255) aacaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTa < 7:206613/149‑1 (MQ=255) acaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTAc < 7:114974/149‑1 (MQ=255) acaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTAc > 2:230406/1‑149 (MQ=255) acaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCAGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTAAGCCGGGGCTGTTTCTTTTCGGCGAACGGTTAc > 3:80848/1‑149 (MQ=255) acGCCGTTCATCATTACGGTTACAAAGAAGTCGGCAAAGAATTCCGCGTATTCGTCGATTAACCGATCATCATCTTCTTTTTTCCTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACt < 1:289729/149‑1 (MQ=255) acGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGAGTATTCGTCGAGTACCAGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCCGCCTATGCAGGGGCTGTTTCTTTTCGGAGAACGCTTACTGt > 4:231192/1‑149 (MQ=255) gCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGttt < 4:48649/149‑1 (MQ=255) ccGTGCAGCATATCGGTTACAACGACGACGGCAACGAAGTCCGAGTATTCGTCGAGTAGCCTATCAGCATCTTGTGATT‑‑CTGATCTG‑ATGTATCTAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTg < 4:80848/149‑1 (MQ=255) cGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGttt < 6:83804/147‑1 (MQ=255) cGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGa < 3:351042/149‑1 (MQ=255) cGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGa < 5:153409/149‑1 (MQ=255) agAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGgcgc > 2:59991/1‑149 (MQ=255) gAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCt > 1:155827/1‑149 (MQ=255) aCGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTgg > 7:208430/1‑149 (MQ=255) aCGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTGCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTgg < 8:307206/149‑1 (MQ=255) cGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCTAGTACCCGATGAGCAGCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTggg < 3:231192/149‑1 (MQ=255) cAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑Ac < 1:230406/148‑1 (MQ=255) aaCGACGACGGCAAAGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCGTCTGATT‑‑ATGATCTG‑ATGTATCGAACAGCACCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACCGAGACTGTTTGAGCCTGCGCTGGGCAT‑ACgg > 8:268042/1‑149 (MQ=255) acgacgGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGtctc < 1:59991/149‑1 (MQ=255) gacgGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCg < 8:208430/149‑1 (MQ=255) acgGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCgg < 7:3692/149‑1 (MQ=255) cgGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGt > 2:69867/1‑149 (MQ=255) aaCGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCa > 3:196057/1‑148 (MQ=255) aCGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCaaa < 2:65874/149‑1 (MQ=255) gcttATTCGGCGAGTACCCGATCTGCTTCTTCTGATT‑‑CTGATCTG‑ATGTTTCGACCAGCCCCGGCAATTGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCt < 2:155827/146‑1 (MQ=255) cgTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTa > 5:100251/1‑149 (MQ=255) aTTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTAcgc > 5:112625/1‑149 (MQ=255) tcgtcgAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGTTCTCAGGTGCAAAGATATCGATAcgccc > 6:30176/1‑147 (MQ=255) gtcgAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCa > 5:230181/1‑149 (MQ=255) gAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATcc > 4:224805/1‑149 (MQ=255) gATCACCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTAACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACAt < 1:69867/149‑1 (MQ=255) aTCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCa > 8:146050/1‑148 (MQ=255) ttctgatt‑‑ctgaTCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATaa > 6:170615/1‑148 (MQ=255) ctgatt‑‑ctgatCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATaaaa < 5:170615/148‑1 (MQ=255) gatg‑‑ctgatCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTc < 7:171558/149‑1 (MQ=255) tt‑‑ctgatCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGCCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCg > 1:47701/1‑148 (MQ=255) tt‑‑ctgatCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGCCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCg < 2:47701/148‑1 (MQ=255) t‑‑ctgatctg‑aTGTATCGAACAGCCCCGGCCTATGACGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCt > 1:316547/1‑149 (MQ=255) atctg‑ttGTATCGAACAGCCCCGGGCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtc < 7:268042/148‑1 (MQ=255) tctg‑atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAAc < 5:114031/54‑1 (MQ=255) tctg‑atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAAc > 6:114031/1‑54 (MQ=255) atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgat < 6:230181/146‑1 (MQ=255) gTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCg < 5:353611/136‑1 (MQ=255) gTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCg > 6:353611/1‑136 (MQ=255) tATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCGT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCCCATCCGCGCATAAAACTCGCTTTCGATCGAGcc > 8:185790/1‑149 (MQ=255) aTCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCa > 5:22608/1‑149 (MQ=255) tCGAACAGCCCCGGCCTATGGCGGGGCTTTTTCTTTTGGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCAc < 5:30176/149‑1 (MQ=255) tCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACTGTTACTGTTTGAGCCGGCGCTTGGCTT‑AAGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCAc < 3:248036/149‑1 (MQ=255) aaCAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCgcgc < 5:161599/120‑1 (MQ=255) aaCAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCgcgc > 6:161599/1‑120 (MQ=255) aCAGCCCCGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAg > 2:260421/1‑149 (MQ=255) aGCCCCGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGt > 2:103355/1‑148 (MQ=255) ccccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAAc > 4:71475/1‑149 (MQ=255) ccccGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACt > 4:124349/1‑142 (MQ=255) ccccGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACt < 3:124349/142‑1 (MQ=255) cccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACt > 6:350817/1‑149 (MQ=255) cccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCAACTGAGTAACt > 5:276098/1‑149 (MQ=255) ccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTc < 7:146050/149‑1 (MQ=255) | TCGATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATT‑‑CTGATCTG‑ATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTT‑ACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTC > NC_002947/325918‑326208 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |